distamycin

naturalproductmech:mibig-5fbc59612b MINTED SEEDED antiparasiticenzyme inhibitor

Grounding review: No 3-star ChEBI entry shares this structure, so the record keeps a content-hashed identifier.

What these status tags mean

Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.

Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.

Structure

Standard InChIKey	UPBAOYRENQEPJO-UHFFFAOYSA-N
SMILES	CN1C=C(C=C1C(=O)NC2=CN(C(=C2)C(=O)NC3=CN(C(=C3)C(=O)NCCC(=N)N)C)C)NC=O
Stereochemistry	fully defined
Cross-references	pubchem:3115

Filing pathway

Alkaloids — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is nrps, from MIBiG.

Producer organisms 1

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

Taxon	Basis	Cluster	Evidence
Streptomyces netropsis NCBITaxon:55404	BGC_CHARACTERIZED	mibig:BGC0001147	PMID:25415678

Occurrences 0

None cited.

Biosynthetic gene clusters 1

Accession	Host	Locus evidence	Genome
mibig:BGC0001147	Streptomyces netropsis NCBITaxon:55404	CLUSTER_DEMONSTRATED	genbank:KJ634466.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Mechanism & Biosynthesis Overview

Bioactivities 6

Entries preserve source qualifiers, targets and evidence. Repeated assay names or values are not independent replications; inspect source detail for differences. 1 identical imported entry was collapsed for display only.

Assay	Result	Target enzyme	Source detail
Primary qHTS for delayed death inhibitors of the malarial parasite plastid, 48 hour incubation	OTHER 3.3808 uM; ACTIVE	Not recorded	pubchem.aid:504832 assay Primary qHTS for delayed death inhibitors of the malarial parasite plastid, 48 hour incubation evidence reference pubchem.aid:504832 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 504832, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. call ACTIVE measurement type OTHER value 3.3808 units uM activity class ENZYME_INHIBITOR
S16 Schwann cell PMP22 intronic element firefly luciferase assay	OTHER 36.1254 uM; NOT_DETERMINED	Not recorded	pubchem.aid:624032 assay S16 Schwann cell PMP22 intronic element firefly luciferase assay evidence reference pubchem.aid:624032 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 624032, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. Target accession NP_058733 was left ungrounded because PubChem did not provide a UniProtKB accession. call NOT_DETERMINED measurement type OTHER value 36.1254 units uM
qHTS assay for small molecule disruptors of the mitochondrial membrane potential	OTHER 33.4915 uM; NOT_DETERMINED	Not recorded	pubchem.aid:720635 assay qHTS assay for small molecule disruptors of the mitochondrial membrane potential evidence reference pubchem.aid:720635 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 720635, deposited by 824; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. call NOT_DETERMINED measurement type OTHER value 33.4915 units uM
qHTS for Inhibitors of Polymerase Kappa	OTHER 0.7943 uM; ACTIVE	Not recorded	pubchem.aid:588579 assay qHTS for Inhibitors of Polymerase Kappa evidence reference pubchem.aid:588579 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 588579, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. Target accession NP_057302 was left ungrounded because PubChem did not provide a UniProtKB accession. call ACTIVE measurement type OTHER value 0.7943 units uM activity class ENZYME_INHIBITOR
qHTS for Inhibitors of human tyrosyl-DNA phosphodiesterase 1 (TDP1): qHTS in cells in presence of CPT	OTHER 0.0188 uM; INACTIVE	Not recorded	pubchem.aid:686979 assay qHTS for Inhibitors of human tyrosyl-DNA phosphodiesterase 1 (TDP1): qHTS in cells in presence of CPT evidence reference pubchem.aid:686979 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 686979, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. Target accession AAI07878 was left ungrounded because PubChem did not provide a UniProtKB accession. call INACTIVE measurement type OTHER value 0.0188 units uM
qHTS profiling for inhibitors of Plasmodium falciparum proliferation	ACTIVE	Not recorded	PMID:21817045 assay qHTS profiling for inhibitors of Plasmodium falciparum proliferation evidence reference PMID:21817045 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 504749, deposited by NCGC; assay type Other. call ACTIVE activity class ANTIPARASITIC

Explore across X-Mech

AntibioticMech (distamycin) ↗ MediaIngredientMech (naturalproductmech:mibig-5fbc59612b) ↗ TaxonMech: Streptomyces netropsis (NCBITaxon:55404) ↗ PathwayMech (mibig:BGC0001147) ↗

Provenance

Source concept	Source	Version
BGC0001147	MIBIG	4

This page is generated from data/natural_products/alkaloids/distamycin.yaml, which is generated from the committed inventories. Neither is edited by hand.