PF03781: FGE-sulfatase

Grounding

PF03781: Sulfatase-modifying factor enzyme 1-like domain
IPR005532: integrated entry for Sulfatase-modifying factor enzyme 1-like domain

Source description

This domain is found in eukaryotic proteins [InterPro reference PUB00019471] required for post-translational sulfatase modification (SUMF1). These proteins are associated with the rare disorder multiple sulfatase deficiency (MSD) [InterPro reference PUB00033658]. The protein product of the SUMF1 gene is FGE, formylglycine (FGly),-generating enzyme, which is a sulfatase. Sulfatases are enzymes essential for degradation and remodelling of sulfate esters, and formylglycine (FGly), the key catalytic in the active site, is unique to sulfatases [InterPro reference PUB00038408]. FGE is localised to the endoplasmic reticulum (ER) and interacts with and modifies the unfolded form of newly synthesised sulfatases. FGE is a single-domain monomer with a surprising paucity of secondary structure that adopts a unique fold which is stabilised by two Ca2+ ions. The effect of all mutations found in MSD patients is explained by the FGE structure, providing a molecular basis for MSD. A redox-active disulfide bond is present in the active site of FGE. An oxidised cysteine residue, possibly cysteine sulfenic acid, has been detected that may allow formulation of a structure-based mechanism for FGly formation from cysteine residues in all sulfatases [InterPro reference PUB00055960]. In Mycobacteria and Treponema denticola this enzyme functions as an iron(II)-dependent oxidoreductase [InterPro reference PUB00060032,InterPro reference PUB00066766]. This domain is also found in the Serine/threonine-protein kinase Pkn1 from Chlamydia trachomatis which is a multidomain protein. Pkn1 plays a role in specific interactions with host proteins during host intracellular growth.

Evidence and references

• InterPro entry and its literature references. PUB identifiers are internal InterPro references, not PubMed identifiers. Unresolved citations link to the originating entry.
• Frozen description source: InterPro/Pfam API

Source descriptions and links are provenance, not independent functional validation.

Evidence counts: not scored.

Curated functional assertions

No curated functional assertions supplied.

Protein sequence examples

11 verified sequence examples. FASTA

First 10 shown; the FASTA and family JSON contain all examples.

Pfam membership is not functional evidence. Whole-protein sequences do not establish domain boundaries. A coverage gap here is not absence from UniProt.

Sequences and metadata: UniProt Consortium, CC BY 4.0.

Domain matches

No residue-level domain match evidence is available in this build. Structure and AlphaFold counters do not supply coordinates or establish function.

Snapshot counters

proteins

38,188

matches

41,371

proteomes

14,430

taxa

23,526

structures

65

alphafold models

25,146

domain architectures

587

Classification basis

pfam previous unknown name

No scoring reasons supplied.

Related Mech records

This family joined the worklist after the frozen cross-Mech scan, so other Mechs were not searched for it. Missing links here are not evidence that other Mechs lack it; rows shown come from mentions of a former DUF/UPF name.

No cross-Mech examples are present in the supplied snapshot.

Curation history and reviews

No retained curation events supplied.

No retained review supplied.

Record source

data/families/PF03781.yaml
Family YAML in this build

Download this family (JSON)