DinoMM noNitrogen HighNutrient GlucoseC (CultureMech:015462)

Overview

Notes: Source: FEBA media definitions

Composition

flowchart LR
    CultureMech_015462["DinoMM noNitrogen HighNutrient GlucoseC"]
    ing0_Sea_salts["Sea salts"]
    CultureMech_015462 --> ing0_Sea_salts
    ing1_Potassium_phosphate_monobasic["Potassium phosphate monobasic"]
    CultureMech_015462 --> ing1_Potassium_phosphate_monobasic
    CHEBI_63036["CHEBI:63036"]
    ing1_Potassium_phosphate_monobasic -.-> CHEBI_63036
    ing2_D_Glucose["D-Glucose"]
    CultureMech_015462 --> ing2_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing2_D_Glucose -.-> CHEBI_17634
    ing3_Magnesium_Sulfate_Heptahydrate["Magnesium Sulfate Heptahydrate"]
    CultureMech_015462 --> ing3_Magnesium_Sulfate_Heptahydrate
    CHEBI_31795["CHEBI:31795"]
    ing3_Magnesium_Sulfate_Heptahydrate -.-> CHEBI_31795
    ing4_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_015462 --> ing4_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing4_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing5_Sodium_Chloride["Sodium Chloride"]
    CultureMech_015462 --> ing5_Sodium_Chloride
    CHEBI_26710["CHEBI:26710"]
    ing5_Sodium_Chloride -.-> CHEBI_26710
    ing6_Manganese__II__sulfate_monohydrate["Manganese (II) sulfate monohydrate"]
    CultureMech_015462 --> ing6_Manganese__II__sulfate_monohydrate
    CHEBI_86364["CHEBI:86364"]
    ing6_Manganese__II__sulfate_monohydrate -.-> CHEBI_86364
    ing7_Cobalt_chloride_hexahydrate["Cobalt chloride hexahydrate"]
    CultureMech_015462 --> ing7_Cobalt_chloride_hexahydrate
    CHEBI_53503["CHEBI:53503"]
    ing7_Cobalt_chloride_hexahydrate -.-> CHEBI_53503
    ing8_Zinc_sulfate_heptahydrate["Zinc sulfate heptahydrate"]
    CultureMech_015462 --> ing8_Zinc_sulfate_heptahydrate
    CHEBI_32312["CHEBI:32312"]
    ing8_Zinc_sulfate_heptahydrate -.-> CHEBI_32312
    ing9_Calcium_Chloride_Dihydrate["Calcium Chloride Dihydrate"]
    CultureMech_015462 --> ing9_Calcium_Chloride_Dihydrate
    CHEBI_86158["CHEBI:86158"]
    ing9_Calcium_Chloride_Dihydrate -.-> CHEBI_86158
    ing10_Iron__II__sulfate_heptahydrate["Iron (II) sulfate heptahydrate"]
    CultureMech_015462 --> ing10_Iron__II__sulfate_heptahydrate
    CHEBI_75836["CHEBI:75836"]
    ing10_Iron__II__sulfate_heptahydrate -.-> CHEBI_75836
    ing11_Nickel__II__chloride_hexahydrate["Nickel (II) chloride hexahydrate"]
    CultureMech_015462 --> ing11_Nickel__II__chloride_hexahydrate
    CHEBI_53542["CHEBI:53542"]
    ing11_Nickel__II__chloride_hexahydrate -.-> CHEBI_53542
    ing12_Aluminum_potassium_sulfate_dodecahydrate["Aluminum potassium sulfate dodecahydrate"]
