1_3_lb_agar

CultureMech:010296 bacterial COMPLEX SOLID_AGAR
Original name: 1/3 LB Agar

Overview

External ontology
TOGO:M878 — 1/3 LB Agar
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M878 Original source: JCM - JCM_M842 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=842

Composition

flowchart LR
    CultureMech_010296["1_3_lb_agar"]
    ing0_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
    CultureMech_010296 --> ing0_Tryptone__BD_Difco_
    ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_010296 --> ing1_Yeast_extract__BD_Difco_
    ing2_NaCl["NaCl"]
    CultureMech_010296 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_Agar["Agar"]
    CultureMech_010296 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_Distilled_water["Distilled water"]
    CultureMech_010296 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Tryptone (BD-Difco) 3.3 G_PER_L JCM Medium 842 lists this as the BD-Difco tryptone product; the product is source-disclosed but not reducible to one ChEBI molecule.
2 Yeast extract (BD-Difco) 1.7 G_PER_L JCM Medium 842 lists this as the BD-Difco yeast extract product; the product is source-disclosed but not reducible to one ChEBI molecule.
3 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
 JCM Medium 842 lists 5 g sodium chloride.
4 Agar 15 G_PER_L CHEBI:2509
agar
 JCM Medium 842 lists 15 g agar as the solidifying component.
5 Distilled water
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 JCM Medium 842 lists 1.0 L distilled water.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.884506Z togo-import Imported from TOGO Medium Source: JCM, ID: M878
2026-01-28T19:48:41.618642Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:12.774458Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010296
2026-03-17T06:30:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:31.720821+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "1/3 LB Agar" to "1_3_lb_agar" for consistent matching
2026-06-05T07:40:45.285345+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_lb_family_score15.py RESOLVED_LB_FAMILY_SCORE15 TOGO M878 mirrors JCM Medium 842: 3.3 g Tryptone (BD-Difco), 1.7 g Yeast extract (BD-Difco), 5 g NaCl, 15 g agar, and 1 L distilled water.