aminivibrio_asinoensis_medium (CultureMech:010414)

Overview

External ontology

TOGO:M987 — Aminivibrio Asinoensis Medium

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M987 Original source: JCM - JCM_M940 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=940

Composition

flowchart LR
    CultureMech_010414["aminivibrio_asinoensis_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010414 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_010414 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_010414 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_010414 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_KH2PO4["KH2PO4"]
    CultureMech_010414 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_NH4Cl["NH4Cl"]
    CultureMech_010414 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_Resazurin["Resazurin"]
    CultureMech_010414 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_010414 --> ing7_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing7_MgCl2_6H2O -.-> CHEBI_86345
    ing8_KCl["KCl"]
    CultureMech_010414 --> ing8_KCl
    CHEBI_32588["CHEBI:32588"]
    ing8_KCl -.-> CHEBI_32588
    ing9_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_010414 --> ing9_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing9_Sodium_pyruvate -.-> CHEBI_50144
    ing10_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_010414 --> ing10_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing10_Carbon_dioxide_gas -.-> CHEBI_16526
    ing11_Nitrogen_gas["Nitrogen gas"]
    CultureMech_010414 --> ing11_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing11_Nitrogen_gas -.-> CHEBI_17997
    ing12_Biotin["Biotin"]
    CultureMech_010414 --> ing12_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing12_Biotin -.-> CHEBI_15956
    ing13_p__Aminobenzoic_acid["p--Aminobenzoic acid"]
    CultureMech_010414 --> ing13_p__Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing13_p__Aminobenzoic_acid -.-> CHEBI_30753
    ing14_Thiamine_HCl["Thiamine・HCl"]
    CultureMech_010414 --> ing14_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing14_Thiamine_HCl -.-> CHEBI_49105
    ing15_Vitamin_B12["Vitamin B12"]
    CultureMech_010414 --> ing15_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing15_Vitamin_B12 -.-> CHEBI_176843

Ingredients (16)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	1.0 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 1.0, 1.0]
2	Yeast extract	2 G_PER_L		Role: Nutrient source; Properties: Undefined component
3	NaCl Cl.Na 58.443 g/mol	1 G_PER_L	CHEBI:26710 sodium chloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4	CaCl2・2H2O Ca.2Cl.2H2O 147.014 g/mol	0.15 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
5	KH2PO4 H2O4P.K 136.084 g/mol	0.2 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
6	NH4Cl Cl.H4N 53.492 g/mol	0.25 G_PER_L	CHEBI:31206 ammonium chloride	Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
7	Resazurin C12H7NO4 229.191 g/mol	0.4 G_PER_L	CHEBI:8806 Resazurin	Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component
8	MgCl2・6H2O 2Cl.6H2O.Mg 203.301 g/mol	0.4 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
9	KCl Cl.K 74.551 g/mol	0.5 G_PER_L	CHEBI:32588 potassium chloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
10	Sodium pyruvate C3H3O3.Na 110.044 g/mol	1.1 G_PER_L	CHEBI:50144 sodium pyruvate	Role: Carbon source; Properties: Organic compound, Defined component, Simple component
11	Carbon dioxide gas CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Properties: ガス
12	Nitrogen gas N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Properties: ガス
13	Biotin C10H16N2O3S 244.316 g/mol	20 G_PER_L	CHEBI:15956 biotin	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
14	p--Aminobenzoic acid C7H7NO2 137.138 g/mol	80 G_PER_L	CHEBI:30753 4-aminobenzoic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
15	Thiamine・HCl C12H17N4OS.Cl.HCl 337.276 g/mol	200 G_PER_L	CHEBI:49105 thiamine hydrochloride	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
16	Vitamin B12	50 G_PER_L	CHEBI:176843 vitamin B12	Role: Vitamin; Properties: Organic compound, Defined component, Simple component

Solutions

FeCl2 solution (see Medium [M180])

Dose: 1 G_PER_L

Cross-reference to Medium M180

Trace element solution (see Medium [M180])

Dose: 1 G_PER_L

Cross-reference to Medium M180

Selenite--tungstate solution (see Medium [M431])

Dose: 1 G_PER_L

Cross-reference to Medium M431

8% NaHCO3 solution

Dose: 30 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

Vitamin solution (see below)

Dose: 1 G_PER_L

5% Na2S・9H2O solution

Dose: 7 G_PER_L

Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:56.089336Z	togo-import	Imported from TOGO Medium	Source: JCM, ID: M987
2026-01-28T19:48:37.902902Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:05:03.328704+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 6 solution(s) from ingredients
2026-03-13T03:09:35.233161Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 1 duplicate ingredient(s)
2026-03-13T06:20:13.683830Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:010414
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:46.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:32.660730+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Aminivibrio Asinoensis Medium" to "aminivibrio_asinoensis_medium" for consistent matching
2026-05-16T09:03:22.558242+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:40:46.291780+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.239124+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-02T07:37:54.202355+00:00	repair_merged_duplicates.py	REPAIRED_SUMMED_DUPLICATE_MERGE	Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched.