BG11

Overview

pH

7.1

Notes

Source: FEBA media definitions

Composition

flowchart LR
    CultureMech_015450["BG11"]
    ing0_Sodium_nitrate["Sodium nitrate"]
    CultureMech_015450 --> ing0_Sodium_nitrate
    CHEBI_63005["CHEBI:63005"]
    ing0_Sodium_nitrate -.-> CHEBI_63005
    ing1_Potassium_phosphate_dibasic["Potassium phosphate dibasic"]
    CultureMech_015450 --> ing1_Potassium_phosphate_dibasic
    CHEBI_131527["CHEBI:131527"]
    ing1_Potassium_phosphate_dibasic -.-> CHEBI_131527
    ing2_Magnesium_Sulfate_Heptahydrate["Magnesium Sulfate Heptahydrate"]
    CultureMech_015450 --> ing2_Magnesium_Sulfate_Heptahydrate
    CHEBI_31795["CHEBI:31795"]
    ing2_Magnesium_Sulfate_Heptahydrate -.-> CHEBI_31795
    ing3_Calcium_chloride_dihydrate["Calcium chloride dihydrate"]
    CultureMech_015450 --> ing3_Calcium_chloride_dihydrate
    CHEBI_86158["CHEBI:86158"]
    ing3_Calcium_chloride_dihydrate -.-> CHEBI_86158
    ing4_Citric_Acid["Citric Acid"]
    CultureMech_015450 --> ing4_Citric_Acid
    CHEBI_53258["CHEBI:53258"]
    ing4_Citric_Acid -.-> CHEBI_53258
    ing5_Ferric_ammonium_citrate["Ferric ammonium citrate"]
    CultureMech_015450 --> ing5_Ferric_ammonium_citrate
    CHEBI_31604["CHEBI:31604"]
    ing5_Ferric_ammonium_citrate -.-> CHEBI_31604
    ing6_EDTA__disodium_salt_["EDTA (disodium salt)"]
    CultureMech_015450 --> ing6_EDTA__disodium_salt_
    CHEBI_64734["CHEBI:64734"]
    ing6_EDTA__disodium_salt_ -.-> CHEBI_64734
    ing7_Sodium_carbonate["Sodium carbonate"]
    CultureMech_015450 --> ing7_Sodium_carbonate
    CHEBI_29377["CHEBI:29377"]
    ing7_Sodium_carbonate -.-> CHEBI_29377
    ing8_Boric_Acid["Boric Acid"]
    CultureMech_015450 --> ing8_Boric_Acid
    CHEBI_33118["CHEBI:33118"]
    ing8_Boric_Acid -.-> CHEBI_33118
    ing9_Manganese__II__chloride_tetrahydrate["Manganese (II) chloride tetrahydrate"]
    CultureMech_015450 --> ing9_Manganese__II__chloride_tetrahydrate
    CHEBI_86368["CHEBI:86368"]
    ing9_Manganese__II__chloride_tetrahydrate -.-> CHEBI_86368
    ing10_Zinc_sulfate_heptahydrate["Zinc sulfate heptahydrate"]
    CultureMech_015450 --> ing10_Zinc_sulfate_heptahydrate
    CHEBI_32312["CHEBI:32312"]
    ing10_Zinc_sulfate_heptahydrate -.-> CHEBI_32312
    ing11_Sodium_Molybdate_Dihydrate["Sodium Molybdate Dihydrate"]
    CultureMech_015450 --> ing11_Sodium_Molybdate_Dihydrate
    CHEBI_75213["CHEBI:75213"]
    ing11_Sodium_Molybdate_Dihydrate -.-> CHEBI_75213
    ing12_Copper__II__sulfate_pentahydrate["Copper (II) sulfate pentahydrate"]
    CultureMech_015450 --> ing12_Copper__II__sulfate_pentahydrate
    CHEBI_31440["CHEBI:31440"]
    ing12_Copper__II__sulfate_pentahydrate -.-> CHEBI_31440
    ing13_Cobalt_II__nitrate_hexahydrate["Cobalt(II) nitrate hexahydrate"]
    CultureMech_015450 --> ing13_Cobalt_II__nitrate_hexahydrate
    CHEBI_86214["CHEBI:86214"]
    ing13_Cobalt_II__nitrate_hexahydrate -.-> CHEBI_86214

Ingredients (14)

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

• Preferred term

  Nostoc linckia

  Term

  Id

  NCBITaxon:92942

  Label

  Nostoc linckia

  Growth metrics

  • Measurement conditions

    BG11 nitrate-replete medium after 240 h of culture; compared with nitrate-free BG110.

    Evidence

    • Reference

      PMID:36550226

      Supports

      SUPPORT

      Snippet

      Cultures grown in BG11 accumulated more cell biomass reaching a dry weight of 1.65 ± 0.06 g L-1, compared to 0.92 ± 0.01 g L-1 in BG110 after 240 h of culture.

      Explanation

      Literature evidence reports biomass accumulation of Nostoc linckia in BG11.

