Overview
- External ontology
- TOGO:M895 — Pyrolobus Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M895 Original source: JCM - JCM_M859 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=859
Composition
flowchart LR
CultureMech_010312["pyrolobus_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010312 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Resazurin["Resazurin"]
CultureMech_010312 --> ing1_Resazurin
CHEBI_8806["CHEBI:8806"]
ing1_Resazurin -.-> CHEBI_8806
ing2_MES["MES"]
CultureMech_010312 --> ing2_MES
CHEBI_39010["CHEBI:39010"]
ing2_MES -.-> CHEBI_39010
ing3_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_010312 --> ing3_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing3_Carbon_dioxide_gas -.-> CHEBI_16526
ing4_Hydrogen_gas["Hydrogen gas"]
CultureMech_010312 --> ing4_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing4_Hydrogen_gas -.-> CHEBI_18276
ing5_KH2PO4["KH2PO4"]
CultureMech_010312 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_KNO3["KNO3"]
CultureMech_010312 --> ing6_KNO3
CHEBI_63043["CHEBI:63043"]
ing6_KNO3 -.-> CHEBI_63043
ing7_N2["N2"]
CultureMech_010312 --> ing7_N2
CHEBI_17997["CHEBI:17997"]
ing7_N2 -.-> CHEBI_17997
ing8_Na2S_9H2O["Na2S・9H2O"]
CultureMech_010312 --> ing8_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing8_Na2S_9H2O -.-> CHEBI_76209
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
770.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component [Merged 3 duplicates: 750.0, 10.0, 10.0] |
| 2 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component |
| 3 | MES | 1.95 G_PER_L |
CHEBI:39010
MES
|
Role: Buffer; Properties: Organic compound, Simple component, Defined component |
| 4 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 5 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
KNO3
K.NO3 101.102 g/mol
|
1 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 8 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 9 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.15 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Defined component, Inorganic compound, Simple component |
Solutions
Solution A
Dose: 1001 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 10 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Solution C
Dose: 10 G_PER_L
See mediadive_5312_Solution_C.yaml for composition
Synthetic seawater (2 x) (see Medium [M236])
Dose: 250 G_PER_L
Cross-reference to Medium M236
Wolfe's mineral elixir (see Medium [M471])
Dose: 1 G_PER_L
Cross-reference to Medium M471
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.916287Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M895 |
| 2026-01-28T19:48:22.857096Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:38.032540+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T03:09:07.786329Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:12.919041Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010312 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:14.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.864779+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Pyrolobus Medium" to "pyrolobus_medium" for consistent matching |
| 2026-05-16T09:03:21.813955+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:45.454095+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:51.970890+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |