Overview
- External ontology
- TOGO:M865 — Halarchaeum Neutrophilum Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M865 Original source: JCM - JCM_M829-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=829
Composition
flowchart LR
CultureMech_010282["halarchaeum_neutrophilum_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010282 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_010282 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010282 --> ing2_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_6H2O -.-> CHEBI_86345
ing3_KCl["KCl"]
CultureMech_010282 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_Glucose["Glucose"]
CultureMech_010282 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_agar["agar"]
CultureMech_010282 --> ing5_agar
CHEBI_2509["CHEBI:2509"]
ing5_agar -.-> CHEBI_2509
ing6_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010282 --> ing6_Yeast_extract__BD_Difco_
ing7_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_010282 --> ing7_Tryptone__BD_Difco_
ing8_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
CultureMech_010282 --> ing8_Casamino_acids__BD_Difco_
ing9_KOH["KOH"]
CultureMech_010282 --> ing9_KOH
CHEBI_32035["CHEBI:32035"]
ing9_KOH -.-> CHEBI_32035
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
180 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
20 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
KCl
Cl.K 74.551 g/mol
|
5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 6 | agar | 20 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 7 | Yeast extract (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 8 | Tryptone (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 9 | Casamino acids (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 10 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.861379Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M865 |
| 2026-01-28T19:48:35.950837Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH |
| 2026-03-13T06:20:12.674096Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010282 |
| 2026-03-17T06:30:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.620930+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Halarchaeum Neutrophilum Medium" to "halarchaeum_neutrophilum_medium" for consistent matching |
| 2026-05-16T09:03:21.539700+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:45.174737+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |