Overview
- External ontology
- TOGO:M961 — Semi-Solid Autotrophic Growth Medium For Magnetococcus Marinus
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M961 Original source: JCM - JCM_M915 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=915
Composition
flowchart LR
CultureMech_010386["semi_solid_autotrophic_growth_medium_for_magnetococcus_marinus"]
ing0_Distilled_water["Distilled water"]
CultureMech_010386 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_010386 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_010386 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_NH4Cl["NH4Cl"]
CultureMech_010386 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_Resazurin["Resazurin"]
CultureMech_010386 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_010386 --> ing5_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_6H2O -.-> CHEBI_86345
ing6_KCl["KCl"]
CultureMech_010386 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_Na2SO4["Na2SO4"]
CultureMech_010386 --> ing7_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing7_Na2SO4 -.-> CHEBI_32149
ing8_Agarose["Agarose"]
CultureMech_010386 --> ing8_Agarose
CHEBI_2511["CHEBI:2511"]
ing8_Agarose -.-> CHEBI_2511
ing9_N2["N2"]
CultureMech_010386 --> ing9_N2
CHEBI_17997["CHEBI:17997"]
ing9_N2 -.-> CHEBI_17997
ing10_Air["Air"]
CultureMech_010386 --> ing10_Air
ing11_0_5_M_Potassium_phosphate_buffer__pH_7_0_["0.5 M Potassium phosphate buffer (pH 7.0)"]
CultureMech_010386 --> ing11_0_5_M_Potassium_phosphate_buffer__pH_7_0_
ing12_HCl["HCl"]
CultureMech_010386 --> ing12_HCl
CHEBI_17883["CHEBI:17883"]
ing12_HCl -.-> CHEBI_17883
ing13_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_010386 --> ing13_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing13_MgSO4_7H2O -.-> CHEBI_31795
ing14_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_010386 --> ing14_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_2H2O -.-> CHEBI_75213
ing15_H3BO3["H3BO3"]
CultureMech_010386 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_010386 --> ing16_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing16_FeSO4_7H2O -.-> CHEBI_75836
ing17_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_010386 --> ing17_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_6H2O -.-> CHEBI_34887
ing18_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_010386 --> ing18_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing18_ZnSO4_7H2O -.-> CHEBI_32312
ing19_CuSO4_5H2O["CuSO4·5H2O"]
CultureMech_010386 --> ing19_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing19_CuSO4_5H2O -.-> CHEBI_31440
ing20_CoSO4_7H2O["CoSO4·7H2O"]
CultureMech_010386 --> ing20_CoSO4_7H2O
CHEBI_91244["CHEBI:91244"]
ing20_CoSO4_7H2O -.-> CHEBI_91244
ing21_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_010386 --> ing21_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing21_Nitrilotriacetic_acid -.-> CHEBI_44557
ing22_MnSO4_xH2O["MnSO4·xH2O"]
CultureMech_010386 --> ing22_MnSO4_xH2O
ing23_AlK_SO4_2["AlK(SO4)2"]
CultureMech_010386 --> ing23_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing23_AlK_SO4_2 -.-> CHEBI_86463
ing24_Biotin["Biotin"]
CultureMech_010386 --> ing24_Biotin
CHEBI_15956["CHEBI:15956"]
ing24_Biotin -.-> CHEBI_15956
ing25_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_010386 --> ing25_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing25_p_Aminobenzoic_acid -.-> CHEBI_30753
ing26_Thiamine_HCl["Thiamine·HCl"]
CultureMech_010386 --> ing26_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing26_Thiamine_HCl -.-> CHEBI_49105
ing27_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_010386 --> ing27_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing27_Calcium_pantothenate -.-> CHEBI_31345
ing28_Pyridoxine_HCl["Pyridoxine·HCl"]
CultureMech_010386 --> ing28_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing28_Pyridoxine_HCl -.-> CHEBI_30961
ing29_Folic_acid["Folic acid"]
CultureMech_010386 --> ing29_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing29_Folic_acid -.-> CHEBI_27470
CultureMech_010386 --> more["...3 more"]
Ingredients (33)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
102.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 1.0, 100.0] |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
17.4 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 16.4, 1.0] |
| 3 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.49 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.39, 0.1] |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.3 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.4 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 6 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3.49 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.47 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Na2SO4
2Na.O4S 142.043 g/mol
|
2.74 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Agarose
(C12H18O9)n.C12H20O10 630.549 g/mol
|
2 G_PER_L |
CHEBI:2511
agarose
|
Role: Solidifying component |
| 10 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 11 | Air | variable VARIABLE | Properties: ガス | |
| 12 | 0.5 M Potassium phosphate buffer (pH 7.0) | 1.8 G_PER_L | Role: Buffer; Properties: Solution, Defined component, Inorganic compound, Complex component | |
| 13 |
HCl
HCl 36.461 g/mol
|
variable VARIABLE |
CHEBI:17883
hydrogen chloride
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Solution |
| 14 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.4 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
0.01 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.02 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
CoSO4·7H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 23 | MnSO4·xH2O | 0.5 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 24 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.1 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 26 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 27 |
Thiamine·HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
90 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 28 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
4 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 29 |
Pyridoxine·HCl
C8H11NO3.HCl 205.641 g/mol
|
4 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 30 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 31 | Vitamin B12 | 5 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 32 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
4 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 33 |
myo-Inositol
C6H12O6 180.156 g/mol
|
40 G_PER_L |
CHEBI:17268
myo-inositol
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
Solutions
Modified Wolfe's mineral solution (see below)
Dose: 5 G_PER_L
8% NaHCO3 solution*
Dose: 2.35 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
40% Na2S2O3·5H2O solution
Dose: 3 G_PER_L
Properties: Defined component, Inorganic compound, Solution
5% L-Cysteine·HCl·H2O solution
Dose: 4 G_PER_L
Properties: Defined component, Solution, Organic compound
Vitamin solution* (see below)
Dose: 0.5 G_PER_L
0.01 M FeSO4·5H2Osolution (in 0.02 N HCl)
Dose: 2.5 G_PER_L
Role: Mineral source; Properties: Inorganic compound, Defined component, Solution
KOH solution
Dose: variable VARIABLE
Role: Mineral source; Properties: Defined component, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.038528Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M961 |
| 2026-01-28T19:48:26.661958Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2; Added default concentration for ingredient: Air; Added default concentration for ingredient: HCl; Added default concentration for ingredient: HCl; Added default concentration for ingredient: KOH solution |
| 2026-03-09T08:04:44.258255+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 7 solution(s) from ingredients |
| 2026-03-13T03:09:14.464180Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:20:13.462990Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010386 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.436728+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Semi-Solid Autotrophic Growth Medium For Magnetococcus Marinus" to "semi_solid_autotrophic_growth_medium_for_magnetococcus_marinus" for consistent matching |
| 2026-05-16T09:03:22.352045+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:46.067617+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:35.273692+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T06:57:34.252894+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:52.157258+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |