All families / PF12130
PF12130: bMERB_dom
Bivalent Mical/EHBP Rab binding domain
- Curation status
- SEEDED
- Seed status
- EX DUF
- Characterization
- UNSCORED
Grounding
PF12130: Bivalent Mical/EHBP Rab binding domain
IPR022735: integrated entry for Bivalent Mical/EHBP Rab binding domain
Source description
A variety of different effector proteins interact specifically with GTP-bound Rab proteins and mediate their versatile roles in membrane trafficking, including budding of vesicles from a donor membrane, directed transport through the cell and finally tethering and fusion with a target membrane. The 'bivalent Mical/EHBP Rab binding' (bMERB) domain is a Rab effector domain that is present in proteins of the Mical and EHBP families, both known to act in endosomal trafficking. The bMERB domain displays a preference for Rab8 family proteins (Rab8, 10, 13 and 15) and at least some of the bMERB domains contain two separate binding sites for Rab-proteins, allowing Micals and EHBPs to bind two Rabs simultaneously. The strong similarity between the two binding sites within one bMRB domain strongly suggests an evolutionarily development via duplication of a common ancestor supersecondary structure element. The bMERB domain has a completely alpha-helical fold consisting of a central helix and N- and C-terminal helices folding back on this central helix [InterPro reference PUB00088663].
Evidence and references
- InterPro entry and its literature references. PUB identifiers are internal InterPro references, not PubMed identifiers. Unresolved citations link to the originating entry.
- Frozen description source: InterPro/Pfam API
Source descriptions and links are provenance, not independent functional validation.
Evidence counts: not scored.
Curated functional assertions
No curated functional assertions supplied.
Protein sequence examples
8 verified sequence examples. FASTA
| UniProt | Protein / organism | Sequence | Provenance |
|---|---|---|---|
| O94851 | [F-actin]-monooxygenase MICAL2 Homo sapiens | 1957 aa; version 2 UniProt 2026_03 | UNIPROT FETCH2 sibling references |
| Q69ZW3 | EH domain-binding protein 1 Mus musculus | 1231 aa; version 3 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| Q8N3D4 | EH domain-binding protein 1-like protein 1 Homo sapiens | 1523 aa; version 2 UniProt 2026_03 | UNIPROT FETCH2 sibling references |
| Q8NDI1 | EH domain-binding protein 1 Homo sapiens | 1231 aa; version 3 UniProt 2026_03 | UNIPROT FETCH2 sibling references |
| Q8R1Y2 | bMERB domain-containing protein 1 Mus musculus | 203 aa; version 1 UniProt 2026_03 | UNIPROT FETCH |
| Q8TDZ2 | [F-actin]-monooxygenase MICAL1 Homo sapiens | 1067 aa; version 2 UniProt 2026_03 | UNIPROT FETCH1 sibling references |
| Q96MC5 | bMERB domain-containing protein 1 Homo sapiens | 204 aa; version 1 UniProt 2026_03 | PTM REGISTRY MIRROR |
| Q99MS7 | EH domain-binding protein 1-like protein 1 Mus musculus | 1716 aa; version 1 UniProt 2026_03 | UNIPROT FETCH |
Pfam membership is not functional evidence. Whole-protein sequences do not establish domain boundaries. A coverage gap here is not absence from UniProt.
Sequences and metadata: UniProt Consortium, CC BY 4.0.
Domain matches
No residue-level domain match evidence is available in this build. Structure and AlphaFold counters do not supply coordinates or establish function.
Snapshot counters
- proteins
- 12,866
- matches
- 13,023
- proteomes
- 1,275
- taxa
- 4,099
- structures
- 14
- alphafold models
- 7,449
- domain architectures
- 118
Classification basis
pfam previous unknown name
No scoring reasons supplied.
Related Mech records
This family joined the worklist after the frozen cross-Mech scan, so other Mechs were not searched for it. Missing links here are not evidence that other Mechs lack it; rows shown come from mentions of a former DUF/UPF name.
No cross-Mech examples are present in the supplied snapshot.
Curation history and reviews
No retained curation events supplied.
No retained review supplied.
Record source
Status and evidence
- Seed status
- A name-based classification of frozen InterPro/Pfam metadata. UNKNOWN CANDIDATE means the metadata explicitly says the function is unknown. KNOWN HISTORICAL DUF means a DUF name matched without that wording; this heuristic is not proof of a known function and does not establish experimental characterization. EX DUF means Pfam renamed the family away from a DUF/UPF name, as recorded in Pfam's previous identifiers; a rename usually follows published work on some members but is naming history, not proof of function for every member. Some EX DUF families' current names or descriptions still say the function is unknown; their classification basis lists this, and scoring does not treat them as characterized. FALSE POSITIVE TEXT HIT means the naming rules did not match.
- Characterization
- A separate evidence-scoring result. UNSCORED means no score has been calculated; it is not evidence that the family lacks a known function. Missing scores are not negative evidence. PARTIALLY CHARACTERIZED has partial support; KNOWN HISTORICAL DUF can inherit the seed label or reflect a known-function signal. Neither label implies experimental validation.
- Evidence counts
- Source-specific signals, not unique proteins, publications, or confidence scores. Known counts Rhea reactions and experimentally supported GO molecular-function annotations. Partial counts functional/domain assignments. Context counts structural, environmental, orthology, interaction and neighborhood signals; context alone does not assign function. Repeated annotations may not be independent.
- Curation status
- Reported separately from seed classification and characterization. SNAPSHOT ONLY means no curated metadata was supplied for this build. SEEDED denotes a source-derived record, not an expert-reviewed functional claim.
- Missing counts
- Not available means the source did not supply that counter. A zero is a reported count. Not scored means evidence counts are unavailable.