Overview
- External ontology
- TOGO:M998 — C41 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M998 Original source: JCM - JCM_M951 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=951
Composition
flowchart LR
CultureMech_010426["c41_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010426 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010426 --> ing1_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_2H2O -.-> CHEBI_86158
ing2_KH2PO4["KH2PO4"]
CultureMech_010426 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_K2HPO4["K2HPO4"]
CultureMech_010426 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Resazurin["Resazurin"]
CultureMech_010426 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010426 --> ing5_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_6H2O -.-> CHEBI_86345
ing6_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_010426 --> ing6_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_7H2O -.-> CHEBI_75836
ing7_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010426 --> ing7_Yeast_extract__BD_Difco_
ing8_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_010426 --> ing8_Tryptone__BD_Difco_
ing9_Urea["Urea"]
CultureMech_010426 --> ing9_Urea
CHEBI_16199["CHEBI:16199"]
ing9_Urea -.-> CHEBI_16199
ing10_L_Cysteine_HCl_H2O["L-Cysteine・HCl・H2O"]
CultureMech_010426 --> ing10_L_Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing10_L_Cysteine_HCl_H2O -.-> CHEBI_91248
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Simple component, Defined component |
| 6 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 7 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
1.25 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 8 | Yeast extract (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 9 | Tryptone (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 10 |
Urea
CH4N2O 60.056 g/mol
|
1 G_PER_L |
CHEBI:16199
urea
|
Properties: Defined component, Organic compound, Simple component |
| 11 |
L-Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.6 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Organic compound, Defined component, Simple component |
Solutions
Trace element solution SL--10 (see Medium [M433])
Dose: 1 G_PER_L
Cross-reference to Medium M433
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.114932Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M998 |
| 2026-01-28T19:48:27.620133Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:45.707013+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:13.791170Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010426 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.767471+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "C41 Medium" to "c41_medium" for consistent matching |
| 2026-05-16T09:03:22.667173+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:46.406181+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.281475+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |