bacillus_acidocaldarius_medium

CultureMech:010362 bacterial COMPLEX SOLID_AGAR
Original name: Bacillus Acidocaldarius Medium

Overview

External ontology
TOGO:M93 — Bacillus Acidocaldarius Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M93 Original source: JCM - JCM_M101 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=101

Composition

flowchart LR
    CultureMech_010362["bacillus_acidocaldarius_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010362 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_010362 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_010362 --> ing2_Yeast_extract
    ing3_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_010362 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_KH2PO4["KH2PO4"]
    CultureMech_010362 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5__NH4_2SO4["(NH4)2SO4"]
    CultureMech_010362 --> ing5__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing5__NH4_2SO4 -.-> CHEBI_62946
    ing6_Glucose["Glucose"]
    CultureMech_010362 --> ing6_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing6_Glucose -.-> CHEBI_17234
    ing7_Agar["Agar"]
    CultureMech_010362 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
500.0 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Collapsed 2 identical duplicates: 500.0, 500.0]
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 Yeast extract 1 G_PER_L Role: Nutrient source; Properties: Undefined component
4 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.25 G_PER_L CHEBI:86158
calcium chloride dihydrate
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
5 KH2PO4
H2O4P.K 136.084 g/mol
0.6 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
6 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.2 G_PER_L CHEBI:62946
ammonium sulfate
Role: Nitrogen source; Properties: Simple component, Defined component, Inorganic compound
7 Glucose
C6H12O6 180.156 g/mol
1 G_PER_L CHEBI:17234
glucose
Role: Carbon source; Properties: Defined component, Simple component, Organic compound
8 Agar 20 G_PER_L CHEBI:2509
agar
Role: Solidifying component

Solutions

Solution A

Dose: 500 G_PER_L

See mediadive_5342_Solution_A.yaml for composition

Solution B

Dose: 500 G_PER_L

See mediadive_5343_Solution_B.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.980522Z togo-import Imported from TOGO Medium Source: JCM, ID: M93
2026-01-28T19:48:16.276934Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:26.861685+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T03:08:55.530058Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:20:13.274750Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010362
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:29:59.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:32.253794+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Bacillus Acidocaldarius Medium" to "bacillus_acidocaldarius_medium" for consistent matching
2026-05-16T09:03:22.176982+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:45.863980+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:34.083626+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:52.103659+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-02T07:37:53.887856+00:00 repair_merged_duplicates.py REPAIRED_SUMMED_DUPLICATE_MERGE Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched.