methanoculleus_7t_medium

Overview

External ontology

jcm.grmd:1456 (source link unavailable) — METHANOCULLEUS 7T MEDIUM

Applications

• Microbial cultivation

pH

7.3

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1456

Composition

flowchart LR
    CultureMech_015888["methanoculleus_7t_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_015888 --> ing0_KH2PO4
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_015888 --> ing1_MgSO4_7H2O
    ing2_NaCl["NaCl"]
    CultureMech_015888 --> ing2_NaCl
    ing3_NH4Cl["NH4Cl"]
    CultureMech_015888 --> ing3_NH4Cl
    ing4_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_015888 --> ing4_CaCl2_2H2O
    ing5_FeSO4_7H2O["FeSO4·7H2O"]
    CultureMech_015888 --> ing5_FeSO4_7H2O
    ing6_FeCl2_solution__see_Medium_No__187__["FeCl2 solution (see Medium No. 187 )"]
    CultureMech_015888 --> ing6_FeCl2_solution__see_Medium_No__187__
    ing7_Trace_element_solution__see_Medium_No__187__["Trace element solution (see Medium No. 187 )"]
    CultureMech_015888 --> ing7_Trace_element_solution__see_Medium_No__187__
    ing8_Trace_vitamin__see_Medium_No__197__["Trace vitamin (see Medium No. 197 )"]
    CultureMech_015888 --> ing8_Trace_vitamin__see_Medium_No__197__
    ing9_Sodium_acetate["Sodium acetate"]
    CultureMech_015888 --> ing9_Sodium_acetate
    ing10_Na2WO4_2H2O["Na2WO4·2H2O"]
    CultureMech_015888 --> ing10_Na2WO4_2H2O
    ing11_NaHCO3["NaHCO3"]
    CultureMech_015888 --> ing11_NaHCO3
    ing12_Resazurin["Resazurin"]
    CultureMech_015888 --> ing12_Resazurin
    ing13_Distilled_water["Distilled water"]
    CultureMech_015888 --> ing13_Distilled_water

Ingredients (14)

Preparation

1. Mix components, except NaHCO3, and bring to a boil for 5-10 seconds.
2. Cool down under a stream of N2-CO2 (80:20 v/v), and add NaHCO3 to the medium.
3. Adjust pH to 7.3 -7.6.
4. Dispense the medium into culture vessels under a stream of H2-CO2 (80:20 v/v), seal with butyl rubber stoppers and autoclave.
5. Prior to inoculation, add per liter the following solutions (autoclaved and stored under a N2 atmosphere).
6. Pressurize inoculated vessels to 100 kPa with a H2-CO2 (80:20 v/v) gas mixture.

Target organisms

No target organisms are recorded.

Provenance