    CultureMech_015462 --> ing12_Aluminum_potassium_sulfate_dodecahydrate
    CHEBI_86465["CHEBI:86465"]
    ing12_Aluminum_potassium_sulfate_dodecahydrate -.-> CHEBI_86465
    ing13_Copper__II__sulfate_pentahydrate["Copper (II) sulfate pentahydrate"]
    CultureMech_015462 --> ing13_Copper__II__sulfate_pentahydrate
    CHEBI_31440["CHEBI:31440"]
    ing13_Copper__II__sulfate_pentahydrate -.-> CHEBI_31440
    ing14_Boric_Acid["Boric Acid"]
    CultureMech_015462 --> ing14_Boric_Acid
    CHEBI_33118["CHEBI:33118"]
    ing14_Boric_Acid -.-> CHEBI_33118
    ing15_Sodium_Molybdate_Dihydrate["Sodium Molybdate Dihydrate"]
    CultureMech_015462 --> ing15_Sodium_Molybdate_Dihydrate
    CHEBI_75213["CHEBI:75213"]
    ing15_Sodium_Molybdate_Dihydrate -.-> CHEBI_75213
    ing16_Sodium_selenite_pentahydrate["Sodium selenite pentahydrate"]
    CultureMech_015462 --> ing16_Sodium_selenite_pentahydrate
    CHEBI_131361["CHEBI:131361"]
    ing16_Sodium_selenite_pentahydrate -.-> CHEBI_131361
    ing17_pyridoxine_HCl["pyridoxine HCl"]
    CultureMech_015462 --> ing17_pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing17_pyridoxine_HCl -.-> CHEBI_30961
    ing18_4_Aminobenzoic_acid["4-Aminobenzoic acid"]
    CultureMech_015462 --> ing18_4_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing18_4_Aminobenzoic_acid -.-> CHEBI_30753
    ing19_Lipoic_acid["Lipoic acid"]
    CultureMech_015462 --> ing19_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing19_Lipoic_acid -.-> CHEBI_16494
    ing20_Nicotinic_Acid["Nicotinic Acid"]
    CultureMech_015462 --> ing20_Nicotinic_Acid
    CHEBI_15940["CHEBI:15940"]
    ing20_Nicotinic_Acid -.-> CHEBI_15940
    ing21_Riboflavin["Riboflavin"]
    CultureMech_015462 --> ing21_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing21_Riboflavin -.-> CHEBI_17015
    ing22_thiamine_HCl["thiamine HCl"]
    CultureMech_015462 --> ing22_thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing22_thiamine_HCl -.-> CHEBI_49105
    ing23_calcium_pantothenate["calcium pantothenate"]
    CultureMech_015462 --> ing23_calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing23_calcium_pantothenate -.-> CHEBI_31345
    ing24_biotin["biotin"]
    CultureMech_015462 --> ing24_biotin
    CHEBI_15956["CHEBI:15956"]
    ing24_biotin -.-> CHEBI_15956
    ing25_Folic_Acid["Folic Acid"]
    CultureMech_015462 --> ing25_Folic_Acid
    CHEBI_27470["CHEBI:27470"]
    ing25_Folic_Acid -.-> CHEBI_27470
    ing26_Cyanocobalamin["Cyanocobalamin"]
    CultureMech_015462 --> ing26_Cyanocobalamin
    CHEBI_17439["CHEBI:17439"]
    ing26_Cyanocobalamin -.-> CHEBI_17439

