Overview
- External ontology
- TOGO:M9 — Lactobacillus Medium II
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M9 Original source: JCM - JCM_M16 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=16
Composition
flowchart LR
CultureMech_010429["lactobacillus_medium_ii"]
ing0_Distilled_water["Distilled water"]
CultureMech_010429 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_KH2PO4["KH2PO4"]
CultureMech_010429 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_K2HPO4["K2HPO4"]
CultureMech_010429 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Sodium_acetate["Sodium acetate"]
CultureMech_010429 --> ing3_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing3_Sodium_acetate -.-> CHEBI_32954
ing4_Tween_80["Tween 80"]
CultureMech_010429 --> ing4_Tween_80
CHEBI_53426["CHEBI:53426"]
ing4_Tween_80 -.-> CHEBI_53426
ing5_Glucose["Glucose"]
CultureMech_010429 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Agar["Agar"]
CultureMech_010429 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010429 --> ing7_Yeast_extract__BD_Difco_
ing8_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_010429 --> ing8_Trypticase_peptone__BD_BBL_
ing9_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_010429 --> ing9_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing9_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing10_Tryptose__BD_Difco_["Tryptose (BD-Difco)"]
CultureMech_010429 --> ing10_Tryptose__BD_Difco_
ing11_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010429 --> ing11_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing11_MgSO4_7H2O -.-> CHEBI_31795
ing12_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_010429 --> ing12_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_7H2O -.-> CHEBI_75836
ing13_MnSO4_xH2O["MnSO4・xH2O"]
CultureMech_010429 --> ing13_MnSO4_xH2O
CHEBI_86360["CHEBI:86360"]
ing13_MnSO4_xH2O -.-> CHEBI_86360
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component [Collapsed 2 identical duplicates: 1.0, 1.0] |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 4 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
1 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Defined component, Simple component, Organic compound |
| 5 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Defined component, Organic compound, Simple component |
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 7 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 8 | Yeast extract (BD-Difco) | 5 G_PER_L | Properties: Undefined component | |
| 9 | Trypticase peptone (BD-BBL) | 10 G_PER_L | Properties: Undefined component | |
| 10 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.2 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Organic compound, Simple component, Defined component |
| 11 | Tryptose (BD-Difco) | 3 G_PER_L | Properties: Undefined component | |
| 12 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
11.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 13 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.68 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 14 |
MnSO4・xH2O
Mn.O4S 151.001 g/mol
|
2.4 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
Solutions
Salt solution R (see below)
Dose: 5 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.925996Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M9 |
| 2026-01-28T19:48:24.706134Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:40.863033+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:10.857912Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:13.808213Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010429 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:17.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.785280+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Lactobacillus Medium II" to "lactobacillus_medium_ii" for consistent matching |
| 2026-05-16T09:03:22.682300+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:46.422660+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:34.513748+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:52.288740+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:54.288027+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |