modified_desulfovibrio_medium

CultureMech:010288 bacterial COMPLEX LIQUID
Original name: Modified Desulfovibrio Medium

Overview

External ontology
TOGO:M870 — Modified Desulfovibrio Medium
Applications
  • Microbial cultivation
pH range
{'min': 6.8, 'max': 7.0, 'notes': 'JCM Medium 389 says adjust pH to 6.8-7.0, if necessary.'}
Notes
TOGO M870 mirrors JCM Medium 834: Modified Desulfovibrio Medium uses JCM Medium 389 supplemented with 3.0 g/L Beef extract (BD-Difco).

Composition

flowchart LR
    CultureMech_010288["modified_desulfovibrio_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_010288 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_NH4Cl["NH4Cl"]
    CultureMech_010288 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_Na2SO4["Na2SO4"]
    CultureMech_010288 --> ing2_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing2_Na2SO4 -.-> CHEBI_32149
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_010288 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_010288 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Sodium_lactate["Sodium lactate"]
    CultureMech_010288 --> ing5_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing5_Sodium_lactate -.-> CHEBI_75228
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_010288 --> ing6_Yeast_extract
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_010288 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_Sodium_thioglycolate["Sodium thioglycolate"]
    CultureMech_010288 --> ing8_Sodium_thioglycolate
    CHEBI_86481["CHEBI:86481"]
    ing8_Sodium_thioglycolate -.-> CHEBI_86481
    ing9_Ascorbic_acid["Ascorbic acid"]
    CultureMech_010288 --> ing9_Ascorbic_acid
    CHEBI_22652["CHEBI:22652"]
    ing9_Ascorbic_acid -.-> CHEBI_22652
    ing10_Resazurin["Resazurin"]
    CultureMech_010288 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806
    ing11_Distilled_water["Distilled water"]
    CultureMech_010288 --> ing11_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing11_Distilled_water -.-> CHEBI_15377
    ing12_Beef_extract__BD_Difco_["Beef extract (BD-Difco)"]
    CultureMech_010288 --> ing12_Beef_extract__BD_Difco_

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
0.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 JCM Medium 389 prints this component.
2 NH4Cl
Cl.H4N 53.492 g/mol
1.0 G_PER_L CHEBI:31206
ammonium chloride
 JCM Medium 389 prints this component.
3 Na2SO4
2Na.O4S 142.043 g/mol
1.0 G_PER_L CHEBI:32149
sodium sulfate
 JCM Medium 389 prints this component.
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
2.0 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 JCM Medium 389 prints this component.
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
 JCM Medium 389 prints this component.
6 Sodium lactate
C3H5O3.Na 112.06 g/mol
2.0 G_PER_L CHEBI:75228
sodium lactate
 JCM Medium 389 prints this component.
7 Yeast extract 1.0 G_PER_L FOODON:03315426
yeast extract
 JCM Medium 389 prints this component.
8 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.5 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 JCM Medium 389 prints this component.
9 Sodium thioglycolate
C2H3O2S.Na 114.101 g/mol
0.1 G_PER_L CHEBI:86481
sodium thioglycolate
 JCM Medium 389 prints this component.
10 Ascorbic acid 0.1 G_PER_L CHEBI:22652
ascorbic acid
 JCM Medium 389 prints this component.
11 Resazurin
C12H7NO4 229.191 g/mol
0.5 MG_PER_L CHEBI:8806
Resazurin
 JCM Medium 389 prints this component.
12 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 JCM Medium 389 lists 1.0 l distilled water.
13 Beef extract (BD-Difco) 3.0 G_PER_L FOODON:03302088
beef extract
 JCM Medium 834 supplements JCM Medium 389 with 3.0 g/L Beef extract (BD-Difco).

Preparation

  1. Mix ingredients except FeSO4 x 7 H2O, sodium thioglycolate, and ascorbic acid.
  2. Bring the medium to a boil and cool to room temperature under an N2 gas stream.
  3. Add FeSO4 x 7 H2O in 10 mL distilled water plus sodium thioglycolate and ascorbic acid in 10 mL distilled water.
  4. Adjust pH to 7.8 with NaOH; adjust to pH 6.8-7.0 if necessary.
  5. While swirling under an N2 gas stream, dispense into culture vessels and seal with butyl rubber stoppers.
  6. Autoclave the sealed medium.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.872672Z togo-import Imported from TOGO Medium Source: JCM, ID: M870
2026-03-09T08:04:23.740680+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:12.720514Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010288
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T19:46:31.666209+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Desulfovibrio Medium" to "modified_desulfovibrio_medium" for consistent matching
2026-05-16T09:03:21.597701+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-09-06T00:00:00-07:00 repair_jcm_more_wrappers_score40.py RESOLVED_JCM_MORE_SCORE40_GRAPH TOGO M870 mirrors JCM Medium 834: Modified Desulfovibrio Medium uses JCM Medium 389 supplemented with 3.0 g/L Beef extract (BD-Difco).