Overview
- External ontology
- TOGO:M951 — Geobacter Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M951 Original source: JCM - JCM_M907 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=907
Composition
flowchart LR
CultureMech_010375["geobacter_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010375 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_010375 --> ing1_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_2H2O -.-> CHEBI_86158
ing2_KH2PO4["KH2PO4"]
CultureMech_010375 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NH4Cl["NH4Cl"]
CultureMech_010375 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_010375 --> ing4_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_6H2O -.-> CHEBI_86345
ing5_Sodium_acetate["Sodium acetate"]
CultureMech_010375 --> ing5_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing5_Sodium_acetate -.-> CHEBI_32954
ing6_Na2SO4["Na2SO4"]
CultureMech_010375 --> ing6_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing6_Na2SO4 -.-> CHEBI_32149
ing7_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_010375 --> ing7_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing7_Carbon_dioxide_gas -.-> CHEBI_16526
ing8_Nitrogen_gas["Nitrogen gas"]
CultureMech_010375 --> ing8_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing8_Nitrogen_gas -.-> CHEBI_17997
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
910 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.147 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.136 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.535 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.204 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.82 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Defined component, Simple component, Organic compound |
| 7 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.142 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 8 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 9 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Solutions
Trace element solution (see Medium [M235])
Dose: 10 G_PER_L
Cross-reference to Medium M235
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Se/W solution (see Medium [M278])
Dose: 1 G_PER_L
Cross-reference to Medium M278
8% NaHCO3 solution*
Dose: 30 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
1.0 M Sodium fumarate solution
Dose: 10 G_PER_L
Properties: Defined component, Organic compound, Solution
Iron(III) citrate solution (see Medium [M1072])
Dose: 50 G_PER_L
Cross-reference to Medium M1072
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.022012Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M951 |
| 2026-01-28T19:48:42.103922Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:05:10.981775+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T06:20:13.379398Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010375 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.357165+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Geobacter Medium" to "geobacter_medium" for consistent matching |
| 2026-05-16T09:03:22.286444+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:45.989036+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.142471+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |