thermoproteus_tenax_medium_nbrc_835

Overview

External ontology

nbrc.medium:835 (source link unavailable) — Thermoproteus tenax Medium

Applications

• Microbial cultivation

pH

5.5

Notes

Source: NBRC | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=835

Composition

flowchart LR
    CultureMech_015903["thermoproteus_tenax_medium_nbrc_835"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_015903 --> ing0__NH4_2SO4
    ing1_FeSO4_7H2O["FeSO4·7H2O"]
    CultureMech_015903 --> ing1_FeSO4_7H2O
    ing2_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_015903 --> ing2_MgSO4_7H2O
    ing3_CaSO4_2H2O["CaSO4·2H2O"]
    CultureMech_015903 --> ing3_CaSO4_2H2O
    ing4_KH2PO4["KH2PO4"]
    CultureMech_015903 --> ing4_KH2PO4
    ing5_NaF["NaF"]
    CultureMech_015903 --> ing5_NaF
    ing6_MnCl2_4H2O["MnCl2·4H2O"]
    CultureMech_015903 --> ing6_MnCl2_4H2O
    ing7_Na2B4O7_10H2O["Na2B4O7·10H2O"]
    CultureMech_015903 --> ing7_Na2B4O7_10H2O
    ing8_ZnSO4_7H2O["ZnSO4·7H2O"]
    CultureMech_015903 --> ing8_ZnSO4_7H2O
    ing9_CuCl2_2H2O["CuCl2·2H2O"]
    CultureMech_015903 --> ing9_CuCl2_2H2O
    ing10_Na2MoO7_2H2O["Na2MoO7·2H2O"]
    CultureMech_015903 --> ing10_Na2MoO7_2H2O
    ing11_CoSO4_7H2O["CoSO4·7H2O"]
    CultureMech_015903 --> ing11_CoSO4_7H2O
    ing12_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
    CultureMech_015903 --> ing12_Bacto_Yeast_Extract__Difco_
    ing13_Soluble_starch["Soluble starch"]
    CultureMech_015903 --> ing13_Soluble_starch
    CHEBI_28017["CHEBI:28017"]
    ing13_Soluble_starch -.-> CHEBI_28017
    ing14_Sulfur__powder_["Sulfur (powder)"]
    CultureMech_015903 --> ing14_Sulfur__powder_
    CHEBI_26833["CHEBI:26833"]
    ing14_Sulfur__powder_ -.-> CHEBI_26833
    ing15_Na2S_9H2O["Na2S·9H2O"]
    CultureMech_015903 --> ing15_Na2S_9H2O
    ing16_Resazurin["Resazurin"]
    CultureMech_015903 --> ing16_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing16_Resazurin -.-> CHEBI_8806
    ing17_Distilled_water["Distilled water"]
    CultureMech_015903 --> ing17_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing17_Distilled_water -.-> CHEBI_15377

Ingredients (18)

Preparation

1. Mix ingredients except yeast extract, starch, sulfur and Na2S·9H2O, and filter-sterilize while gassing with N2. Autoclave yeast extract and starch as 10% solutions and Na2S·9H2O as a 5% solution under a N2 atmosphere. Sulfur is sterilized separately by autoclaving for 60 min at 105C on each of 3 successive days. Aseptically and anaerobically add the yeast extract and starch solutions, and sulfur to the medium. Prior to inoculation, add the sterile Na2S·9H2O solution, and adjust pH to 5.5 with sterile 1 N H2SO4.

Target organisms

No target organisms are recorded.

Provenance