fcgm_hepes (CultureMech:015894)

Overview

Applications

Protozoa cultivation

pH

7.0

Notes

CCAP prepares this buffered FCGM variant by making 50 mM HEPES in deionised water at pH 7.0, then boiling, steeping, filtering, and autoclaving Ward's cereal grass leaves in that buffer.

Composition

flowchart LR
    CultureMech_015894["fcgm_hepes"]
    ing0_Deionised_water["Deionised water"]
    CultureMech_015894 --> ing0_Deionised_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Deionised_water -.-> CHEBI_15377
    ing1_HEPES["HEPES"]
    CultureMech_015894 --> ing1_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing1_HEPES -.-> CHEBI_46756
    ing2_Cereal_grass_leaves["Cereal grass leaves"]
    CultureMech_015894 --> ing2_Cereal_grass_leaves

Ingredients (3)

#	Ingredient	Concentration	Ontology	Notes
1	Deionised water H2O 18.015 g/mol	1000 ML_PER_L	CHEBI:15377 water	CCAP lists 500 mL deionised water per 500 mL medium.
2	HEPES	11.9 G_PER_L	CHEBI:46756 HEPES	CCAP lists 5.95 g HEPES per 500 mL medium to make a 50 mM HEPES solution.
3	Cereal grass leaves	1.25 G_PER_L		Ward's cereal grass leaves; CCAP lists 0.625 g per 500 mL medium.

Preparation

Make a 50 mM HEPES solution with deionised water.
Adjust the HEPES solution to pH 7.0 with 1 M NaOH or 1 M HCl.
Bring the pH-adjusted HEPES solution to the boil.
Add the cereal grass leaves, boil for 5 minutes, then leave to steep for 3 hours.
Filter through a Buchner funnel.
Autoclave at 15 psi for 15 minutes.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-10-01T02:46:22+00:00	codex-agent	ADDED_CCAP_MISSING_ALGAE_MEDIUM	Live CCAP index advertised FCGM and the exact CCAP FCGM + HEPES formulation was absent from data/normalized_yaml before this add-only curation pass.
2026-10-01T02:55:00+00:00	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:015894