Overview
- External ontology
- TOGO:M943 — MJ Medium For Microaerophilic Autotrophs
- Applications
-
- Microbial cultivation
- pH
- 5.5
- Notes
- JCM Medium 902, MediaDive J902, and TOGO M943 describe MJ Medium for Microaerophilic Autotrophs as 0.25 g/L NH4Cl in 1.0 L MJ(-N) synthetic seawater from TOGO M260/JCM Medium 268; after pH 5.5 adjustment, autoclaving, and cooling, 5.0 ml/L 8% NaHCO3 solution, 15.0 ml/L 10% Na2S2O3 x 5 H2O solution, and 10.0 ml/L Trace vitamins from TOGO M190/JCM Medium 197 are added aseptically and the medium is sealed under an N2-CO2-O2 (76:19:5, v/v) gas mixture at 50 kPa.
Composition
flowchart LR
CultureMech_010366["mj_medium_for_microaerophilic_autotrophs"]
ing0_NH4Cl["NH4Cl"]
CultureMech_010366 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_010366 --> ing1_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing1_Carbon_dioxide_gas -.-> CHEBI_16526
ing2_Nitrogen_gas["Nitrogen gas"]
CultureMech_010366 --> ing2_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing2_Nitrogen_gas -.-> CHEBI_17997
ing3_Oxygen_gas["Oxygen gas"]
CultureMech_010366 --> ing3_Oxygen_gas
CHEBI_15379["CHEBI:15379"]
ing3_Oxygen_gas -.-> CHEBI_15379
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
TOGO M943 / JCM Medium 902 lists 0.25 g/L NH4Cl. |
| 2 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
TOGO M943 / JCM Medium 902 uses Carbon dioxide gas in an N2-CO2-O2 (76:19:5, v/v) gas mixture. |
| 3 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
TOGO M943 / JCM Medium 902 uses Nitrogen gas in an N2-CO2-O2 (76:19:5, v/v) gas mixture. |
| 4 |
Oxygen gas
O2 31.998 g/mol
|
variable VARIABLE |
CHEBI:15379
dioxygen
|
TOGO M943 / JCM Medium 902 uses Oxygen gas in an N2-CO2-O2 (76:19:5, v/v) gas mixture. |
Preparation
- Mix NH4Cl with 1.0 L MJ(-N) synthetic seawater.
- Adjust to pH 5.5 and autoclave.
- After cooling, aseptically add filter-sterilized 5.0 ml/L 8% NaHCO3 solution, 15.0 ml/L 10% Na2S2O3 x 5 H2O solution, and 10.0 ml/L Trace vitamins.
- Distribute under an N2-CO2-O2 (76:19:5, v/v) gas mixture, seal with butyl rubber stoppers, and pressurize to 50 kPa with the same gas mixture.
Solutions
MJ(-N) synthetic seawater
Dose: 1000.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaCl | 30.0 G_PER_L | TOGO M260 / JCM Medium 268 lists 30.0 g/L NaCl. |
| K2HPO4 | 0.14 G_PER_L | TOGO M260 / JCM Medium 268 lists 0.14 g/L K2HPO4. |
| CaCl2 x 2 H2O | 0.14 G_PER_L | TOGO M260 / JCM Medium 268 lists 0.14 g/L CaCl2 x 2 H2O. |
| MgSO4 x 7 H2O | 3.4 G_PER_L | TOGO M260 / JCM Medium 268 lists 3.4 g/L MgSO4 x 7 H2O. |
| MgCl2 x 6 H2O | 4.18 G_PER_L | TOGO M260 / JCM Medium 268 lists 4.18 g/L MgCl2 x 6 H2O. |
| KCl | 0.33 G_PER_L | TOGO M260 / JCM Medium 268 lists 0.33 g/L KCl. |
| NiCl2 x 6 H2O | 0.5 MG_PER_L | TOGO M260 / JCM Medium 268 lists 0.5 mg/L NiCl2 x 6 H2O. |
| Na2SeO3 x 5 H2O | 0.5 MG_PER_L | TOGO M260 / JCM Medium 268 lists 0.5 mg/L Na2SeO3 x 5 H2O. |
| Fe(NH4)2(SO4)2 x 6 H2O | 0.01 G_PER_L | TOGO M260 / JCM Medium 268 lists 0.01 g/L Fe(NH4)2(SO4)2 x 6 H2O. |
| Trace minerals | 10.0 ML_PER_L | TOGO M260/JCM Medium 268 adds 10.0 ml/L Trace minerals from TOGO M142. |
| Distilled water | 1.0 L | TOGO M260/JCM Medium 268 lists 1.0 L Distilled water. |
TOGO M943 / JCM Medium 902 adds 1.0 L MJ(-N) synthetic seawater from TOGO M260/JCM Medium 268.
8% NaHCO3 solution
Dose: 5.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaHCO3 | 8.0 PERCENT_W_V | TOGO M943 / JCM Medium 902 specifies the added NaHCO3 solution as 8% w/v. |
TOGO M943 / JCM Medium 902 adds 5.0 ml/L 8% NaHCO3 solution after cooling.
10% Na2S2O3 x 5 H2O solution
Dose: 15.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2S2O3 x 5 H2O | 10.0 PERCENT_W_V | TOGO M943 / JCM Medium 902 specifies the added Na2S2O3 x 5 H2O solution as 10% w/v. |
TOGO M943 / JCM Medium 902 adds 15.0 ml/L 10% Na2S2O3 x 5 H2O solution after cooling.
Trace vitamins
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 2.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 2.0 mg/L Biotin. |
| Folic acid | 2.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 2.0 mg/L Folic acid. |
| Pyridoxine hydrochloride | 10.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 10.0 mg/L Pyridoxine hydrochloride. |
| Thiamine HCl | 5.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 5.0 mg/L Thiamine HCl. |
| Riboflavin | 5.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 5.0 mg/L Riboflavin. |
| Nicotinic acid | 5.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 5.0 mg/L Nicotinic acid. |
| Calcium pantothenate | 5.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 5.0 mg/L Calcium pantothenate. |
| Vitamin B12 | 0.1 MG_PER_L | TOGO M190 / JCM Medium 197 lists 0.1 mg/L Vitamin B12. |
| p-Aminobenzoic acid | 5.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 5.0 mg/L p-Aminobenzoic acid. |
| Lipoic acid | 5.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 5.0 mg/L Lipoic acid. |
| Distilled water | 1000.0 ML_PER_L | TOGO M190 / JCM Medium 197 lists 1000.0 ml/L Distilled water. |
TOGO M943 / JCM Medium 902 adds 10.0 ml/L Trace vitamins from TOGO M190/JCM Medium 197 after cooling.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.007052Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M943 |
| 2026-01-28T19:48:08.830588Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Oxygen gas |
| 2026-03-09T08:04:14.220682+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T06:20:13.315075Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010366 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.294311+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MJ Medium For Microaerophilic Autotrophs" to "mj_medium_for_microaerophilic_autotrophs" for consistent matching |
| 2026-05-16T09:03:22.220802+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:45.913959+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.114682+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m943_microaerophilic_autotrophs_score15.py | RESOLVED_TOGO_M943_MICROAEROPHILIC_AUTOTROPHS | Moved MJ(-N) synthetic seawater, 8% NaHCO3, 10% Na2S2O3 x 5 H2O, and Trace vitamins from empty solution stubs into structured solution entries, corrected their ml/L addition volumes, added pH 5.5 and microaerophilic gas handling, grounded Oxygen gas, and removed the stale MediaDive J780 match. |