Overview
- External ontology
- TOGO:M993 — Korthof's Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M993 Original source: JCM - JCM_M946 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=946
Composition
flowchart LR
CultureMech_010421["korthofs_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010421 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Rabbit_serum_heat__inactivated_at_56__8451__for_30_min["Rabbit serum heat--inactivated at 56℃ for 30 min"]
CultureMech_010421 --> ing1_Rabbit_serum_heat__inactivated_at_56__8451__for_30_min
ing2_10_x_Korthof_s_basal_medium__see_below_["10 x Korthof's basal medium (see below)"]
CultureMech_010421 --> ing2_10_x_Korthof_s_basal_medium__see_below_
ing3_NaCl["NaCl"]
CultureMech_010421 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_KH2PO4["KH2PO4"]
CultureMech_010421 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_KCl["KCl"]
CultureMech_010421 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_NaHCO3["NaHCO3"]
CultureMech_010421 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7_Na2HPO4["Na2HPO4"]
CultureMech_010421 --> ing7_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing7_Na2HPO4 -.-> CHEBI_34683
ing8_Polypeptone_peptone__BD_BBL_["Polypeptone peptone (BD-BBL)"]
CultureMech_010421 --> ing8_Polypeptone_peptone__BD_BBL_
ing9_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010421 --> ing9_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing9_CaCl2_2H2O -.-> CHEBI_86158
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
900.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound [Merged 3 duplicates: 400.0, 450.0, 50.0] |
| 2 | Rabbit serum heat--inactivated at 56℃ for 30 min | 50 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 | 10 x Korthof's basal medium (see below) | 50 G_PER_L | ||
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
7 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.2 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.1 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 8 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
4.4 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 9 | Polypeptone peptone (BD-BBL) | 4 G_PER_L | Properties: Undefined component | |
| 10 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
Solutions
CaCl2 solution
Dose: 50 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.105781Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M993 |
| 2026-01-28T19:48:22.454863Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:37.485311+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:07.160805Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:13.751615Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010421 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:13.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.727713+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Korthof's Medium" to "korthofs_medium" for consistent matching |
| 2026-05-16T09:03:22.634946+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:46.375747+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.268581+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |