Overview
- External ontology
- nbrc.medium:367 (source link unavailable) — mjYPGS Medium
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.5, 'max': 8.0}
- Notes
- Source: NBRC | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=367
Composition
flowchart LR
CultureMech_015902["mjypgs_medium_nbrc_367"]
ing0_NaCl["NaCl"]
CultureMech_015902 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_K2HPO4["K2HPO4"]
CultureMech_015902 --> ing1_K2HPO4
ing2_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_015902 --> ing2_CaCl2_2H2O
ing3_NH4Cl["NH4Cl"]
CultureMech_015902 --> ing3_NH4Cl
ing4_NaNO3["NaNO3"]
CultureMech_015902 --> ing4_NaNO3
ing5_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_015902 --> ing5_MgSO4_7H2O
ing6_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_015902 --> ing6_MgCl2_6H2O
ing7_KCl["KCl"]
CultureMech_015902 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_015902 --> ing8_NiCl2_6H2O
ing9_Na2SeO3_5H2O["Na2SeO3·5H2O"]
CultureMech_015902 --> ing9_Na2SeO3_5H2O
ing10_Ferric_citrate["Ferric citrate"]
CultureMech_015902 --> ing10_Ferric_citrate
ing11_Trace_mineral_solution["Trace mineral solution"]
CultureMech_015902 --> ing11_Trace_mineral_solution
ing12_Na2S2O3_5H2O["Na2S2O3·5H2O"]
CultureMech_015902 --> ing12_Na2S2O3_5H2O
ing13_Na2SiO3_9H2O["Na2SiO3·9H2O"]
CultureMech_015902 --> ing13_Na2SiO3_9H2O
ing14_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_015902 --> ing14_Bacto_Yeast_Extract__Difco_
ing15_Peptone__BBL_["Peptone (BBL)"]
CultureMech_015902 --> ing15_Peptone__BBL_
ing16_Glucose["Glucose"]
CultureMech_015902 --> ing16_Glucose
CHEBI_17234["CHEBI:17234"]
ing16_Glucose -.-> CHEBI_17234
ing17_Sodium_succinate["Sodium succinate"]
CultureMech_015902 --> ing17_Sodium_succinate
ing18_Distilled_water["Distilled water"]
CultureMech_015902 --> ing18_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing18_Distilled_water -.-> CHEBI_15377
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
3 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 | K2HPO4 | 14 MG_PER_L | ||
| 3 | CaCl2·2H2O | 14 MG_PER_L | ||
| 4 | NH4Cl | 12.5 MG_PER_L | ||
| 5 | NaNO3 | 12.5 MG_PER_L | ||
| 6 | MgSO4·7H2O | 0.34 G_PER_L | ||
| 7 | MgCl2·6H2O | 41.8 MG_PER_L | ||
| 8 |
KCl
Cl.K 74.551 g/mol
|
33 MG_PER_L |
CHEBI:32588
potassium chloride
|
|
| 9 | NiCl2·6H2O | 0.05 MG_PER_L | ||
| 10 | Na2SeO3·5H2O | 0.05 MG_PER_L | ||
| 11 | Ferric citrate | 5 MG_PER_L | ||
| 12 | Trace mineral solution | 1 ML_PER_L | ||
| 13 | Na2S2O3·5H2O | 2 G_PER_L | ||
| 14 | Na2SiO3·9H2O | 1 G_PER_L | ||
| 15 | Bacto Yeast Extract (Difco) | 1 G_PER_L | ||
| 16 | Peptone (BBL) | 1 G_PER_L | ||
| 17 |
Glucose
C6H12O6 180.156 g/mol
|
0.2 G_PER_L |
CHEBI:17234
glucose
|
|
| 18 | Sodium succinate | 0.2 G_PER_L | ||
| 19 |
Distilled water
H2O 18.015 g/mol
|
1 L |
CHEBI:15377
water
|
Preparation
- Adjust pH 7.5 - 8.0 with H2SO4
Solutions
Trace mineral solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Nitrilotriacetic acid | 1.5 G_PER_L | |
| MnSO4·2H2O | 0.5 G_PER_L | |
| CoSO4·7H2O | 0.5 G_PER_L | |
| ZnSO4·7H2O | 0.18 G_PER_L | |
| CuSO4·5H2O | 0.01 MG_PER_L | |
| KAl(SO4)2·12H2O | 0.02 G_PER_L | |
| H3BO3 | 0.01 G_PER_L | |
| Na2MoO4·2H2O | 0.01 MG_PER_L | |
| Distilled water | 1 L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-10-02T05:20:01.508978+00:00 | nbrc-live-import | Scraped from NBRC catalogue | Direct import from NBRC Medium 367. Ingredients captured verbatim from the live catalogue. |
| 2026-10-01T03:40:36.654536+00:00 | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:015902 |