5_salt_water_growth_medium (CultureMech:010360)

Overview

External ontology

TOGO:M938 — 5% Salt Water Growth Medium

Applications

Microbial cultivation

pH

7.5

Notes

TOGO M938 records JCM_M897 5% Salt Water Growth Medium with 500 ml distilled water, 0.6 g Yeast extract (BD-Difco), 3.0 g Peptone (Oxoid), 100.0 ml MDS salt water from TOGO M578/JCM Medium 574, and final adjustment to pH 7.5 with 1 M Tris base.

Composition

flowchart LR
    CultureMech_010360["5_salt_water_growth_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010360 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_010360 --> ing1_Yeast_extract__BD_Difco_
    ing2_Peptone__Oxoid_["Peptone (Oxoid)"]
    CultureMech_010360 --> ing2_Peptone__Oxoid_

Ingredients (3)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	500.0 ML_PER_L	CHEBI:15377 water	JCM Medium 897 lists 500.0 ml Distilled water.
2	Yeast extract (BD-Difco)	0.6 G_PER_L	FOODON:03315426 yeast extract	JCM Medium 897 lists 0.6 g Yeast extract (BD-Difco).
3	Peptone (Oxoid)	3.0 G_PER_L	MICRO:0000178 (source link unavailable) peptone	JCM Medium 897 lists 3.0 g Peptone (Oxoid).

Preparation

Mix distilled water, Yeast extract (BD-Difco), Peptone (Oxoid), and MDS salt water.
Adjust to pH 7.5 with 1 M Tris base.

Solutions

MDS salt water

Dose: 100.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
NaCl	240.0 G_PER_L	JCM Medium 574 lists 240.0 g NaCl in MDS salt water.
MgCl2 x 6H2O	30.0 G_PER_L	JCM Medium 574 lists 30.0 g MgCl2 x 6H2O in MDS salt water.
MgSO4 x 7H2O	35.0 G_PER_L	JCM Medium 574 lists 35.0 g MgSO4 x 7H2O in MDS salt water.
KCl	7.0 G_PER_L	JCM Medium 574 lists 7.0 g KCl in MDS salt water.
1 M CaCl2 solution	5.0 ML_PER_L	JCM Medium 574 adds 5 ml 1 M CaCl2 solution to MDS salt water.
Distilled water	1.0 L	JCM Medium 574 lists 1.0 L Distilled water in MDS salt water.

JCM Medium 897 adds 100.0 ml MDS salt water from TOGO M578/JCM 574.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:55.994036Z	togo-import	Imported from TOGO Medium	Source: JCM, ID: M938
2026-01-28T19:48:16.339601Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: Tris base
2026-03-09T08:04:26.968063+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 1 solution(s) from ingredients
2026-03-13T06:20:13.259357Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:010360
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name
2026-03-17T06:30:00.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:32.238153+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "5% Salt Water Growth Medium" to "5_salt_water_growth_medium" for consistent matching
2026-05-16T09:03:22.160119+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:40:45.844875+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00	repair_togo_m938_score15.py	RESOLVED_TOGO_M938_MDS_SALT_WATER	TOGO M938 records JCM_M897 5% Salt Water Growth Medium with 500 ml distilled water, 0.6 g Yeast extract (BD-Difco), 3.0 g Peptone (Oxoid), 100.0 ml MDS salt water from TOGO M578/JCM Medium 574, and final adjustment to pH 7.5 with 1 M Tris base.