Overview
- External ontology
- mediadive.medium:P1 (source link unavailable) — HaHa agar
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- MediaDive public Medium P1 provides a complete HaHa agar test recipe with a 700 ml main solution, washed Bacto agar, ASW 2x, Trace element solution SL-8, Selenite-tungstate solution II, and sterile stock additions; water fill-up rows were omitted.
Composition
flowchart LR
CultureMech_010431["haha_agar"]
ing0_HEPES["HEPES"]
CultureMech_010431 --> ing0_HEPES
CHEBI_42334["CHEBI:42334"]
ing0_HEPES -.-> CHEBI_42334
ing1_Bacto_agar["Bacto agar"]
CultureMech_010431 --> ing1_Bacto_agar
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
HEPES
C8H18N2O4S 238.309 g/mol
|
14.1714 G_PER_L |
CHEBI:42334
2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
|
P1 adds 9.92 g HEPES to a 700 ml main solution. |
| 2 | Bacto agar | 18 G_PER_L | P1 adds 18 g/L washed Bacto agar. |
Preparation
- Mix Artificial sea water (ASW) 2x, HEPES, and a magnetic stir bar, then fill to 650 ml with MilliQ water.
- Wash the Bacto agar with ultrapure water, allow the agar to settle, remove the overlaying water, and repeat the wash twice.
- Directly before autoclaving, mix agar and liquid, autoclave, cool to 60 C, and hold at 55 C.
- Add sterile Trace element solution SL-8, Selenite-tungstate solution II, phosphate, ammonium, glucose, cellobiose, yeast extract, Casamino acids, Tryptone peptone, magnesium chloride, and magnesium sulfate stocks.
- Adjust to pH 7.5 with sterile 1 M HCl or 1 M NaOH, then add sterile MilliQ water to a final volume of 700 ml while avoiding bubbles.
Solutions
Artificial sea water (ASW) 2x
Dose: 500 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaCl | 52.74 G_PER_L | |
| NaHCO3 | 1000 G_PER_L | |
| CaCl2 x 2 H2O | 2.94 G_PER_L | |
| KCl | 1.44 G_PER_L | |
| KBr | 0.20 G_PER_L | |
| H3BO3 | 0.040 G_PER_L | |
| SrCl2 | 0.040 G_PER_L | |
| NaF | 0.006 G_PER_L |
P1 adds 350 ml Artificial sea water (ASW) 2x to a 700 ml main solution.
Trace element solution SL-8
Dose: 2 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2-EDTA | 5.2 G_PER_L | |
| FeSO4 x 7 H2O | 2 G_PER_L | |
| CoCl2 x 6 H2O | 0.190 G_PER_L | |
| MnCl2 x 2 H2O | 0.100 G_PER_L | |
| ZnSO4 x 7 H2O | 0.150 G_PER_L | |
| NiCl2 x 6 H2O | 0.024 G_PER_L | |
| Na2MoO4 x 2 H2O | 0.036 G_PER_L | |
| H3BO3 | 0.062 G_PER_L | |
| CuCl2 x 2 H2O | 0.017 G_PER_L |
P1 adds 1.4 ml Trace element solution SL-8 to a 700 ml main solution.
Selenite-tungstate solution II
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaOH | 0.2 G_PER_L | |
| Na2SeO3 x 5 H2O | 0.018 G_PER_L | |
| Na2WO4 x 2 H2O | 0.018 G_PER_L |
P1 adds 0.7 ml Selenite-tungstate solution II to a 700 ml main solution.
KH2PO4 stock
Dose: 10 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| KH2PO4 | 50 G_PER_L |
P1 adds 7 ml of a 50 g/L autoclaved KH2PO4 stock to a 700 ml main solution.
NH4Cl stock
Dose: 5 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NH4Cl | 50 G_PER_L |
P1 adds 3.5 ml of a 50 g/L autoclaved NH4Cl stock to a 700 ml main solution.
Glucose stock
Dose: 5 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Glucose | 100 G_PER_L |
P1 adds 3.5 ml of a sterile-filtered 100 g/L glucose stock to a 700 ml main solution.
Cellobiose stock
Dose: 5 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Cellobiose | 100 G_PER_L |
P1 adds 3.5 ml of a sterile-filtered 100 g/L cellobiose stock to a 700 ml main solution.
Yeast extract stock
Dose: 5 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Yeast extract | 100 G_PER_L |
P1 adds 3.5 ml of a sterile-filtered 100 g/L yeast extract stock to a 700 ml main solution.
Casamino acids stock
Dose: 5 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Casamino acids | 100 G_PER_L |
P1 adds 3.5 ml of a sterile-filtered 100 g/L Casamino acids stock to a 700 ml main solution.
Tryptone peptone stock
Dose: 5 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Tryptone peptone | 100 G_PER_L |
P1 adds 3.5 ml of a sterile-filtered 100 g/L Tryptone peptone stock to a 700 ml main solution.
MgCl2 x 6 H2O stock
Dose: 11.2857 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MgCl2 x 6 H2O | 500 G_PER_L |
P1 adds 7.9 ml of a 500 g/L autoclaved MgCl2 x 6 H2O stock to a 700 ml main solution.
MgSO4 x 7 H2O stock
Dose: 13.5714 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MgSO4 x 7 H2O | 500 G_PER_L |
P1 adds 9.5 ml of a 500 g/L autoclaved MgSO4 x 7 H2O stock to a 700 ml main solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.837238Z | mediadive-import | Imported from MediaDive | Source: public, ID: P1 |
| 2026-02-03T11:27:23.227710 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:20:13.814956Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010431 |
| 2026-03-17T19:46:32.792852+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HaHa agar" to "haha_agar" for consistent matching |
| 2026-08-06T07:56:36.100057+00:00 | curate_placeholder_composition.py | REMOVED_PLACEHOLDER_INGREDIENT | Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175). |
| 2026-09-08T00:00:00-07:00 | repair_mediadive_public_score30.py | RESOLVED_MEDIADIVE_PUBLIC_SCORE30 | MediaDive public Medium P1 provides a complete HaHa agar test recipe with a 700 ml main solution, washed Bacto agar, ASW 2x, Trace element solution SL-8, Selenite-tungstate solution II, and sterile stock additions; water fill-up rows were omitted. |