Ingredients (27)

#	Ingredient	Concentration	Ontology	Notes
1	Sea salts	20 G_PER_L
2	Potassium phosphate monobasic H2O4P.K 136.084 g/mol	0.1 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 7778-77-0; MW: 136.09
3	D-Glucose C6H12O6 180.156 g/mol	20 MILLIMOLAR	CHEBI:17634 D-glucose	Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 50-99-7; MW: 180.16
4	Magnesium Sulfate Heptahydrate 7H2O.Mg.O4S 246.473 g/mol	0.03 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	CAS: 10034-99-8; MW: 246.47; From Wolfe's mineral mix
5	Nitrilotriacetic acid C6H9NO6 191.139 g/mol	0.015 G_PER_L	CHEBI:44557 nitrilotriacetic acid	Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 139-13-9; MW: 191.14; From Wolfe's mineral mix
6	Sodium Chloride Cl.Na 58.443 g/mol	0.01 G_PER_L	CHEBI:26710 sodium chloride	CAS: 7647-14-5; MW: 58.44; From Wolfe's mineral mix
7	Manganese (II) sulfate monohydrate H2O.Mn.O4S 169.016 g/mol	0.005 G_PER_L	CHEBI:86364 manganese(II) sulfate monohydrate	CAS: 10034-96-5; MW: 169.02; From Wolfe's mineral mix
8	Cobalt chloride hexahydrate 2Cl.Co.6H2O 237.929 g/mol	0.001 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate	CAS: 7791-13-1; MW: 237.93; From Wolfe's mineral mix; Ontology enriched via CAS-RN lookup
9	Zinc sulfate heptahydrate 7H2O.O4S.Zn 287.558 g/mol	0.001 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate	CAS: 7446-20-0; MW: 287.56; From Wolfe's mineral mix; Ontology enriched via CAS-RN lookup
10	Calcium Chloride Dihydrate Ca.2Cl.2H2O 147.014 g/mol	0.001 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Mapping: micromediaparam_legacy (confidence: 0.90); From Wolfe's mineral mix
11	Iron (II) sulfate heptahydrate Fe.7H2O.O4S 278.013 g/mol	0.001 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	CAS: 7782-63-0; MW: 278.01; From Wolfe's mineral mix; Ontology enriched via CAS-RN lookup
12	Nickel (II) chloride hexahydrate 2Cl.6H2O.Ni 237.689 g/mol	0.00025 G_PER_L	CHEBI:53542 nickel chloride hexahydrate	CAS: 7791-20-0; MW: 237.69; From Wolfe's mineral mix; Ontology enriched via CAS-RN lookup
13	Aluminum potassium sulfate dodecahydrate Al.12H2O.K.2O4S 474.386 g/mol	0.0002 G_PER_L	CHEBI:86465 potassium aluminium sulfate dodecahydrate	CAS: 7784-24-9; MW: 474.39; From Wolfe's mineral mix; Ontology enriched via CAS-RN lookup
14	Copper (II) sulfate pentahydrate Cu.5H2O.O4S 249.684 g/mol	0.0001 G_PER_L	CHEBI:31440 copper(II) sulfate pentahydrate	CAS: 7758-99-8; MW: 249.69; From Wolfe's mineral mix; Ontology enriched via CAS-RN lookup
15	Boric Acid H3BO3 61.833 g/mol	0.0001 G_PER_L	CHEBI:33118 boric acid	CAS: 10043-35-3; MW: 61.83; From Wolfe's mineral mix
16	Sodium Molybdate Dihydrate 2H2O.MoO4.2Na 241.946 g/mol	0.0001 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	CAS: 10102-40-6; MW: 241.95; From Wolfe's mineral mix; Ontology enriched via CAS-RN lookup
17	Sodium selenite pentahydrate 5H2O.2Na.O3Se 263.012 g/mol	0.003 MG_PER_L	CHEBI:131361 disodium selenite pentahydrate	CAS: 26970-82-1; MW: 263.01; From Wolfe's mineral mix; Ontology enriched via CAS-RN lookup
18	pyridoxine HCl C8H11NO3.HCl 205.641 g/mol	0.1 MG_PER_L	CHEBI:30961 pyridoxine hydrochloride	Mapping: micromediaparam_legacy (confidence: 0.90); From Wolfe's vitamin mix
19	4-Aminobenzoic acid C7H7NO2 137.138 g/mol	0.05 MG_PER_L	CHEBI:30753 4-aminobenzoic acid	CAS: 150-13-0; MW: 137.14; From Wolfe's vitamin mix; Ontology enriched via CAS-RN lookup
20	Lipoic acid C8H14O2S2 206.332 g/mol	0.05 MG_PER_L	CHEBI:16494 lipoic acid	Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 1077-28-7; MW: 206.33; From Wolfe's vitamin mix
21	Nicotinic Acid C6H5NO2 123.111 g/mol	0.05 MG_PER_L	CHEBI:15940 nicotinic acid	Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 59-67-6; MW: 123.11; From Wolfe's vitamin mix
22	Riboflavin C17H20N4O6 376.369 g/mol	0.05 MG_PER_L	CHEBI:17015 riboflavin	Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 83-88-5; MW: 376.36; From Wolfe's vitamin mix
23	thiamine HCl C12H17N4OS.Cl.HCl 337.276 g/mol	0.05 MG_PER_L	CHEBI:49105 thiamine hydrochloride	Mapping: micromediaparam_legacy (confidence: 0.90); From Wolfe's vitamin mix
24	calcium pantothenate 2C9H16NO5.Ca 476.536 g/mol	0.05 MG_PER_L	CHEBI:31345 Calcium pantothenate	Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 137-08-6; MW: 238.27; From Wolfe's vitamin mix
25	biotin C10H16N2O3S 244.316 g/mol	0.02 MG_PER_L	CHEBI:15956 biotin	Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 58-85-5; MW: 244.31; From Wolfe's vitamin mix
26	Folic Acid C19H19N7O6 441.404 g/mol	0.02 MG_PER_L	CHEBI:27470 folic acid	Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 59-30-3; MW: 441.4; From Wolfe's vitamin mix
27	Cyanocobalamin C63H88CoN14O14P 1355.388 g/mol	0.001 MG_PER_L	CHEBI:17439 cyanocob(III)alamin	Mapping: micromediaparam_legacy (confidence: 0.90); CAS: 68-19-9; MW: 1355.37; From Wolfe's vitamin mix

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-03-18T04:31:12.701942+00:00	import_from_culturebotht.py	IMPORTED	Imported from CultureBotHT repository: DinoMM_noNitrogen_HighNutrient_GlucoseC.yaml
2026-03-18T04:33:58.452750Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:015462
2026-05-16T09:03:30.868669+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	category->lowercase=1
2026-06-08T23:19:37.876499+00:00	chebi-regrounding-v1.0	Corrected CHEBI ingredient grounding (audit G21/G22)	Re-grounded 1 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:56.404549+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.