Overview
- Notes
- Source: FEBA media definitions
Composition
flowchart LR
CultureMech_015457["DinoMM L lactateCarbon HighNutrient"]
ing0_Sea_salts["Sea salts"]
CultureMech_015457 --> ing0_Sea_salts
ing1_Ammonium_Sulfate["Ammonium Sulfate"]
CultureMech_015457 --> ing1_Ammonium_Sulfate
CHEBI_62946["CHEBI:62946"]
ing1_Ammonium_Sulfate -.-> CHEBI_62946
ing2_Potassium_phosphate_monobasic["Potassium phosphate monobasic"]
CultureMech_015457 --> ing2_Potassium_phosphate_monobasic
CHEBI_63036["CHEBI:63036"]
ing2_Potassium_phosphate_monobasic -.-> CHEBI_63036
ing3_Magnesium_Sulfate_Heptahydrate["Magnesium Sulfate Heptahydrate"]
CultureMech_015457 --> ing3_Magnesium_Sulfate_Heptahydrate
CHEBI_31795["CHEBI:31795"]
ing3_Magnesium_Sulfate_Heptahydrate -.-> CHEBI_31795
ing4_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_015457 --> ing4_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing4_Nitrilotriacetic_acid -.-> CHEBI_44557
ing5_Sodium_Chloride["Sodium Chloride"]
CultureMech_015457 --> ing5_Sodium_Chloride
CHEBI_26710["CHEBI:26710"]
ing5_Sodium_Chloride -.-> CHEBI_26710
ing6_Manganese__II__sulfate_monohydrate["Manganese (II) sulfate monohydrate"]
CultureMech_015457 --> ing6_Manganese__II__sulfate_monohydrate
CHEBI_86364["CHEBI:86364"]
ing6_Manganese__II__sulfate_monohydrate -.-> CHEBI_86364
ing7_Cobalt_chloride_hexahydrate["Cobalt chloride hexahydrate"]
CultureMech_015457 --> ing7_Cobalt_chloride_hexahydrate
CHEBI_53503["CHEBI:53503"]
ing7_Cobalt_chloride_hexahydrate -.-> CHEBI_53503
ing8_Zinc_sulfate_heptahydrate["Zinc sulfate heptahydrate"]
CultureMech_015457 --> ing8_Zinc_sulfate_heptahydrate
CHEBI_32312["CHEBI:32312"]
ing8_Zinc_sulfate_heptahydrate -.-> CHEBI_32312
ing9_Calcium_Chloride_Dihydrate["Calcium Chloride Dihydrate"]
CultureMech_015457 --> ing9_Calcium_Chloride_Dihydrate
CHEBI_86158["CHEBI:86158"]
ing9_Calcium_Chloride_Dihydrate -.-> CHEBI_86158
ing10_Iron__II__sulfate_heptahydrate["Iron (II) sulfate heptahydrate"]
CultureMech_015457 --> ing10_Iron__II__sulfate_heptahydrate
CHEBI_75836["CHEBI:75836"]
ing10_Iron__II__sulfate_heptahydrate -.-> CHEBI_75836
ing11_Nickel__II__chloride_hexahydrate["Nickel (II) chloride hexahydrate"]
CultureMech_015457 --> ing11_Nickel__II__chloride_hexahydrate
CHEBI_53542["CHEBI:53542"]
ing11_Nickel__II__chloride_hexahydrate -.-> CHEBI_53542
ing12_Aluminum_potassium_sulfate_dodecahydrate["Aluminum potassium sulfate dodecahydrate"]
CultureMech_015457 --> ing12_Aluminum_potassium_sulfate_dodecahydrate
CHEBI_86465["CHEBI:86465"]
ing12_Aluminum_potassium_sulfate_dodecahydrate -.-> CHEBI_86465
ing13_Copper__II__sulfate_pentahydrate["Copper (II) sulfate pentahydrate"]
CultureMech_015457 --> ing13_Copper__II__sulfate_pentahydrate
CHEBI_31440["CHEBI:31440"]
ing13_Copper__II__sulfate_pentahydrate -.-> CHEBI_31440
ing14_Boric_Acid["Boric Acid"]
CultureMech_015457 --> ing14_Boric_Acid
CHEBI_33118["CHEBI:33118"]
ing14_Boric_Acid -.-> CHEBI_33118
ing15_Sodium_Molybdate_Dihydrate["Sodium Molybdate Dihydrate"]
CultureMech_015457 --> ing15_Sodium_Molybdate_Dihydrate
CHEBI_75213["CHEBI:75213"]
ing15_Sodium_Molybdate_Dihydrate -.-> CHEBI_75213
ing16_Sodium_selenite_pentahydrate["Sodium selenite pentahydrate"]
CultureMech_015457 --> ing16_Sodium_selenite_pentahydrate
CHEBI_131361["CHEBI:131361"]
ing16_Sodium_selenite_pentahydrate -.-> CHEBI_131361
ing17_pyridoxine_HCl["pyridoxine HCl"]
CultureMech_015457 --> ing17_pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing17_pyridoxine_HCl -.-> CHEBI_30961
ing18_4_Aminobenzoic_acid["4-Aminobenzoic acid"]
CultureMech_015457 --> ing18_4_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing18_4_Aminobenzoic_acid -.-> CHEBI_30753
ing19_Lipoic_acid["Lipoic acid"]
CultureMech_015457 --> ing19_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing19_Lipoic_acid -.-> CHEBI_16494
ing20_Nicotinic_Acid["Nicotinic Acid"]
CultureMech_015457 --> ing20_Nicotinic_Acid
CHEBI_15940["CHEBI:15940"]
ing20_Nicotinic_Acid -.-> CHEBI_15940
ing21_Riboflavin["Riboflavin"]
CultureMech_015457 --> ing21_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing21_Riboflavin -.-> CHEBI_17015
ing22_thiamine_HCl["thiamine HCl"]
CultureMech_015457 --> ing22_thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing22_thiamine_HCl -.-> CHEBI_49105
ing23_calcium_pantothenate["calcium pantothenate"]
CultureMech_015457 --> ing23_calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing23_calcium_pantothenate -.-> CHEBI_31345
ing24_biotin["biotin"]
CultureMech_015457 --> ing24_biotin
CHEBI_15956["CHEBI:15956"]
ing24_biotin -.-> CHEBI_15956
ing25_Folic_Acid["Folic Acid"]
CultureMech_015457 --> ing25_Folic_Acid
CHEBI_27470["CHEBI:27470"]
ing25_Folic_Acid -.-> CHEBI_27470
ing26_Cyanocobalamin["Cyanocobalamin"]
CultureMech_015457 --> ing26_Cyanocobalamin
CHEBI_17439["CHEBI:17439"]
ing26_Cyanocobalamin -.-> CHEBI_17439
ing27_Sodium_L_Lactate["Sodium L-Lactate"]
CultureMech_015457 --> ing27_Sodium_L_Lactate
CHEBI_232798["CHEBI:232798"]
ing27_Sodium_L_Lactate -.-> CHEBI_232798
Ingredients (28)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sea salts | 20 G_PER_L | ||
| 2 |
Ammonium Sulfate
2H4N.O4S 132.141 g/mol
|
0.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 7783-20-2; MW: 132.14 |
| 3 |
Potassium phosphate monobasic
H2O4P.K 136.084 g/mol
|
0.1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 7778-77-0; MW: 136.09 |
| 4 |
Magnesium Sulfate Heptahydrate
7H2O.Mg.O4S 246.473 g/mol
|
0.03 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
CAS: 10034-99-8; MW: 246.47; From Wolfe's mineral mix |
| 5 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
0.015 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 139-13-9; MW: 191.14; From Wolfe's mineral mix |
| 6 |
Sodium Chloride
Cl.Na 58.443 g/mol
|
0.01 G_PER_L |
CHEBI:26710
sodium chloride
|
CAS: 7647-14-5; MW: 58.44; From Wolfe's mineral mix |
| 7 |
Manganese (II) sulfate monohydrate
H2O.Mn.O4S 169.016 g/mol
|
0.005 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
CAS: 10034-96-5; MW: 169.02; From Wolfe's mineral mix |
| 8 |
Cobalt chloride hexahydrate
2Cl.Co.6H2O 237.929 g/mol
|
0.001 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
CAS: 7791-13-1; MW: 237.93; From Wolfe's mineral mix; Ontology enriched via CAS-RN lookup |
| 9 |
Zinc sulfate heptahydrate
7H2O.O4S.Zn 287.558 g/mol
|
0.001 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
CAS: 7446-20-0; MW: 287.56; From Wolfe's mineral mix; Ontology enriched via CAS-RN lookup |
| 10 |
Calcium Chloride Dihydrate
Ca.2Cl.2H2O 147.014 g/mol
|
0.001 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Mapping: micromediaparam_legacy (confidence: 0.90); From Wolfe's mineral mix |
| 11 |
Iron (II) sulfate heptahydrate
Fe.7H2O.O4S 278.013 g/mol
|
0.001 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
CAS: 7782-63-0; MW: 278.01; From Wolfe's mineral mix; Ontology enriched via CAS-RN lookup |
| 12 |
Nickel (II) chloride hexahydrate
2Cl.6H2O.Ni 237.689 g/mol
|
0.00025 G_PER_L |
CHEBI:53542
nickel chloride hexahydrate
|
CAS: 7791-20-0; MW: 237.69; From Wolfe's mineral mix; Ontology enriched via CAS-RN lookup |
| 13 |
Aluminum potassium sulfate dodecahydrate
Al.12H2O.K.2O4S 474.386 g/mol
|
0.0002 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
