king_b (CultureMech:010433)

Overview

External ontology

mediadive.medium:P3 (source link unavailable) — King B

Applications

Microbial cultivation

pH

7.2

Notes

MediaDive public Medium P3 provides King B as a 1 L solid agar recipe at pH 7.2.

Composition

flowchart LR
    CultureMech_010433["king_b"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_010433 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_010433 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_Proteose_peptone_no__3["Proteose peptone no. 3"]
    CultureMech_010433 --> ing2_Proteose_peptone_no__3
    ing3_Glycerol["Glycerol"]
    CultureMech_010433 --> ing3_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing3_Glycerol -.-> CHEBI_17754
    ing4_Agar["Agar"]
    CultureMech_010433 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509

Ingredients (5)

#	Ingredient	Concentration	Ontology
1	K2HPO4 HO4P.2K 174.174 g/mol	1.5 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate
2	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	1.5 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
3	Proteose peptone no. 3	20 G_PER_L	MICRO:0000180 (source link unavailable) proteose peptone
4	Glycerol C3H8O3 92.094 g/mol	10 ML_PER_L	CHEBI:17754 glycerol
5	Agar	15 G_PER_L	CHEBI:2509 agar

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:19.840359Z	mediadive-import	Imported from MediaDive	Source: public, ID: P3
2026-02-03T11:27:23.230560	quality-tagger-v1.0	Added data quality flag	Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:20:13.819534Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:010433
2026-03-17T19:46:32.797354+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "King B" to "king_b" for consistent matching
2026-08-06T07:56:36.106863+00:00	curate_placeholder_composition.py	REMOVED_PLACEHOLDER_INGREDIENT	Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175).
2026-09-08T00:00:00-07:00	repair_mediadive_public_score30.py	RESOLVED_MEDIADIVE_PUBLIC_SCORE30	MediaDive public Medium P3 provides King B as a 1 L solid agar recipe at pH 7.2.
2026-10-07T17:04:47.759246+00:00	codex	ADDED_PUBLIC_DIRECT_CONCENTRATION_EVIDENCE	Exact source formulation checked; all quantities, ingredient forms, ontology annotations, pH, state, existing references and prior history preserved. No new growth association or variant.