Overview
- External ontology
- TOGO:M925 — Hyphomicrobium Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M925 Original source: JCM - JCM_M884 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=884
Composition
flowchart LR
CultureMech_010346["hyphomicrobium_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010346 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010346 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_K2HPO4["K2HPO4"]
CultureMech_010346 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_010346 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_Methylamine_hydrochloride["Methylamine hydrochloride"]
CultureMech_010346 --> ing4_Methylamine_hydrochloride
CHEBI_59337["CHEBI:59337"]
ing4_Methylamine_hydrochloride -.-> CHEBI_59337
ing5_NaH2PO4["NaH2PO4"]
CultureMech_010346 --> ing5_NaH2PO4
CHEBI_37585["CHEBI:37585"]
ing5_NaH2PO4 -.-> CHEBI_37585
ing6_Agar["Agar"]
CultureMech_010346 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010346 --> ing7_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_2H2O -.-> CHEBI_86158
ing8_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_010346 --> ing8_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_7H2O -.-> CHEBI_75836
ing9_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_010346 --> ing9_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing9_MnCl2_4H2O -.-> CHEBI_86368
ing10_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_010346 --> ing10_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing10_CoCl2_6H2O -.-> CHEBI_35696
ing11_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_010346 --> ing11_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing11_ZnSO4_7H2O -.-> CHEBI_32312
ing12_CuSO4_5H2O["CuSO4・5H2O"]
CultureMech_010346 --> ing12_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing12_CuSO4_5H2O -.-> CHEBI_31440
ing13__NH4_6Mo7O24_4H2O["(NH4)6Mo7O24・4H2O"]
CultureMech_010346 --> ing13__NH4_6Mo7O24_4H2O
CHEBI_91249["CHEBI:91249"]
ing13__NH4_6Mo7O24_4H2O -.-> CHEBI_91249
ing14_EDTA_2Na["EDTA・2Na"]
CultureMech_010346 --> ing14_EDTA_2Na
CHEBI_64734["CHEBI:64734"]
ing14_EDTA_2Na -.-> CHEBI_64734
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
501.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 500.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Methylamine hydrochloride
CH6N.Cl 67.519 g/mol
|
2 G_PER_L |
CHEBI:59337
methylamine hydrochloride
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 6 |
NaH2PO4
H2O4P.Na 119.976 g/mol
|
2.25 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 8 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.733 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.499 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.506 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
0.161 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
2.2 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.157 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
(NH4)6Mo7O24・4H2O
2H4N.MoO4 196.014 g/mol
|
0.11 G_PER_L |
CHEBI:91249
ammonium molybdate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
EDTA・2Na
C10H14N2O8.2Na 336.208 g/mol
|
5 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
Solutions
Visniac trace elements (see below)
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.972518Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M925 |
| 2026-01-28T19:48:33.879801Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:56.218688+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:27.774856Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:13.170420Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010346 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:37.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.150219+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Hyphomicrobium Medium" to "hyphomicrobium_medium" for consistent matching |
| 2026-05-16T09:03:22.073667+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:45.746151+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:33.993491+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:52.069536+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |