chloracidobacterium_thermophilum_midnight_medium_ctm (CultureMech:015911)

Overview

External ontology

mediadive.medium:1783 (source link unavailable) — Chloracidobacterium thermophilum Midnight Medium (CTM-Medium)

Applications

Microbial cultivation

pH

7.0

Notes

Source: MediaDive | Link: https://mediadive.dsmz.de/rest/medium/1783

Composition

flowchart LR
    CultureMech_015911["chloracidobacterium_thermophilum_midnight_medium_ctm"]
    ing0_Solution_1["Solution 1"]
    CultureMech_015911 --> ing0_Solution_1
    ing1_Solution_2["Solution 2"]
    CultureMech_015911 --> ing1_Solution_2
    ing2_Solution_3["Solution 3"]
    CultureMech_015911 --> ing2_Solution_3
    ing3_Solution_4["Solution 4"]
    CultureMech_015911 --> ing3_Solution_4
    ing4_HEPES_buffer["HEPES buffer"]
    CultureMech_015911 --> ing4_HEPES_buffer
    ing5_NaHCO3["NaHCO3"]
    CultureMech_015911 --> ing5_NaHCO3
    ing6_Na_thioglycolate["Na-thioglycolate"]
    CultureMech_015911 --> ing6_Na_thioglycolate
    ing7_Solution_5["Solution 5"]
    CultureMech_015911 --> ing7_Solution_5
    ing8_Solution_6["Solution 6"]
    CultureMech_015911 --> ing8_Solution_6
    ing9_Vitamin_solution_A__1000_x_stock_["Vitamin solution A (1000 x stock)"]
    CultureMech_015911 --> ing9_Vitamin_solution_A__1000_x_stock_
    ing10_Vitamin_solution_B__1000_x_stock_["Vitamin solution B (1000 x stock)"]
    CultureMech_015911 --> ing10_Vitamin_solution_B__1000_x_stock_

Ingredients (11)

#	Ingredient	Concentration	Ontology	Notes
1	Solution 1	20 ML_PER_L
2	Solution 2	3 ML_PER_L
3	Solution 3	2 ML_PER_L
4	Solution 4	2.5 ML_PER_L
5	HEPES buffer	2.4 G_PER_L	mediadive.compound:461 (source link unavailable) HEPES buffer
6	NaHCO3	0.625 G_PER_L	mediadive.compound:55 (source link unavailable) NaHCO3	or 10 mL of a 6.25% solution in water
7	Na-thioglycolate	0.125 G_PER_L	mediadive.compound:30 (source link unavailable) Na-thioglycolate	or use 1 mL of a 12.5% solution in water, autoclaved under a N2 gasphase
8	Solution 5	0.5 ML_PER_L
9	Solution 6	20 ML_PER_L
10	Vitamin solution A (1000 x stock)	0.25 ML_PER_L
11	Vitamin solution B (1000 x stock)	0.25 ML_PER_L

Preparation

The CTM-Medium consists of two parts. Part one is autoclavable and called CTM-Medium basis. Part two is named mixed solution and contains non-autoclavable components. Solid CTM-Medium contains 1% three times washed Bactoagar and is added to the CTM-Medium basis prior autoclaving.
Note: Bacto peptone can be omitted with only using amino acids as carbon source, which will maker it a defined medium. Amount of amino acids will then have to be increased to 100mg/L each.
Make up to 970 ml with distilled water.
Adjust pH to 7.0 with NaOH (2M).
Autoclave at 121 °C for 20 min.
Seal medium bottle immediately after autoclaving in order to avoid oxygenation.
Cool down to 70 °C (in a water bath).
Add mixed solution to the CTM basis for completing the CTM-Medium (30 mL),
Note: Always prepare freshly! Mix the following components (e.g., in a volume of approx. 30 mL) and add the filter sterilized mixture to the autoclaved CTM-Medium basis:
Keep CTM-Medium tightly sealed and between 50-70˚C prior usage.
It is strongly recommended to use up the CTM-Medium as soon as possible after preparation! Short term storage of CTM-Medium basis possible under strictly anaerobic conditions. Mixed solutions should then be added freshly before use.
Pour CTM-Medium into flasks, bottles or similar and leave a quarter air headspace and seal them.

Solutions

Solution 1

Dose: 20 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
MgSO4 x 7 H2O	3.75 G_PER_L
CaCl2 x 2 H2O	1.8 G_PER_L
Citric acid	0.3 G_PER_L
Na-EDTA x 2 H2O	5.04309 G_PER_L	pH 8, 0,25 M

Trace metal solution

Dose: 50 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
H3BO3	2.86 G_PER_L
MnCl2 x 4 H2O	1.81 G_PER_L
ZnSO4 x 7 H2O	0.222 G_PER_L
Na2MoO4 x 2 H2O	0.39 G_PER_L
CuSO4 x 5 H2O	0.079 G_PER_L
Co(NO3)2 x 6 H2O	0.049 G_PER_L
Distilled water	1000 ML_PER_L

Solution 2

Dose: 3 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
K2HPO4	15.3 G_PER_L

Solution 3

Dose: 2 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Ferric ammonium citrate	11.9522 G_PER_L

Solution 4

Dose: 2.5 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
2-Oxoglutarate	14.6 G_PER_L

Solution 5

Dose: 0.5 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Bacto peptone	100 G_PER_L	BD-Difco
Distilled water	1000 ML_PER_L

Solution 6

Dose: 20 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
L-Alanine	0.25 G_PER_L
L-Arginine	0.25 G_PER_L
L-Asparagine	0.25 G_PER_L
L-Aspartic acid	0.25 G_PER_L
L-Cysteine	0.25 G_PER_L
L-Glutamic acid	0.25 G_PER_L
L-Glutamine	0.25 G_PER_L
Glycine	0.25 G_PER_L
L-Histidine	0.25 G_PER_L
L-Isoleucine	0.25 G_PER_L
L-Leucine	0.25 G_PER_L
L-Lysine	0.25 G_PER_L
L-Methionine	0.25 G_PER_L
L-Phenylalanine	0.25 G_PER_L
L-Proline	0.25 G_PER_L
L-Serine	0.25 G_PER_L
L-Threonine	0.25 G_PER_L
L-Tryptophan	0.25 G_PER_L
L-Tyrosine	0.25 G_PER_L
L-Valine	0.25 G_PER_L
Distilled water	1000 ML_PER_L

Vitamin solution A (1000 x stock)

Dose: 0.25 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Biotin	0.1 G_PER_L
Riboflavin	0.1 G_PER_L
Thiamine HCl	1 G_PER_L
Thiamine pyrophosphate	1 G_PER_L
L-Ascorbic acid	1 G_PER_L
Calcium pantothenate	1 G_PER_L
Folic acid	1 G_PER_L
Nicotinamide	1 G_PER_L
Nicotinic acid	1 G_PER_L
p-Aminobenzoic acid	1 G_PER_L
Pyridoxine hydrochloride	1 G_PER_L
Lipoic acid	1 G_PER_L
beta-NAD	1 G_PER_L
Potassium phosphate buffer	1000 ML_PER_L	pH 7.2, 10 mM

Vitamin solution B (1000 x stock)

Dose: 0.25 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Vitamin B12	1 G_PER_L
Distilled water	1000 ML_PER_L

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-10-01T04:00:20.760278+00:00	mediadive-live-gap-import	Imported from live MediaDive API	Direct import from MediaDive medium 1783. Recipe rows captured from the live API detail payload.
2026-10-01T04:04:39Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:015911