bekisolidmedium_hydrogenovibrio_crunogenus_quimiolitotrofo

Overview

External ontology

mediadive.medium:P10 (source link unavailable) — BekiSolidMedium (Hydrogenovibrio crunogenus QUIMIOLITOTROFO)

Applications

• Microbial cultivation

pH range

{'min': 6.8, 'max': 7.4}

Notes

MediaDive public P10 reports main-ingredient rates in g/L, but preparation starts with 1 L water, not an explicit final-volume makeup. The trace-stock dose is undisclosed ('trace'); no numeric or VARIABLE dose and no final-medium trace dilution is asserted. Existing physical state and pH are unchanged.

Composition

flowchart LR
    CultureMech_010430["bekisolidmedium_hydrogenovibrio_crunogenus_quimiolitotrofo"]
    ing0_NaCl["NaCl"]
    CultureMech_010430 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_010430 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_NaHCO3["NaHCO3"]
    CultureMech_010430 --> ing2_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing2_NaHCO3 -.-> CHEBI_32139
    ing3_Phosphate_buffer["Phosphate buffer"]
    CultureMech_010430 --> ing3_Phosphate_buffer
    ing4_Na2S2O4["Na2S2O4"]
    CultureMech_010430 --> ing4_Na2S2O4
    CHEBI_66870["CHEBI:66870"]
    ing4_Na2S2O4 -.-> CHEBI_66870
    ing5_KNO3["KNO3"]
    CultureMech_010430 --> ing5_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing5_KNO3 -.-> CHEBI_63043
    ing6_Agar["Agar"]
    CultureMech_010430 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Distilled_water["Distilled water"]
    CultureMech_010430 --> ing7_Distilled_water

Ingredients (8)

Preparation

1. Prepare the base in 1 L distilled water with NaCl, CaCl2 x 2 H2O, NaHCO3, and phosphate buffer.
2. Add Na2S2O4 and prepare the Elemento traza solution from ZnSO4 x 7 H2O, FeSO4 x 7 H2O, MnCl2 x 4 H2O, CuSO4 x 5 H2O, CuCl2, Fe(III)NH4-EDTA, and (NH4)2MoO4.
3. Add KNO3 and agar to complete the solid medium.

Solutions

Target organisms

No target organisms are recorded.

Provenance