All families / PF02580
PF02580: Tyr_Deacylase
D-Tyr-tRNA(Tyr) deacylase
- Curation status
- SEEDED
- Seed status
- EX DUF
- Characterization
- UNSCORED
Grounding
PF02580: D-Tyr-tRNA(Tyr) deacylase
IPR003732: integrated entry for D-Tyr-tRNA(Tyr) deacylase
Source description
This family comprises of several D-Tyr-tRNA(Tyr) deacylase proteins. Cell growth inhibition by several d-amino acids can be explained by an in vivo production of d-aminoacyl-tRNA molecules. Escherichia coli and yeast cells express an enzyme, d-Tyr-tRNA(Tyr) deacylase, capable of recycling such d-aminoacyl-tRNA molecules into free tRNA and d-amino acid. Accordingly, upon inactivation of the genes of the above deacylases, the toxicity of d-amino acids increases. Orthologues of the deacylase are found in many cells [InterPro reference PUB00017393].The D-aminoacyl-tRNA deacylase (DTD) enzyme is homodimeric with two active sites located at the dimeric interface. Each active site carries an invariant Gly-cisPro dipeptide motif in each monomer. The interaction between the dipeptide motifs from each monomer ensures substrate stereospecificity. This family also includes a subclass of DTDs which is present in Chordata and harbors a Gly-transPro motif. The cis to trans switch is the key to Animal DTDs (ATD) gaining of L-chiral selectivity. This 'gain of function' through relaxation of substrate chiral specificity underlies ATD's capability of correcting the error in tRNA selection [InterPro reference PUB00109228].
Evidence and references
- InterPro entry and its literature references. PUB identifiers are internal InterPro references, not PubMed identifiers. Unresolved citations link to the originating entry.
- Frozen description source: InterPro/Pfam API
Source descriptions and links are provenance, not independent functional validation.
Evidence counts: not scored.
Curated functional assertions
No curated functional assertions supplied.
Protein sequence examples
9 verified sequence examples. FASTA
| UniProt | Protein / organism | Sequence | Provenance |
|---|---|---|---|
| O32042 | Inactive D-aminoacyl-tRNA deacylase Bacillus subtilis (strain 168) | 132 aa; version 3 UniProt 2026_03 | PTM REGISTRY MIRROR11 sibling references
|
| P0A6M4 | D-aminoacyl-tRNA deacylase Escherichia coli (strain K12) | 145 aa; version 1 UniProt 2026_03 | PTM REGISTRY MIRROR14 sibling references
|
| Q07648 | D-aminoacyl-tRNA deacylase Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | 150 aa; version 1 UniProt 2026_03 | UNIPROT FETCH9 sibling references |
| Q8BHA3 | D-aminoacyl-tRNA deacylase 2 Mus musculus | 168 aa; version 1 UniProt 2026_03 | UNIPROT FETCH |
| Q8IIS0 | D-aminoacyl-tRNA deacylase Plasmodium falciparum (isolate 3D7) | 164 aa; version 1 UniProt 2026_03 | UNIPROT FETCH |
| Q8TEA8 | D-aminoacyl-tRNA deacylase 1 Homo sapiens | 209 aa; version 2 UniProt 2026_03 | UNIPROT FETCH |
| Q96FN9 | D-aminoacyl-tRNA deacylase 2 Homo sapiens | 168 aa; version 1 UniProt 2026_03 | UNIPROT FETCH |
| Q9DD18 | D-aminoacyl-tRNA deacylase 1 Mus musculus | 209 aa; version 2 UniProt 2026_03 | UNIPROT FETCH |
| Q9VGP0 | D-aminoacyl-tRNA deacylase Drosophila melanogaster | 158 aa; version 2 UniProt 2026_03 | UNIPROT FETCH2 sibling references |
Pfam membership is not functional evidence. Whole-protein sequences do not establish domain boundaries. A coverage gap here is not absence from UniProt.
Sequences and metadata: UniProt Consortium, CC BY 4.0.
Domain matches
No residue-level domain match evidence is available in this build. Structure and AlphaFold counters do not supply coordinates or establish function.
Snapshot counters
- proteins
- 19,377
- matches
- 19,510
- proteomes
- 17,291
- taxa
- 28,568
- structures
- 22
- alphafold models
- 13,734
- domain architectures
- 110
Classification basis
pfam previous unknown name
No scoring reasons supplied.
Related Mech records
This family joined the worklist after the frozen cross-Mech scan, so other Mechs were not searched for it. Missing links here are not evidence that other Mechs lack it; rows shown come from mentions of a former DUF/UPF name.
No cross-Mech examples are present in the supplied snapshot.
Curation history and reviews
No retained curation events supplied.
No retained review supplied.
Record source
Status and evidence
- Seed status
- A name-based classification of frozen InterPro/Pfam metadata. UNKNOWN CANDIDATE means the metadata explicitly says the function is unknown. KNOWN HISTORICAL DUF means a DUF name matched without that wording; this heuristic is not proof of a known function and does not establish experimental characterization. EX DUF means Pfam renamed the family away from a DUF/UPF name, as recorded in Pfam's previous identifiers; a rename usually follows published work on some members but is naming history, not proof of function for every member. Some EX DUF families' current names or descriptions still say the function is unknown; their classification basis lists this, and scoring does not treat them as characterized. FALSE POSITIVE TEXT HIT means the naming rules did not match.
- Characterization
- A separate evidence-scoring result. UNSCORED means no score has been calculated; it is not evidence that the family lacks a known function. Missing scores are not negative evidence. PARTIALLY CHARACTERIZED has partial support; KNOWN HISTORICAL DUF can inherit the seed label or reflect a known-function signal. Neither label implies experimental validation.
- Evidence counts
- Source-specific signals, not unique proteins, publications, or confidence scores. Known counts Rhea reactions and experimentally supported GO molecular-function annotations. Partial counts functional/domain assignments. Context counts structural, environmental, orthology, interaction and neighborhood signals; context alone does not assign function. Repeated annotations may not be independent.
- Curation status
- Reported separately from seed classification and characterization. SNAPSHOT ONLY means no curated metadata was supplied for this build. SEEDED denotes a source-derived record, not an expert-reviewed functional claim.
- Missing counts
- Not available means the source did not supply that counter. A zero is a reported count. Not scored means evidence counts are unavailable.