mrs_medium_with_soybean_peptone

CultureMech:010397 bacterial COMPLEX SOLID_AGAR
Original name: MRS Medium With Soybean Peptone

Overview

External ontology
TOGO:M971 — MRS Medium With Soybean Peptone
Applications
  • Microbial cultivation
Notes
JCM Medium 925 MRS Medium With Soybean Peptone lists 55.0 g/L Lactobacilli MRS broth, 5.0 g/L Phytone peptone, 15.0 g/L Bacto agar, and 1.0 L Distilled water; the JCM page applies default autoclaving at 121 C for 15 min.

Composition

flowchart LR
    CultureMech_010397["mrs_medium_with_soybean_peptone"]
    ing0_Lactobacilli_MRS_broth__BD_Difco_["Lactobacilli MRS broth (BD-Difco)"]
    CultureMech_010397 --> ing0_Lactobacilli_MRS_broth__BD_Difco_
    ing1_Phytone_peptone__BD_BBL_["Phytone peptone (BD-BBL)"]
    CultureMech_010397 --> ing1_Phytone_peptone__BD_BBL_
    ing2_Bacto_agar__BD_Difco_["Bacto agar (BD-Difco)"]
    CultureMech_010397 --> ing2_Bacto_agar__BD_Difco_
    CHEBI_2509["CHEBI:2509"]
    ing2_Bacto_agar__BD_Difco_ -.-> CHEBI_2509
    ing3_Distilled_water["Distilled water"]
    CultureMech_010397 --> ing3_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Distilled_water -.-> CHEBI_15377

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Lactobacilli MRS broth (BD-Difco) 55.0 G_PER_L TOGO M971 / JCM Medium 925 lists 55.0 g/L Lactobacilli MRS broth (BD-Difco).
2 Phytone peptone (BD-BBL) 5.0 G_PER_L FOODON:03315720
Soy peptone
 TOGO M971 / JCM Medium 925 lists 5.0 g/L Phytone peptone (BD-BBL).
3 Bacto agar (BD-Difco) 15.0 G_PER_L CHEBI:2509
agar
 TOGO M971 / JCM Medium 925 lists 15.0 g/L Bacto agar (BD-Difco).
4 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 TOGO M971 / JCM Medium 925 lists 1.0 L Distilled water.

Preparation

  1. Combine the MRS Medium With Soybean Peptone components listed by JCM Medium 925.
  2. Autoclave at 121 C for 15 min unless otherwise stated.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.059260Z togo-import Imported from TOGO Medium Source: JCM, ID: M971
2026-01-28T19:48:13.511246Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:13.543180Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010397
2026-03-17T06:29:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:32.516560+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MRS Medium With Soybean Peptone" to "mrs_medium_with_soybean_peptone" for consistent matching
2026-06-05T07:40:46.148865+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m971_mrs_soybean_score15.py RESOLVED_TOGO_M971_MRS_SOYBEAN Corrected the JCM Medium 925 water unit, grounded Bacto agar and Phytone peptone, retained Lactobacilli MRS broth as a source-listed unmapped commercial product, added the JCM default autoclaving condition, and linked the MediaDive J925 source duplicate.