Overview
- External ontology
- nbrc.medium:281 (source link unavailable) — NBRC Medium 281
- Applications
-
- Microbial cultivation
- pH
- 3.0
- Notes
- Source: NBRC | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=281
Composition
flowchart LR
CultureMech_015899["nbrc_medium_281"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_015899 --> ing0_Yeast_extract
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_015899 --> ing1__NH4_2SO4
ing2_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_015899 --> ing2_CaCl2_2H2O
ing3_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_015899 --> ing3_MgSO4_7H2O
ing4_KH2PO4["KH2PO4"]
CultureMech_015899 --> ing4_KH2PO4
ing5_Na2SiO3_9H2O["Na2SiO3·9H2O"]
CultureMech_015899 --> ing5_Na2SiO3_9H2O
ing6_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_015899 --> ing6_Na2MoO4_2H2O
ing7_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_015899 --> ing7_FeSO4_7H2O
ing8_Distilled_water["Distilled water"]
CultureMech_015899 --> ing8_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing8_Distilled_water -.-> CHEBI_15377
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 1 G_PER_L | ||
| 2 | (NH4)2SO4 | 1.3 G_PER_L | ||
| 3 | CaCl2·2H2O | 0.08 G_PER_L | ||
| 4 | MgSO4·7H2O | 0.25 G_PER_L | ||
| 5 | KH2PO4 | 0.28 G_PER_L | ||
| 6 | Na2SiO3·9H2O | 0.3 G_PER_L | ||
| 7 | Na2MoO4·2H2O | 0.03 G_PER_L | ||
| 8 | FeSO4·7H2O | 0.02 G_PER_L | ||
| 9 |
Distilled water
H2O 18.015 g/mol
|
1 L |
CHEBI:15377
water
|
Preparation
- Adjust pH to 3.0 with 10N H2SO4
- For solid medium, equal volumes of separately autoclaved double-strength liquid medium (pH 2.0) and 1.6% Gelrite (Kelco, Division of Merck & Co, Inc.) are mixed, after cooling to 45C.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-10-02T05:20:01.508978+00:00 | nbrc-live-import | Scraped from NBRC catalogue | Direct import from NBRC Medium 281. Ingredients captured verbatim from the live catalogue. |
| 2026-10-01T03:40:36.654536+00:00 | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:015899 |