alkaline_nutrient_agar

CultureMech:010351 bacterial COMPLEX SOLID_AGAR
Original name: Alkaline Nutrient Agar

Overview

External ontology
TOGO:M92 — Alkaline Nutrient Agar
Applications
  • Microbial cultivation
pH
10.0
Notes
JCM Medium 100 / Alkaline Nutrient Agar uses the same Nutrient Agar basal formulation as JCM Medium 74, then after autoclaving adjusts the final medium to pH 10.0 with sterile 10% Na2CO3 solution. TOGO M92 and MediaDive J100 report the same JCM recipe.

Composition

flowchart LR
    CultureMech_010351["alkaline_nutrient_agar"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010351 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Peptone["Peptone"]
    CultureMech_010351 --> ing1_Peptone
    ing2_Beef_extract["Beef extract"]
    CultureMech_010351 --> ing2_Beef_extract
    ing3_Agar["Agar"]
    CultureMech_010351 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 TOGO M92 / JCM Medium 100 lists 1.0 L Distilled water.
2 Peptone 5.0 G_PER_L MICRO:0000178 (source link unavailable)
Peptone
 TOGO M92 / JCM Medium 100 lists 5.0 g/L Peptone.
3 Beef extract 3.0 G_PER_L FOODON:03302088
Beef extract
 TOGO M92 / JCM Medium 100 lists 3.0 g/L Beef extract.
4 Agar 15.0 G_PER_L CHEBI:2509
agar
 TOGO M92 / JCM Medium 100 lists 15.0 g/L Agar.

Preparation

  1. Dissolve 5.0 g/L peptone, 3.0 g/L beef extract, and 15.0 g/L agar in 1.0 L distilled water.
  2. Autoclave the basal nutrient agar.
  3. After autoclaving, adjust pH to 10.0 with sterile 10% Na2CO3 solution.

Solutions

10% Na2CO3 solution

Dose: variable VARIABLE

Stock composition
IngredientConcentrationNotes
Na2CO3 10.0 PERCENT_W_V TOGO M92 / JCM Medium 100 specifies the pH-adjusting sodium carbonate solution as 10.0% w/v.
TOGO M92 / JCM Medium 100 uses sterile 10% Na2CO3 solution to adjust the final medium to pH 10.0; the source does not specify a fixed addition volume.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.962240Z togo-import Imported from TOGO Medium Source: JCM, ID: M92
2026-01-28T19:48:14.309533Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Na2CO3 solution
2026-03-09T08:04:23.788768+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:13.201979Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010351
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:56.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:32.181785+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Alkaline Nutrient Agar" to "alkaline_nutrient_agar" for consistent matching
2026-05-16T09:03:22.105586+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:45.783262+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m92_alkaline_nutrient_score15.py RESOLVED_TOGO_M92_ALKALINE_NUTRIENT_AGAR Corrected water from 1 g/L to 1.0 L, grounded peptone and beef extract, replaced the empty Na2CO3 solution stub with a sterile 10% variable pH-adjuster stock, and linked the MediaDive J100 source duplicate.