    Is max attainment

    False

• Preferred term

  Synechococcus sp. UCP002

  Strain

  UCP002

  Term

  Id

  NCBITaxon:2885151

  Label

  Synechococcus sp. UCP002

  Growth metrics

  • Growth rate per hour

    0.086

    Doubling time minutes

    484.8

    Measurement conditions

    BG-11 medium culture of an unaxenic Synechococcus sp. UCP002 isolate from the Peruvian Amazon Basin.

    Evidence

    • Reference

      PMID:36437912

      Supports

      SUPPORT

      Snippet

      Synechococcus sp. UCP002 had a specific growth rate of 0.086 ± 0.008 μ and a doubling time of 8.08 ± 0.78 h.

      Explanation

      Literature evidence reports growth rate and doubling time for Synechococcus sp. UCP002 cultured in BG-11 medium.

    Is max attainment

    False

  Evidence

  • Reference

    PMID:36437912

    Supports

    SUPPORT

    Snippet

    Growth parameters, genome features, and the biochemical profile of the cyanobacterium were determined using standardized methods.

    Explanation

    Literature evidence reports a complete genome for UCP002, but no NCBI GCA/GCF/SAMN accession was resolved from NCBI assembly or nuccore search.

• Preferred term

  Tetradesmus obliquus

  Strain

  ABC-009

  Term

  Id

  NCBITaxon:3088

  Label

  Tetradesmus obliquus

  Growth metrics

  • Measurement conditions

    Photoautotrophic BG-11 medium cultivation with air or 2% CO2 for the strain reported in the source as Scenedesmus obliquus ABC-009.

    Growth mode

    BATCH

    Evidence

    • Reference

      PMID:34584038

      Supports

      SUPPORT

      Snippet

      Scenedesmus obliquus ABC-009 is a microalgal strain that accumulates large amounts of lutein, particularly when subjected to growth-limiting conditions.

      Explanation

      Literature evidence identifies the strain studied under BG-11 photoautotrophic cultivation; NCBI nuccore record MG971386.1 links strain ABC-009 to the accepted taxonomy Tetradesmus obliquus, but it is a partial marker sequence rather than a genome assembly.

    • Reference

      PMID:34584038

      Supports

      SUPPORT

      Snippet

      While the cell concentrations of the cultures grown under BG-11 and CO2 were largely similar to those grown in YM medium

      Explanation

      Literature evidence reports cell concentrations for the BG-11 plus CO2 condition relative to YM medium.

    Is max attainment

    False

Recipe variants

• Name

  nitrate_replete_bg11_nostoc_linckia

  Description

  BG11 nitrate-replete condition used for Nostoc linckia growth and biomass productivity comparison with nitrate-free BG110.

  Modifications

  • Use BG11 medium with nitrate present.
  • Compare with BG110 medium lacking nitrate.

  Purpose

  Capture nitrate-replete BG11 growth evidence for Nostoc linckia.

  Evidence

  • Reference

    PMID:36550226

    Supports

    SUPPORT

    Snippet

    Cultures grown in BG11 accumulated more cell biomass reaching a dry weight of 1.65 ± 0.06 g L-1, compared to 0.92 ± 0.01 g L-1 in BG110 after 240 h of culture.

    Explanation

    Literature evidence reports higher biomass and productivity of Nostoc linckia in BG11 than nitrate-free BG110.

• Name

  bg11_synechococcus_ucp002

  Description

  BG-11 cultivation condition for unaxenic Synechococcus sp. UCP002 isolated from the Peruvian Amazon Basin.

  Modifications

  • Culture Synechococcus sp. UCP002 in BG-11 medium.
  • Use standardized growth-parameter and biochemical-profile measurements.

  Purpose

  Capture strain-specific BG-11 growth and genome-context evidence for Synechococcus sp. UCP002.

  Evidence

  • Reference

    PMID:36437912

    Supports

    SUPPORT

    Snippet

    The cyanobacterium was isolated from the Peruvian Amazon Basin region and cultured in BG-11 medium.

    Explanation

    Literature evidence directly states that Synechococcus sp. UCP002 was cultured in BG-11 medium.

• Name

  bg11_scenedesmus_obliquus_abc009_photoautotrophic

  Description

  BG-11 photoautotrophic cultivation condition for the lutein-producing algal strain reported as Scenedesmus obliquus ABC-009.

  Modifications

  • Use BG-11 medium under photoautotrophic cultivation.
  • Compare air and 2% CO2 gas conditions.

  Purpose

  Capture strain-level BG-11 growth evidence for Scenedesmus obliquus ABC-009, currently mapped by NCBI to Tetradesmus obliquus.

  Evidence

  • Reference

    PMID:34584038

    Supports

    SUPPORT

    Snippet

    photoautotrophic mode using BG-11 medium with air or 2% CO2 and heterotrophic mode using YM medium.

    Explanation

    Literature evidence directly states that strain ABC-009 was evaluated under photoautotrophic BG-11 cultivation conditions.

Provenance