methanococcus_cf_medium

Overview

External ontology

jcm.grmd:1464 (source link unavailable) — METHANOCOCCUS CF MEDIUM

Applications

• Microbial cultivation

pH

6.5

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1464

Composition

flowchart LR
    CultureMech_015889["methanococcus_cf_medium"]
    ing0_KCl["KCl"]
    CultureMech_015889 --> ing0_KCl
    ing1_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_015889 --> ing1_MgCl2_6H2O
    ing2_NH4Cl["NH4Cl"]
    CultureMech_015889 --> ing2_NH4Cl
    ing3_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_015889 --> ing3_CaCl2_2H2O
    ing4_K2HPO4["K2HPO4"]
    CultureMech_015889 --> ing4_K2HPO4
    ing5_NaCl["NaCl"]
    CultureMech_015889 --> ing5_NaCl
    ing6_Trace_mineral_solution__see_Medium_No__151__["Trace mineral solution (see Medium No. 151 )"]
    CultureMech_015889 --> ing6_Trace_mineral_solution__see_Medium_No__151__
    ing7_Ni_Se_W_solution__see_Medium_No__1462__["Ni-Se-W solution (see Medium No. 1462 )"]
    CultureMech_015889 --> ing7_Ni_Se_W_solution__see_Medium_No__1462__
    ing8_Fe_NH4_2_SO4_2_6H2O["Fe(NH4)2(SO4)2·6H2O"]
    CultureMech_015889 --> ing8_Fe_NH4_2_SO4_2_6H2O
    ing9_NaHCO3["NaHCO3"]
    CultureMech_015889 --> ing9_NaHCO3
    ing10_Sodium_acetate["Sodium acetate"]
    CultureMech_015889 --> ing10_Sodium_acetate
    ing11_Resazurin["Resazurin"]
    CultureMech_015889 --> ing11_Resazurin
    ing12_Distilled_water["Distilled water"]
    CultureMech_015889 --> ing12_Distilled_water

Ingredients (13)

Preparation

1. Mix components thoroughly and adjust pH to 6.5, then distribute the medium into culture vessels under a H2-CO2 (80:20, v/v) gas mixture and seal with butyl rubber stoppers.
2. After autoclaving, stand the medium overnight.
3. Prior to use, add per liter the following solutions (autoclaved or* filter-sterilized, and stored under a N2 atmosphere): Adjust pH to 6.8 - 7.0 if necessary.
4. Pressurize the inoculated vessels to 200 kPa H2-CO2 (80:20, v/v).

Target organisms

No target organisms are recorded.

Provenance