Overview
- External ontology
- TOGO:M947 — 225M1 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M947 Original source: JCM - JCM_M905 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=905
Composition
flowchart LR
CultureMech_010370["225m1_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010370 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_010370 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010370 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010370 --> ing3_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_6H2O -.-> CHEBI_86345
ing4_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_010370 --> ing4_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing4_Sodium_pyruvate -.-> CHEBI_50144
ing5_K2SO4["K2SO4"]
CultureMech_010370 --> ing5_K2SO4
CHEBI_32036["CHEBI:32036"]
ing5_K2SO4 -.-> CHEBI_32036
ing6_Soluble_starch["Soluble starch"]
CultureMech_010370 --> ing6_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing6_Soluble_starch -.-> CHEBI_28017
ing7_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010370 --> ing7_Yeast_extract__BD_Difco_
ing8_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
CultureMech_010370 --> ing8_Casamino_acids__BD_Difco_
ing9_KOH["KOH"]
CultureMech_010370 --> ing9_KOH
CHEBI_32035["CHEBI:32035"]
ing9_KOH -.-> CHEBI_32035
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.13 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
300 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 5 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.13 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 6 |
K2SO4
2K.O4S 174.259 g/mol
|
5 G_PER_L |
CHEBI:32036
potassium sulfate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 7 | Soluble starch | 2 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 8 | Yeast extract (BD-Difco) | 1 G_PER_L | Properties: Undefined component | |
| 9 | Casamino acids (BD-Difco) | 1 G_PER_L | Properties: Undefined component | |
| 10 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.014427Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M947 |
| 2026-01-28T19:48:32.997057Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH |
| 2026-03-13T06:20:13.345539Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010370 |
| 2026-03-17T06:30:35.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.323845+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "225M1 Medium" to "225m1_medium" for consistent matching |
| 2026-05-16T09:03:22.250386+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:45.949855+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.125011+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |