DinoMM noCarbon HighNutrient ATCC (CultureMech:015460)

Overview

Notes: Source: FEBA media definitions

Composition

flowchart LR
    CultureMech_015460["DinoMM noCarbon HighNutrient ATCC"]
    ing0_Sea_salts["Sea salts"]
    CultureMech_015460 --> ing0_Sea_salts
    ing1_Ammonium_Sulfate["Ammonium Sulfate"]
    CultureMech_015460 --> ing1_Ammonium_Sulfate
    CHEBI_62946["CHEBI:62946"]
    ing1_Ammonium_Sulfate -.-> CHEBI_62946
    ing2_Potassium_phosphate_monobasic["Potassium phosphate monobasic"]
    CultureMech_015460 --> ing2_Potassium_phosphate_monobasic
    CHEBI_63036["CHEBI:63036"]
    ing2_Potassium_phosphate_monobasic -.-> CHEBI_63036
    ing3_EDTA["EDTA"]
    CultureMech_015460 --> ing3_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing3_EDTA -.-> CHEBI_4735
    ing4_Magnesium_Sulfate_Heptahydrate["Magnesium Sulfate Heptahydrate"]
    CultureMech_015460 --> ing4_Magnesium_Sulfate_Heptahydrate
    CHEBI_31795["CHEBI:31795"]
    ing4_Magnesium_Sulfate_Heptahydrate -.-> CHEBI_31795
    ing5_Manganese__II__sulfate_monohydrate["Manganese (II) sulfate monohydrate"]
    CultureMech_015460 --> ing5_Manganese__II__sulfate_monohydrate
    CHEBI_86364["CHEBI:86364"]
    ing5_Manganese__II__sulfate_monohydrate -.-> CHEBI_86364
    ing6_Sodium_Chloride["Sodium Chloride"]
    CultureMech_015460 --> ing6_Sodium_Chloride
    CHEBI_26710["CHEBI:26710"]
    ing6_Sodium_Chloride -.-> CHEBI_26710
    ing7_Iron__II__sulfate_heptahydrate["Iron (II) sulfate heptahydrate"]
    CultureMech_015460 --> ing7_Iron__II__sulfate_heptahydrate
    CHEBI_75836["CHEBI:75836"]
    ing7_Iron__II__sulfate_heptahydrate -.-> CHEBI_75836
    ing8_Cobalt_II__nitrate_hexahydrate["Cobalt(II) nitrate hexahydrate"]
    CultureMech_015460 --> ing8_Cobalt_II__nitrate_hexahydrate
    CHEBI_86214["CHEBI:86214"]
    ing8_Cobalt_II__nitrate_hexahydrate -.-> CHEBI_86214
    ing9_Calcium_Chloride_Dihydrate["Calcium Chloride Dihydrate"]
    CultureMech_015460 --> ing9_Calcium_Chloride_Dihydrate
    CHEBI_86158["CHEBI:86158"]
    ing9_Calcium_Chloride_Dihydrate -.-> CHEBI_86158
    ing10_Zinc_sulfate_heptahydrate["Zinc sulfate heptahydrate"]
    CultureMech_015460 --> ing10_Zinc_sulfate_heptahydrate
    CHEBI_32312["CHEBI:32312"]
    ing10_Zinc_sulfate_heptahydrate -.-> CHEBI_32312
    ing11_Copper__II__sulfate_pentahydrate["Copper (II) sulfate pentahydrate"]
    CultureMech_015460 --> ing11_Copper__II__sulfate_pentahydrate
    CHEBI_31440["CHEBI:31440"]
    ing11_Copper__II__sulfate_pentahydrate -.-> CHEBI_31440
    ing12_Aluminum_potassium_sulfate_dodecahydrate["Aluminum potassium sulfate dodecahydrate"]
    CultureMech_015460 --> ing12_Aluminum_potassium_sulfate_dodecahydrate
    CHEBI_86465["CHEBI:86465"]
    ing12_Aluminum_potassium_sulfate_dodecahydrate -.-> CHEBI_86465
    ing13_Boric_Acid["Boric Acid"]
    CultureMech_015460 --> ing13_Boric_Acid
    CHEBI_33118["CHEBI:33118"]
    ing13_Boric_Acid -.-> CHEBI_33118
    ing14_Sodium_Molybdate_Dihydrate["Sodium Molybdate Dihydrate"]
    CultureMech_015460 --> ing14_Sodium_Molybdate_Dihydrate
    CHEBI_75213["CHEBI:75213"]
    ing14_Sodium_Molybdate_Dihydrate -.-> CHEBI_75213
    ing15_Sodium_selenite_pentahydrate["Sodium selenite pentahydrate"]
    CultureMech_015460 --> ing15_Sodium_selenite_pentahydrate
    CHEBI_131361["CHEBI:131361"]
    ing15_Sodium_selenite_pentahydrate -.-> CHEBI_131361
    ing16_Sodium_tungstate_dihydrate["Sodium tungstate dihydrate"]
    CultureMech_015460 --> ing16_Sodium_tungstate_dihydrate
    CHEBI_63939["CHEBI:63939"]
    ing16_Sodium_tungstate_dihydrate -.-> CHEBI_63939
    ing17_Nickel__II__chloride_hexahydrate["Nickel (II) chloride hexahydrate"]
    CultureMech_015460 --> ing17_Nickel__II__chloride_hexahydrate
    CHEBI_53542["CHEBI:53542"]
    ing17_Nickel__II__chloride_hexahydrate -.-> CHEBI_53542
    ing18_Folic_Acid["Folic Acid"]
    CultureMech_015460 --> ing18_Folic_Acid
    CHEBI_27470["CHEBI:27470"]
    ing18_Folic_Acid -.-> CHEBI_27470
    ing19_Pyridoxine_HCl["Pyridoxine HCl"]
    CultureMech_015460 --> ing19_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing19_Pyridoxine_HCl -.-> CHEBI_30961
    ing20_Riboflavin["Riboflavin"]
    CultureMech_015460 --> ing20_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing20_Riboflavin -.-> CHEBI_17015
    ing21_biotin["biotin"]
    CultureMech_015460 --> ing21_biotin
    CHEBI_15956["CHEBI:15956"]
    ing21_biotin -.-> CHEBI_15956
    ing22_Thiamine_HCl["Thiamine HCl"]
    CultureMech_015460 --> ing22_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing22_Thiamine_HCl -.-> CHEBI_49105
    ing23_Nicotinic_Acid["Nicotinic Acid"]
    CultureMech_015460 --> ing23_Nicotinic_Acid
    CHEBI_15940["CHEBI:15940"]
    ing23_Nicotinic_Acid -.-> CHEBI_15940
    ing24_calcium_pantothenate["calcium pantothenate"]
    CultureMech_015460 --> ing24_calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing24_calcium_pantothenate -.-> CHEBI_31345
    ing25_Cyanocobalamin["Cyanocobalamin"]
    CultureMech_015460 --> ing25_Cyanocobalamin
    CHEBI_17439["CHEBI:17439"]
    ing25_Cyanocobalamin -.-> CHEBI_17439
    ing26_4_Aminobenzoic_acid["4-Aminobenzoic acid"]
    CultureMech_015460 --> ing26_4_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing26_4_Aminobenzoic_acid -.-> CHEBI_30753
    ing27_Lipoic_acid["Lipoic acid"]
    CultureMech_015460 --> ing27_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing27_Lipoic_acid -.-> CHEBI_16494

Ingredients (28)

#	Ingredient	Concentration	Ontology	Notes
1	Sea salts	20 G_PER_L
2	Ammonium Sulfate 2H4N.O4S 132.141 g/mol	0.3 G_PER_L	CHEBI:62946 ammonium sulfate	Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 7783-20-2; MW: 132.14
3	Potassium phosphate monobasic H2O4P.K 136.084 g/mol	0.1 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 7778-77-0; MW: 136.09
4	EDTA C10H16N2O8 292.244 g/mol	5.0 MG_PER_L	CHEBI:4735 ethylenediaminetetraacetic acid	Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 60-00-4; MW: 292.24; From ATCC Wolfe's mineral mix
5	Magnesium Sulfate Heptahydrate 7H2O.Mg.O4S 246.473 g/mol	3.0 MG_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	CAS: 10034-99-8; MW: 246.47; From ATCC Wolfe's mineral mix
6	Manganese (II) sulfate monohydrate H2O.Mn.O4S 169.016 g/mol	5.0 MG_PER_L	CHEBI:86364 manganese(II) sulfate monohydrate	CAS: 10034-96-5; MW: 169.02; From ATCC Wolfe's mineral mix
7	Sodium Chloride Cl.Na 58.443 g/mol	10.0 MG_PER_L	CHEBI:26710 sodium chloride	CAS: 7647-14-5; MW: 58.44; From ATCC Wolfe's mineral mix
8	Iron (II) sulfate heptahydrate Fe.7H2O.O4S 278.013 g/mol	1.0 MG_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	CAS: 7782-63-0; MW: 278.01; From ATCC Wolfe's mineral mix; Ontology enriched via CAS-RN lookup
9	Cobalt(II) nitrate hexahydrate Co.6H2O.2NO3 291.031 g/mol	1.0 MG_PER_L	CHEBI:86214 cobalt dinitrate hexahydrate	CAS: 10026-22-9; MW: 291.03; From ATCC Wolfe's mineral mix; Ontology enriched via CAS-RN lookup
10	Calcium Chloride Dihydrate Ca.2Cl.2H2O 147.014 g/mol	1.0 MG_PER_L	CHEBI:86158 calcium chloride dihydrate	Mapping: micromediaparam_legacy (confidence: 0.90); From ATCC Wolfe's mineral mix
11	Zinc sulfate heptahydrate 7H2O.O4S.Zn 287.558 g/mol	1.0 MG_PER_L	CHEBI:32312 zinc sulfate heptahydrate	CAS: 7446-20-0; MW: 287.56; From ATCC Wolfe's mineral mix; Ontology enriched via CAS-RN lookup
12	Copper (II) sulfate pentahydrate Cu.5H2O.O4S 249.684 g/mol	0.1 MG_PER_L	CHEBI:31440 copper(II) sulfate pentahydrate	CAS: 7758-99-8; MW: 249.69; From ATCC Wolfe's mineral mix; Ontology enriched via CAS-RN lookup
13	Aluminum potassium sulfate dodecahydrate Al.12H2O.K.2O4S 474.386 g/mol	0.1 MG_PER_L	CHEBI:86465 potassium aluminium sulfate dodecahydrate	CAS: 7784-24-9; MW: 474.39; From ATCC Wolfe's mineral mix; Ontology enriched via CAS-RN lookup
14	Boric Acid H3BO3 61.833 g/mol	0.1 MG_PER_L	CHEBI:33118 boric acid	CAS: 10043-35-3; MW: 61.83; From ATCC Wolfe's mineral mix
15	Sodium Molybdate Dihydrate 2H2O.MoO4.2Na 241.946 g/mol	0.1 MG_PER_L	CHEBI:75213 sodium molybdate dihydrate	CAS: 10102-40-6; MW: 241.95; From ATCC Wolfe's mineral mix; Ontology enriched via CAS-RN lookup
16	Sodium selenite pentahydrate 5H2O.2Na.O3Se 263.012 g/mol	0.01 MG_PER_L	CHEBI:131361 disodium selenite pentahydrate	CAS: 26970-82-1; MW: 263.01; From ATCC Wolfe's mineral mix; Ontology enriched via CAS-RN lookup
17	Sodium tungstate dihydrate 2H2O.2Na.O4W 329.846 g/mol	0.1 MG_PER_L	CHEBI:63939 sodium tungstate dihydrate	CAS: 10213-10-2; MW: 329.85; From ATCC Wolfe's mineral mix; Ontology enriched via CAS-RN lookup
18	Nickel (II) chloride hexahydrate 2Cl.6H2O.Ni 237.689 g/mol	0.2 MG_PER_L	CHEBI:53542 nickel chloride hexahydrate	CAS: 7791-20-0; MW: 237.69; From ATCC Wolfe's mineral mix; Ontology enriched via CAS-RN lookup
19	Folic Acid C19H19N7O6 441.404 g/mol	20.0 MICROG_PER_L	CHEBI:27470 folic acid	Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 59-30-3; MW: 441.4; From ATCC Wolfe's vitamin mix
20	Pyridoxine HCl C8H11NO3.HCl 205.641 g/mol	100.0 MICROG_PER_L	CHEBI:30961 pyridoxine hydrochloride	Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 58-56-0; MW: 205.64; From ATCC Wolfe's vitamin mix
21	Riboflavin C17H20N4O6 376.369 g/mol	50.0 MICROG_PER_L	CHEBI:17015 riboflavin	Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 83-88-5; MW: 376.36; From ATCC Wolfe's vitamin mix
22	biotin C10H16N2O3S 244.316 g/mol	20.0 MICROG_PER_L	CHEBI:15956 biotin	Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 58-85-5; MW: 244.31; From ATCC Wolfe's vitamin mix
23	Thiamine HCl C12H17N4OS.Cl.HCl 337.276 g/mol	50.0 MICROG_PER_L	CHEBI:49105 thiamine hydrochloride	Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 67-03-8; MW: 337.27; From ATCC Wolfe's vitamin mix
24	Nicotinic Acid C6H5NO2 123.111 g/mol	50.0 MICROG_PER_L	CHEBI:15940 nicotinic acid	Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 59-67-6; MW: 123.11; From ATCC Wolfe's vitamin mix
25	calcium pantothenate 2C9H16NO5.Ca 476.536 g/mol	50.0 MICROG_PER_L	CHEBI:31345 Calcium pantothenate	Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 137-08-6; MW: 238.27; From ATCC Wolfe's vitamin mix
26	Cyanocobalamin C63H88CoN14O14P 1355.388 g/mol	1.0 MICROG_PER_L	CHEBI:17439 cyanocob(III)alamin	Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 68-19-9; MW: 1355.37; From ATCC Wolfe's vitamin mix
27	4-Aminobenzoic acid C7H7NO2 137.138 g/mol	50.0 MICROG_PER_L	CHEBI:30753 4-aminobenzoic acid	CAS: 150-13-0; MW: 137.14; From ATCC Wolfe's vitamin mix; Ontology enriched via CAS-RN lookup
28	Lipoic acid C8H14O2S2 206.332 g/mol	50.0 MICROG_PER_L	CHEBI:16494 lipoic acid	Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 1077-28-7; MW: 206.33; From ATCC Wolfe's vitamin mix

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-03-18T04:31:12.668978+00:00	import_from_culturebotht.py	IMPORTED	Imported from CultureBotHT repository: DinoMM_noCarbon_HighNutrient_ATCC.yaml
2026-03-18T04:33:58.430481Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:015460
2026-05-16T09:03:30.844223+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	category->lowercase=1; concentration.unit aliases=10
2026-06-08T23:19:37.851405+00:00	chebi-regrounding-v1.0	Corrected CHEBI ingredient grounding (audit G21/G22)	Re-grounded 1 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).