Overview
- External ontology
- TOGO:M975 — Halanaerobium Sehlinense Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M975 Original source: JCM - JCM_M929 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=929
Ingredients (0)
No ingredients recorded.
Preparation
- Add components to distilled water and bring volume to 1.0 L. Adjust pH to 8.3 with 10 M KOH. Bring to a boil and cool down under a N2 gas stream.Then, distribute the medium into culture vessels (e.g., 5 ml of the mediumin the Hungate tubes) under a N2--CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave at 110C for 20 min. Aseptically and anaerobically add the following solutions (autoclaved or *filter--sterilized) into the medium (per liter):
Solutions
Basal preparation at the 1 L makeup stage
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component Water is the makeup reagent for the explicitly stated 1.0 L basal stage, not a fixed 1 L water input or a final supplemented-medium volume. Its actual addition amount is unspecified. The original table also lists 1.0 L water; this conflicts with treating that value as a fixed input to the separate 1.0 L makeup instruction. Both statements are preserved as source context; no numeric water dose is asserted. | |
| Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component Source basal batch amount: 1 g; explicitly made up to 1.0 L at this stage. Calculation: 1 g / 1.0 L = 1 g/L. This concentration applies at basal makeup, before subsequent pH adjustment, heating and supplements; it is not a final supplemented-medium rate. |
| NaCl | 200 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component Source basal batch amount: 200 g; explicitly made up to 1.0 L at this stage. Calculation: 200 g / 1.0 L = 200 g/L. This concentration applies at basal makeup, before subsequent pH adjustment, heating and supplements; it is not a final supplemented-medium rate. |
| CaCl2・2H2O | 0.1 G_PER_L | Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound Source basal batch amount: 0.1 g; explicitly made up to 1.0 L at this stage. Calculation: 0.1 g / 1.0 L = 0.1 g/L. This concentration applies at basal makeup, before subsequent pH adjustment, heating and supplements; it is not a final supplemented-medium rate. |
| KH2PO4 | 0.3 G_PER_L | Role: Buffer; Properties: Simple component, Inorganic compound, Defined component Source basal batch amount: 0.3 g; explicitly made up to 1.0 L at this stage. Calculation: 0.3 g / 1.0 L = 0.3 g/L. This concentration applies at basal makeup, before subsequent pH adjustment, heating and supplements; it is not a final supplemented-medium rate. |
| NH4Cl | 1 G_PER_L | Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component Source basal batch amount: 1 g; explicitly made up to 1.0 L at this stage. Calculation: 1 g / 1.0 L = 1 g/L. This concentration applies at basal makeup, before subsequent pH adjustment, heating and supplements; it is not a final supplemented-medium rate. |
| K2HPO4 | 0.3 G_PER_L | Role: Buffer; Properties: Simple component, Inorganic compound, Defined component Source basal batch amount: 0.3 g; explicitly made up to 1.0 L at this stage. Calculation: 0.3 g / 1.0 L = 0.3 g/L. This concentration applies at basal makeup, before subsequent pH adjustment, heating and supplements; it is not a final supplemented-medium rate. |
| KCl | 0.1 G_PER_L | Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound Source basal batch amount: 0.1 g; explicitly made up to 1.0 L at this stage. Calculation: 0.1 g / 1.0 L = 0.1 g/L. This concentration applies at basal makeup, before subsequent pH adjustment, heating and supplements; it is not a final supplemented-medium rate. |
| KNO3 | 2 G_PER_L | Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound Source basal batch amount: 2 g; explicitly made up to 1.0 L at this stage. Calculation: 2 g / 1.0 L = 2 g/L. This concentration applies at basal makeup, before subsequent pH adjustment, heating and supplements; it is not a final supplemented-medium rate. |
| Carbon dioxide gas | Properties: ガス Source atmosphere context, not a fixed gas input or dissolved concentration. Removed the unsupported VARIABLE placeholder. | |
| N2 | Properties: ガス Source atmosphere context, not a fixed gas input or dissolved concentration. Removed the unsupported VARIABLE placeholder. |
This is the explicitly made-up basal preparation before later supplements. Composition rates apply at the 1.0 L makeup stage only. No fixed dose of the subsequently titrated/heated basal preparation or final supplemented-medium volume is asserted.
Trace minerals (see Medium [M142])
Dose: 0.01 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 1 L | Source water batch input: 1 L = 1 L. This is not a measured final volume. |
| MgSO4・7H2O | Source batch input: 3 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. | |
| NaCl | Source batch input: 1 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. | |
| CaCl2・2H2O | Source batch input: 0.1 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. | |
| Na2MoO4・2H2O | Source batch input: 0.01 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. | |
| H3BO3 | Source batch input: 0.01 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. | |
| FeSO4・7H2O | Source batch input: 0.1 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. | |
| ZnSO4・7H2O | Source batch input: 0.1 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. | |
| CuSO4・5H2O | Source batch input: 0.01 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. | |
| CoSO4・7H2O | Source batch input: 0.1 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. | |
| Nitrilotriacetic acid | Source batch input: 1.5 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. | |
| MnSO4・xH2O | Source batch input: 0.5 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. | |
| AlK(SO4)2 | Source batch input: 0.01 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. |
Cross-reference to Medium M142
Source liquid input: 10 ml = 0.01 L per basal makeup batch, not grams per liter.
KOH solution
Stock pH-adjustment reagent with no fixed dose or numeric strength.
KOH reagent (10 M)
| Ingredient | Concentration | Notes |
|---|---|---|
| KOH | 10 MOLAR | Role: Mineral source; Properties: Defined component, Solution Use the source-explicit 10 M KOH reagent to adjust basal pH to 8.3. The addition volume is unspecified; 10 M describes the reagent, not the basal or final medium. |
Use the source-explicit 10 M KOH reagent to adjust basal pH to 8.3. The addition volume is unspecified; 10 M describes the reagent, not the basal or final medium.
8% NaHCO3 solution*
Dose: 25 ML_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Source-explicit post-autoclave addition: 25 ml per liter of receiving basal medium; not a final-mixture volume measurement. Stock label is 8%; its percentage basis is unspecified, so no numeric strength or final concentration is inferred.
10% MgCl2・6H2O solution
Dose: 15 ML_PER_L
Role: Mineral source; Properties: Inorganic compound, Solution, Defined component
Source-explicit post-autoclave addition: 15 ml per liter of receiving basal medium; not a final-mixture volume measurement. Stock label is 10%; its percentage basis is unspecified, so no numeric strength or final concentration is inferred.
1 M Glucose solution
Dose: 20 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Glucose | 1 MOLAR | Explicit 1 M stock strength, not a receiving-medium concentration. No final dilution is calculated. |
Role: Carbon source; Properties: Organic compound, Solution, Defined component
Source-explicit post-autoclave addition: 20 ml per liter of receiving basal medium; not a final-mixture volume measurement.
5% Na2S・9H2O solution
Dose: 8 ML_PER_L
Role: Reducing agent; Properties: Solution, Inorganic compound, Defined component
Source-explicit post-autoclave addition: 8 ml per liter of receiving basal medium; not a final-mixture volume measurement. Stock label is 5%; its percentage basis is unspecified, so no numeric strength or final concentration is inferred.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.068312Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M975 |
| 2026-01-28T19:48:25.408650Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:42.059166+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T06:20:13.587003Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010401 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:19.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.559067+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Halanaerobium Sehlinense Medium" to "halanaerobium_sehlinense_medium" for consistent matching |
| 2026-05-16T09:03:22.472573+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:46.198441+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.208149+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-08T17:42:06.870442+00:00 | codex | RESTORED_BASAL_MAKEUP_AND_SUPPLEMENT_STAGES_WITH_EVIDENCE | Restored explicit basal-makeup and later supplementation boundaries, scoped ingredient quantities and original preparation. Preserved chemical annotations and prior history; no final supplemented-medium volume or unspecified percentage basis is inferred. Source-defined M823/M853 variant links preserve the salt substitution and pH override. |