Overview
- External ontology
- TOGO:M94 — Nutrient Agar With 25% Soil Extract
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- JCM Medium 102 Nutrient Agar with 25% Soil Extract lists, per liter, 5.0 g Peptone, 3.0 g Beef extract, 250 ml Soil extract, 15.0 g Agar, and 750 ml Tap water, with final pH adjusted to 7.0. Soil extract is prepared by suspending 400 g air-dried garden soil in 1.0 L tap water, autoclaving at 121 C for 1 hr, then using the clear supernatant after a few hours of sedimentation and centrifugation.
Composition
flowchart LR
CultureMech_010373["nutrient_agar_with_25_soil_extract"]
ing0_Peptone["Peptone"]
CultureMech_010373 --> ing0_Peptone
ing1_Beef_extract["Beef extract"]
CultureMech_010373 --> ing1_Beef_extract
ing2_Tap_water["Tap water"]
CultureMech_010373 --> ing2_Tap_water
CHEBI_15377["CHEBI:15377"]
ing2_Tap_water -.-> CHEBI_15377
ing3_Agar["Agar"]
CultureMech_010373 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 5.0 G_PER_L |
MICRO:0000178 (source link unavailable)
Peptone
|
TOGO M94 / JCM Medium 102 lists 5.0 g/L Peptone. |
| 2 | Beef extract | 3.0 G_PER_L |
FOODON:03302088
Beef extract
|
TOGO M94 / JCM Medium 102 lists 3.0 g/L Beef extract. |
| 3 |
Tap water
H2O 18.015 g/mol
|
750.0 ML_PER_L |
CHEBI:15377
water
|
TOGO M94 / JCM Medium 102 lists 750.0 ml/L Tap water. |
| 4 | Agar | 15.0 G_PER_L |
CHEBI:2509
agar
|
TOGO M94 / JCM Medium 102 lists 15.0 g/L Agar. |
Preparation
- Suspend 400 g air-dried garden soil in 1.0 L tap water and autoclave at 121 C for 1 hr to prepare Soil extract.
- Let the soil slurry sediment for a few hours, then centrifuge and use the clear supernatant as Soil extract.
- Prepare the main liter with 5.0 g Peptone, 3.0 g Beef extract, 250 ml Soil extract, 15.0 g Agar, and 750 ml Tap water.
- Adjust pH to 7.0.
Solutions
Soil extract
Dose: 250.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Air-dried garden soil | 400.0 G_PER_L | TOGO M94 / JCM Medium 102 prepares Soil extract from 400 g air-dried garden soil in 1.0 L Tap water. |
| Tap water | 1.0 L | TOGO M94 / JCM Medium 102 prepares Soil extract with 1.0 L Tap water. |
TOGO M94 / JCM Medium 102 adds 250 ml/L Soil extract to the main liter.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.997509Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M94 |
| 2026-01-28T19:48:42.652658Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:43.726638Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:13.364048Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010373 |
| 2026-03-17T06:30:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.342150+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Nutrient Agar With 25% Soil Extract" to "nutrient_agar_with_25_soil_extract" for consistent matching |
| 2026-06-05T07:40:45.971893+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m94_soil_extract_score15.py | RESOLVED_TOGO_M94_SOIL_EXTRACT | Corrected the imported water merge and ml-to-g/L artifacts, moved Soil extract to a 250 ml/L nested solution, added pH 7.0, and grounded Peptone and Beef extract to complex-source terms. |