Overview
- External ontology
- TOGO:M926 — Phosphate-Buffered Saline (pH 7.4)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M926 Original source: JCM - JCM_M885 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=885
Composition
flowchart LR
CultureMech_010347["phosphate_buffered_saline_ph_7_4"]
ing0_Distilled_water["Distilled water"]
CultureMech_010347 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_010347 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_KH2PO4["KH2PO4"]
CultureMech_010347 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_KCl["KCl"]
CultureMech_010347 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_Na2HPO4_12H2O["Na2HPO4・12H2O"]
CultureMech_010347 --> ing4_Na2HPO4_12H2O
CHEBI_34683["CHEBI:34683"]
ing4_Na2HPO4_12H2O -.-> CHEBI_34683
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
8 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.2 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
Na2HPO4・12H2O
HO4P.2Na 141.958 g/mol
|
2.9 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.974677Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M926 |
| 2026-01-28T19:48:41.427731Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:13.176320Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010347 |
| 2026-03-17T06:30:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.156316+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Phosphate-Buffered Saline (pH 7.4)" to "phosphate_buffered_saline_ph_7_4" for consistent matching |
| 2026-06-05T07:40:45.753335+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.074577+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |