leibovitzs_l_15_medium_with_10_fbs_and_1_5_nacl

CultureMech:010402 bacterial COMPLEX LIQUID
Original name: Leibovitz's L-15 Medium With 10% FBS And 1.5% NaCl

Overview

External ontology
TOGO:M976 — Leibovitz's L-15 Medium With 10% FBS And 1.5% NaCl
Applications
  • Microbial cultivation
Notes
JCM Medium 930 lists 1.0 L Leibovitz's L-15 medium, 100.0 ml Fetal bovine serum, and 15.0 g NaCl; filter-sterilize with a 0.22 um PES filter.

Composition

flowchart LR
    CultureMech_010402["leibovitzs_l_15_medium_with_10_fbs_and_1_5_nacl"]
    ing0_Leibovitz_s_L_15_medium["Leibovitz's L-15 medium"]
    CultureMech_010402 --> ing0_Leibovitz_s_L_15_medium
    ing1_Fetal_bovine_serum["Fetal bovine serum"]
    CultureMech_010402 --> ing1_Fetal_bovine_serum
    ing2_NaCl["NaCl"]
    CultureMech_010402 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Leibovitz's L-15 medium 1.0 L mediadive.compound:1941 (source link unavailable)
Leibovitz's L-15 medium
 TOGO M976 / JCM Medium 930 lists 1.0 L Leibovitz's L-15 medium.
2 Fetal bovine serum 100.0 ML_PER_L mediadive.compound:954 (source link unavailable)
Fetal bovine serum
 TOGO M976 / JCM Medium 930 lists 100.0 ml/L Fetal bovine serum.
3 NaCl
Cl.Na 58.443 g/mol
15.0 G_PER_L CHEBI:26710
sodium chloride
 TOGO M976 / JCM Medium 930 lists 15.0 g/L NaCl.

Preparation

  1. Combine the JCM Medium 930 Leibovitz L-15, fetal bovine serum, and NaCl components.
  2. Filter-sterilize with a 0.22 um PES filter.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.070315Z togo-import Imported from TOGO Medium Source: JCM, ID: M976
2026-01-28T19:48:27.750396Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:13.592459Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010402
2026-03-17T06:30:24.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:32.565100+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Leibovitz's L-15 Medium With 10% FBS And 1.5% NaCl" to "leibovitzs_l_15_medium_with_10_fbs_and_1_5_nacl" for consistent matching
2026-06-05T07:40:46.204872+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m976_leibovitz_score15.py RESOLVED_TOGO_M976_LEIBOVITZ_FBS Corrected the TOGO M976 volume imports, fixed the L-15 medium name, grounded NaCl to CHEBI, linked the disclosed L-15 and FBS products to MediaDive compound IDs, and linked MediaDive J930 as the source duplicate parent.