CAS: 7784-24-9; MW: 474.39; From Wolfe's mineral mix; Ontology enriched via CAS-RN lookup |
| 14 |
Copper (II) sulfate pentahydrate
Cu.5H2O.O4S 249.684 g/mol
|
0.0001 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
CAS: 7758-99-8; MW: 249.69; From Wolfe's mineral mix; Ontology enriched via CAS-RN lookup |
| 15 |
Boric Acid
H3BO3 61.833 g/mol
|
0.0001 G_PER_L |
CHEBI:33118
boric acid
|
CAS: 10043-35-3; MW: 61.83; From Wolfe's mineral mix |
| 16 |
Sodium Molybdate Dihydrate
2H2O.MoO4.2Na 241.946 g/mol
|
0.0001 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
CAS: 10102-40-6; MW: 241.95; From Wolfe's mineral mix; Ontology enriched via CAS-RN lookup |
| 17 |
Sodium selenite pentahydrate
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 MG_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
CAS: 26970-82-1; MW: 263.01; From Wolfe's mineral mix; Ontology enriched via CAS-RN lookup |
| 18 |
pyridoxine HCl
C8H11NO3.HCl 205.641 g/mol
|
0.1 MG_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Mapping: micromediaparam_legacy (confidence: 0.90); From Wolfe's vitamin mix |
| 19 |
4-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 MG_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
CAS: 150-13-0; MW: 137.14; From Wolfe's vitamin mix; Ontology enriched via CAS-RN lookup |
| 20 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 MG_PER_L |
CHEBI:16494
lipoic acid
|
Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 1077-28-7; MW: 206.33; From Wolfe's vitamin mix |
| 21 |
Nicotinic Acid
C6H5NO2 123.111 g/mol
|
0.05 MG_PER_L |
CHEBI:15940
nicotinic acid
|
Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 59-67-6; MW: 123.11; From Wolfe's vitamin mix |
| 22 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 MG_PER_L |
CHEBI:17015
riboflavin
|
Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 83-88-5; MW: 376.36; From Wolfe's vitamin mix |
| 23 |
thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 MG_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Mapping: micromediaparam_legacy (confidence: 0.90); From Wolfe's vitamin mix |
| 24 |
calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 MG_PER_L |
CHEBI:31345
Calcium pantothenate
|
Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 137-08-6; MW: 238.27; From Wolfe's vitamin mix |
| 25 |
biotin
C10H16N2O3S 244.316 g/mol
|
0.02 MG_PER_L |
CHEBI:15956
biotin
|
Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 58-85-5; MW: 244.31; From Wolfe's vitamin mix |
| 26 |
Folic Acid
C19H19N7O6 441.404 g/mol
|
0.02 MG_PER_L |
CHEBI:27470
folic acid
|
Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 59-30-3; MW: 441.4; From Wolfe's vitamin mix |
| 27 |
Cyanocobalamin
C63H88CoN14O14P 1355.388 g/mol
|
0.001 MG_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 68-19-9; MW: 1355.37; From Wolfe's vitamin mix |
| 28 |
Sodium L-Lactate
C3H5O3.Na 112.06 g/mol
|
20 MILLIMOLAR |
CHEBI:232798
sodium L-lactate
|
Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 867-56-1; MW: 112.06 |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-03-18T04:31:12.619041+00:00 | import_from_culturebotht.py | IMPORTED | Imported from CultureBotHT repository: DinoMM_L_lactateCarbon_HighNutrient.yaml |
| 2026-03-18T04:33:58.400364Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:015457 |
| 2026-05-16T09:03:30.809057+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | category->lowercase=1 |
| 2026-06-08T23:19:37.824177+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 1 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:56.389601+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |