knh_medium

CultureMech:010343 bacterial COMPLEX LIQUID
Original name: KNH Medium

Overview

External ontology
TOGO:M922 — KNH Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M922 Original source: JCM - JCM_M881 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=881

Composition

flowchart LR
    CultureMech_010343["knh_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010343 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_010343 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_010343 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_010343 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_NH4Cl["NH4Cl"]
    CultureMech_010343 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_Resazurin["Resazurin"]
    CultureMech_010343 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_010343 --> ing6_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_6H2O -.-> CHEBI_86345
    ing7_NaHCO3["NaHCO3"]
    CultureMech_010343 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_Sodium_acetate["Sodium acetate"]
    CultureMech_010343 --> ing8_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing8_Sodium_acetate -.-> CHEBI_32954
    ing9_Na2SO4["Na2SO4"]
    CultureMech_010343 --> ing9_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing9_Na2SO4 -.-> CHEBI_32149
    ing10_Ascorbic_acid["Ascorbic acid"]
    CultureMech_010343 --> ing10_Ascorbic_acid
    CHEBI_22652["CHEBI:22652"]
    ing10_Ascorbic_acid -.-> CHEBI_22652
    ing11_Sodium_thioglycolate["Sodium thioglycolate"]
    CultureMech_010343 --> ing11_Sodium_thioglycolate
    CHEBI_86481["CHEBI:86481"]
    ing11_Sodium_thioglycolate -.-> CHEBI_86481
    ing12_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_010343 --> ing12_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing12_Carbon_dioxide_gas -.-> CHEBI_16526
    ing13_Nitrogen_gas["Nitrogen gas"]
    CultureMech_010343 --> ing13_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing13_Nitrogen_gas -.-> CHEBI_17997
    ing14_Hydrogen_gas["Hydrogen gas"]
    CultureMech_010343 --> ing14_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing14_Hydrogen_gas -.-> CHEBI_18276

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 NaCl
Cl.Na 58.443 g/mol
20.45 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.147 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
4 KH2PO4
H2O4P.K 136.084 g/mol
0.136 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
5 NH4Cl
Cl.H4N 53.492 g/mol
0.535 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
6 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component
7 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
3.05 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
8 NaHCO3
CHO3.Na 84.006 g/mol
2.52 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
9 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.656 G_PER_L CHEBI:32954
sodium acetate
 Role: Buffer; Properties: Organic compound, Simple component, Defined component
10 Na2SO4
2Na.O4S 142.043 g/mol
2.84 G_PER_L CHEBI:32149
sodium sulfate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
11 Ascorbic acid 0.1 G_PER_L CHEBI:22652
ascorbic acid
 Role: Vitamin; Properties: Defined component, Organic compound, Simple component
12 Sodium thioglycolate
C2H3O2S.Na 114.101 g/mol
0.1 G_PER_L CHEBI:86481
sodium thioglycolate
 Properties: Defined component, Organic compound, Simple component
13 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
14 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
15 Hydrogen gas
H2 2.016 g/mol
variable VARIABLE CHEBI:18276
dihydrogen
 Properties: ガス

Solutions

Trace element solution (see Medium [M235])

Dose: 1 G_PER_L

Cross-reference to Medium M235

Se/W solution (see Medium [M278])

Dose: 1 G_PER_L

Cross-reference to Medium M278

Trace vitamins (see Medium [M190])

Dose: 1 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.965406Z togo-import Imported from TOGO Medium Source: JCM, ID: M922
2026-01-28T19:48:24.647456Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas
2026-03-09T08:04:40.757775+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T06:20:13.139184Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010343
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:17.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:32.120563+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "KNH Medium" to "knh_medium" for consistent matching
2026-05-16T09:03:22.037863+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:45.710683+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.053432+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.