Overview
- External ontology
- TOGO:M978 — Oscillibacter GH Medium
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- JCM Medium 932 lists 5.0 g yeast extract from BD-Difco, 5.0 g Polypeptone peptone from BD-BBL, 0.03 g Tween 80, 10.0 ml Trace vitamins from JCM Medium 197, 1.0 ml FeCl2 solution from JCM Medium 187, 1.0 ml Trace element solution from JCM Medium 187, and 1.0 L distilled water; after mixing, adjust pH to 6.0, distribute under N2, seal with butyl rubber stoppers, and autoclave.
Composition
flowchart LR
CultureMech_010404["oscillibacter_gh_medium"]
ing0_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010404 --> ing0_Yeast_extract__BD_Difco_
ing1_Polypeptone_peptone__BD_BBL_["Polypeptone peptone (BD-BBL)"]
CultureMech_010404 --> ing1_Polypeptone_peptone__BD_BBL_
ing2_Tween_80["Tween 80"]
CultureMech_010404 --> ing2_Tween_80
CHEBI_53426["CHEBI:53426"]
ing2_Tween_80 -.-> CHEBI_53426
ing3_Distilled_water["Distilled water"]
CultureMech_010404 --> ing3_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing3_Distilled_water -.-> CHEBI_15377
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract (BD-Difco) | 4.94071 G_PER_L |
FOODON:03315426
yeast extract
|
TOGO M978 / JCM Medium 932 lists 4.94071 g/L Yeast extract (BD-Difco). |
| 2 | Polypeptone peptone (BD-BBL) | 4.94071 G_PER_L |
FOODON:03315306
Polypeptone
|
TOGO M978 / JCM Medium 932 lists 4.94071 g/L Polypeptone peptone (BD-BBL). |
| 3 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
0.0296443 G_PER_L |
CHEBI:53426
polysorbate 80
|
TOGO M978 / JCM Medium 932 lists 0.0296443 g/L Tween 80. |
| 4 |
Distilled water
H2O 18.015 g/mol
|
988.142 ML_PER_L |
CHEBI:15377
water
|
TOGO M978 / JCM Medium 932 makes 1.012 L medium from 1.0 L distilled water plus 12.0 ml stock additions, equivalent to 988.142 ml/L distilled water. |
Preparation
- Mix yeast extract, Polypeptone peptone, Tween 80, distilled water, 9.88142 ml/L Trace vitamins, 0.988142 ml/L FeCl2 solution, and 0.988142 ml/L Trace element solution.
- Adjust pH to 6.0.
- Distribute the medium into culture vessels under a N2 gas stream and seal with butyl rubber stoppers.
- Autoclave the sealed medium.
Solutions
Trace vitamins
Dose: 9.88142 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 2.0 MG_PER_L | JCM Medium 197 lists 2.0 mg/L Biotin. |
| Folic acid | 2.0 MG_PER_L | JCM Medium 197 lists 2.0 mg/L Folic acid. |
| Pyridoxine hydrochloride | 10.0 MG_PER_L | JCM Medium 197 lists 10.0 mg/L Pyridoxine hydrochloride. |
| Thiamine HCl | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Thiamine HCl. |
| Riboflavin | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Riboflavin. |
| Nicotinic acid | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Nicotinic acid. |
| Calcium pantothenate | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Calcium pantothenate. |
| Vitamin B12 | 0.1 MG_PER_L | JCM Medium 197 lists 0.1 mg/L Vitamin B12. |
| p-Aminobenzoic acid | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L p-Aminobenzoic acid. |
| Lipoic acid | 5.0 MG_PER_L | JCM Medium 197 lists 5.0 mg/L Lipoic acid. |
| Distilled water | 1000.0 ML_PER_L | JCM Medium 197 lists 1000.0 ml/L Distilled water. |
TOGO M978 / JCM Medium 932 adds 9.88142 ml/L Trace vitamins.
FeCl2 solution
Dose: 0.988142 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| HCl | 2.5 G_PER_L | JCM Medium 187 lists 2.5 g/L HCl. |
| FeCl2 x 4 H2O | 1.5 G_PER_L | JCM Medium 187 lists 1.5 g/L FeCl2 x 4 H2O. |
| Distilled water | 990.0 ML_PER_L | JCM Medium 187 lists 990.0 ml/L Distilled water. |
TOGO M978 / JCM Medium 932 adds 0.988142 ml/L FeCl2 solution.
Trace element solution
Dose: 0.988142 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| ZnCl2 | 70.0 MG_PER_L | JCM Medium 187 lists 70.0 mg/L ZnCl2. |
| MnCl2 x 4 H2O | 100.0 MG_PER_L | JCM Medium 187 lists 100.0 mg/L MnCl2 x 4 H2O. |
| H3BO3 | 6.0 MG_PER_L | JCM Medium 187 lists 6.0 mg/L H3BO3. |
| CoCl2 x 6 H2O | 190.0 MG_PER_L | JCM Medium 187 lists 190.0 mg/L CoCl2 x 6 H2O. |
| CuCl2 x 2 H2O | 2.0 MG_PER_L | JCM Medium 187 lists 2.0 mg/L CuCl2 x 2 H2O. |
| NiCl2 x 6 H2O | 24.0 MG_PER_L | JCM Medium 187 lists 24.0 mg/L NiCl2 x 6 H2O. |
| Na2MoO4 x 2 H2O | 36.0 MG_PER_L | JCM Medium 187 lists 36.0 mg/L Na2MoO4 x 2 H2O. |
| Distilled water | 1000.0 ML_PER_L | JCM Medium 187 lists 1000.0 ml/L Distilled water. |
TOGO M978 / JCM Medium 932 adds 0.988142 ml/L Trace element solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.072024Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M978 |
| 2026-01-28T19:48:09.704380Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:15.744400+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T06:20:13.600590Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010404 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.573960+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Oscillibacter GH Medium" to "oscillibacter_gh_medium" for consistent matching |
| 2026-05-16T09:03:22.484861+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:46.214671+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m978_oscillibacter_score15.py | RESOLVED_TOGO_M978_OSCILLIBACTER_GH | Corrected distilled water from 1 g/L to 988.142 ml/L, removed the variable N2 ingredient in favor of N2 preparation steps, converted empty TOGO M190 and M180 cross-reference stubs to structured JCM 197 and JCM 187 stock solution additions, added pH 6.0, and linked the MediaDive J932 source duplicate. |