Overview
- External ontology
- TOGO:M868 — R2A Agar With Artificial Seawater
- Applications
-
- Microbial cultivation
- Notes
- TOGO M868 mirrors JCM Medium 832: 18.2 g R2A agar (BD-Difco) with 1.0 L artificial seawater from JCM Medium 736.
Composition
flowchart LR
CultureMech_010285["r2a_agar_with_artificial_seawater"]
ing0_R2A_agar__BD_Difco_["R2A agar (BD-Difco)"]
CultureMech_010285 --> ing0_R2A_agar__BD_Difco_
Ingredients (1)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | R2A agar (BD-Difco) | 18.2 G_PER_L | JCM Medium 832 lists 18.2 g R2A agar (BD-Difco) per liter of artificial seawater. |
Preparation
- Prepare artificial seawater from the JCM Medium 736 salts.
- Dissolve 18.2 g R2A agar (BD-Difco) in 1.0 L artificial seawater.
- Autoclave at 121 C for 15 min.
Solutions
Artificial seawater
Dose: 1000 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaCl | 23.5 G_PER_L | JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 832. |
| MgCl2 x 6 H2O | 4.9 G_PER_L | JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 832. |
| Na2SO4 | 3.9 G_PER_L | JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 832. |
| CaCl2 x 2 H2O | 1.1 G_PER_L | JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 832. |
| KCl | 0.66 G_PER_L | JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 832. |
| NaHCO3 | 0.19 G_PER_L | JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 832. |
| KBr | 0.096 G_PER_L | JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 832. |
| H3BO3 | 0.026 G_PER_L | JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 832. |
| SrCl2 x 6 H2O | 0.024 G_PER_L | JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 832. |
| NaF | 0.003 G_PER_L | JCM Medium 736 prints this component in the Artificial seawater subrecipe used by JCM Medium 832. |
| Distilled water | 1000 ML_PER_L | JCM Medium 736 lists 1.0 L distilled water for the Artificial seawater subrecipe. |
JCM Medium 832 adds 1.0 L artificial seawater per liter of R2A Agar With Artificial Seawater; the salt composition is printed in JCM Medium 736.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.867440Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M868 |
| 2026-03-09T08:04:18.455443+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:12.699995Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010285 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T19:46:31.646236+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "R2A Agar With Artificial Seawater" to "r2a_agar_with_artificial_seawater" for consistent matching |
| 2026-05-16T09:03:21.569648+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-09-06T00:00:00-07:00 | repair_r2a_score40.py | RESOLVED_R2A_SCORE40_GRAPH | TOGO M868 mirrors JCM Medium 832: 18.2 g R2A agar (BD-Difco) with 1.0 L artificial seawater from JCM Medium 736. |