desulfovibrio_b0109p2_medium

CultureMech:010283 bacterial COMPLEX LIQUID
Original name: Desulfovibrio B0109P2 Medium

Overview

External ontology
TOGO:M866 — Desulfovibrio B0109P2 Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M866 Original source: JCM - JCM_M830 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=830

Composition

flowchart LR
    CultureMech_010283["desulfovibrio_b0109p2_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010283 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_010283 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_010283 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_010283 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_KH2PO4["KH2PO4"]
    CultureMech_010283 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_NH4Cl["NH4Cl"]
    CultureMech_010283 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_K2HPO4["K2HPO4"]
    CultureMech_010283 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_Resazurin["Resazurin"]
    CultureMech_010283 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_KCl["KCl"]
    CultureMech_010283 --> ing8_KCl
    CHEBI_32588["CHEBI:32588"]
    ing8_KCl -.-> CHEBI_32588
    ing9_Na2SO4["Na2SO4"]
    CultureMech_010283 --> ing9_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing9_Na2SO4 -.-> CHEBI_32149
    ing10_Na2S2O3_5H2O["Na2S2O3・5H2O"]
    CultureMech_010283 --> ing10_Na2S2O3_5H2O
    CHEBI_32150["CHEBI:32150"]
    ing10_Na2S2O3_5H2O -.-> CHEBI_32150
    ing11_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
    CultureMech_010283 --> ing11_L__Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing11_L__Cysteine_HCl_H2O -.-> CHEBI_91248
    ing12_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_010283 --> ing12_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing12_Carbon_dioxide_gas -.-> CHEBI_16526
    ing13_Nitrogen_gas["Nitrogen gas"]
    CultureMech_010283 --> ing13_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing13_Nitrogen_gas -.-> CHEBI_17997
    ing14_1_0_M_Sodium_lactate["1.0 M Sodium lactate"]
    CultureMech_010283 --> ing14_1_0_M_Sodium_lactate

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
900 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 Yeast extract 0.1 G_PER_L Role: Nutrient source; Properties: Undefined component
3 NaCl
Cl.Na 58.443 g/mol
20 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
4 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
5 KH2PO4
H2O4P.K 136.084 g/mol
0.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
6 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
7 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
8 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Defined component, Simple component, Organic compound
9 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
10 Na2SO4
2Na.O4S 142.043 g/mol
2.8 G_PER_L CHEBI:32149
sodium sulfate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
11 Na2S2O3・5H2O
5H2O.2Na.O3S2 248.186 g/mol
3.16 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
 Properties: Defined component, Inorganic compound, Simple component
12 L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Defined component, Simple component, Organic compound
13 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
14 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
15 1.0 M Sodium lactate 20 G_PER_L Role: Carbon source; Properties: Organic compound, Solution, Defined component

Solutions

Trace element solution (see Medium [M439])

Dose: 1 G_PER_L

Cross-reference to Medium M439

5% NaHCO3 solution

Dose: 40 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

15% MgCl2・6H2O solution

Dose: 20 G_PER_L

Role: Mineral source; Properties: Inorganic compound, Solution, Defined component

2% Na2S・9H2O solution

Dose: 20 G_PER_L

Role: Reducing agent; Properties: Solution, Inorganic compound, Defined component

NaCO3 solution

Dose: variable VARIABLE

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.862813Z togo-import Imported from TOGO Medium Source: JCM, ID: M866
2026-01-28T19:48:16.238611Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: NaCO3 solution; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:26.808154+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 5 solution(s) from ingredients
2026-03-13T03:08:55.473177Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:20:12.683506Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010283
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:59.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:31.629895+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Desulfovibrio B0109P2 Medium" to "desulfovibrio_b0109p2_medium" for consistent matching
2026-05-16T09:03:21.550227+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:45.185300+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:51.875547+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.