fervenulin

naturalproductmech:mibig-f2f849b16f MINTED SEEDED antibacterialcytotoxicenzyme inhibitor

Grounding review: No 3-star ChEBI entry shares this structure, so the record keeps a content-hashed identifier.

What these status tags mean

Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.

Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.

Structure

Standard InChIKey	RRTKVYSLIGQWCO-UHFFFAOYSA-N
SMILES	CN1C2=C(C(=O)N(C1=O)C)N=CN=N2
Stereochemistry	fully defined
Cross-references	pubchem:249646

Filing pathway

Alkaloids — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is other, from MIBiG.

Producer organisms 1

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

Taxon	Basis	Cluster	Evidence
Streptomyces hiroshimensis NCBITaxon:66424	SOURCE_ASSERTION	mibig:BGC0001972	PMID:30565634

Occurrences 3

Somebody found the compound in this organism and cited it. That is not a claim that the organism makes it, and nothing here promotes one to the other.

Taxon	Source	Reference
Streptomyces hiroshimensis NCBITaxon:66424	LOTUS	DOI:10.1007/S11274-010-0588-Z
Streptomyces hiroshimensis NCBITaxon:66424	LOTUS	DOI:10.1007/S11306-016-1025-6
Streptomyces hiroshimensis NCBITaxon:66424	LOTUS	DOI:10.1039/C8OB02847H

Biosynthetic gene clusters 1

Accession	Host	Locus evidence	Genome
mibig:BGC0001972	Streptomyces hiroshimensis NCBITaxon:66424	CLUSTER_UNSTATED	genbank:MK256951.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Mechanism & Biosynthesis Overview

Bioactivities 17

Entries preserve source qualifiers, targets and evidence. Repeated assay names or values are not independent replications; inspect source detail for differences.

Explore across X-Mech

AntibioticMech (fervenulin) ↗ MediaIngredientMech (naturalproductmech:mibig-f2f849b16f) ↗ TaxonMech: Streptomyces hiroshimensis (NCBITaxon:66424) ↗ PathwayMech (mibig:BGC0001972) ↗

Provenance

Source concept	Source	Version
BGC0001972	MIBIG	4

This page is generated from data/natural_products/alkaloids/fervenulin.yaml, which is generated from the committed inventories. Neither is edited by hand.

Assay	Result	Target enzyme	Source detail
A screen for compounds that inhibit replication of Vibrio cholerae chromosome II	ACTIVE	Not recorded	pubchem.aid:504770 assay A screen for compounds that inhibit replication of Vibrio cholerae chromosome II evidence reference pubchem.aid:504770 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 504770, deposited by ICCB-Longwood/NSRB Screening Facility, Harvard Medical School; assay type Other. Target accession AAF95916 was left ungrounded because PubChem did not provide a UniProtKB accession. call ACTIVE
A screen for compounds that inhibit the activity of LtaS in Staphylococcus aureus	ACTIVE	Not recorded	pubchem.aid:720641 assay A screen for compounds that inhibit the activity of LtaS in Staphylococcus aureus evidence reference pubchem.aid:720641 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 720641, deposited by ICCB-Longwood/NSRB Screening Facility, Harvard Medical School; assay type Other. Target accession NP_371243 was left ungrounded because PubChem did not provide a UniProtKB accession. call ACTIVE activity class ANTIBACTERIAL
A screen for compounds that inhibit viral RNA polymerase binding and polymerization activities	ACTIVE	Not recorded	PMID:21722674 assay A screen for compounds that inhibit viral RNA polymerase binding and polymerization activities evidence reference PMID:21722674 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 588519, deposited by ICCB-Longwood/NSRB Screening Facility, Harvard Medical School; assay type Other. Target accession 1RA7_A was left ungrounded because PubChem did not provide a UniProtKB accession. call ACTIVE activity class ENZYME_INHIBITOR
High-throughput screen for inhibitors of the GIV GBA-motif interaction with Galpha-i G protein subunits	ACTIVE	Not recorded	pubchem.aid:1224905 assay High-throughput screen for inhibitors of the GIV GBA-motif interaction with Galpha-i G protein subunits evidence reference pubchem.aid:1224905 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1224905, deposited by ICCB-Longwood/NSRB Screening Facility, Harvard Medical School; assay type Screening. Target accession BAE44387 was left ungrounded because PubChem did not provide a UniProtKB accession. call ACTIVE activity class ENZYME_INHIBITOR
High-throughput screen for inhibitors of the GIV GBA-motif interaction with Galpha-i G protein subunits	ACTIVE	UniProtKB:P08753	pubchem.aid:1224905 assay High-throughput screen for inhibitors of the GIV GBA-motif interaction with Galpha-i G protein subunits evidence reference pubchem.aid:1224905 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1224905, deposited by ICCB-Longwood/NSRB Screening Facility, Harvard Medical School; assay type Screening. call ACTIVE target enzyme UniProtKB:P08753 activity class ENZYME_INHIBITOR
High-throughput screen for inhibitors of the GIV GBA-motif interaction with Galpha-i G protein subunits, FP Hit Confirmation	ACTIVE	Not recorded	pubchem.aid:1259350 assay High-throughput screen for inhibitors of the GIV GBA-motif interaction with Galpha-i G protein subunits, FP Hit Confirmation evidence reference pubchem.aid:1259350 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1259350, deposited by ICCB-Longwood/NSRB Screening Facility, Harvard Medical School; assay type Other. call ACTIVE activity class ENZYME_INHIBITOR
NCI In Vivo Anticancer Drug Screen. Data for tumor model P388 Leukemia (intraperitoneal) in B6D2F1 (BDF1) mice	ACTIVE	Not recorded	pubchem.aid:328 assay NCI In Vivo Anticancer Drug Screen. Data for tumor model P388 Leukemia (intraperitoneal) in B6D2F1 (BDF1) mice evidence reference pubchem.aid:328 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 328, deposited by DTP/NCI; assay type Other. call ACTIVE activity class CYTOTOXIC
OTUD3 deubiquitinase inhibition: Primary qHTS	ACTIVE	Not recorded	pubchem.aid:1645858 assay OTUD3 deubiquitinase inhibition: Primary qHTS evidence reference pubchem.aid:1645858 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1645858, deposited by 24642; assay type Other. Target accession NP_056022 was left ungrounded because PubChem did not provide a UniProtKB accession. call ACTIVE
UCHL1 deubiquitinase inhibition: Dose-response Confirmation	ACTIVE	UniProtKB:P09936	pubchem.aid:1645867 assay UCHL1 deubiquitinase inhibition: Dose-response Confirmation evidence reference pubchem.aid:1645867 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1645867, deposited by 24642; assay type Confirmatory. call ACTIVE target enzyme UniProtKB:P09936
UCHL1 deubiquitinase inhibition: Primary qHTS	ACTIVE	UniProtKB:P09936	pubchem.aid:1645859 assay UCHL1 deubiquitinase inhibition: Primary qHTS evidence reference pubchem.aid:1645859 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1645859, deposited by 24642; assay type Other. call ACTIVE target enzyme UniProtKB:P09936
USP10 deubiquitinase inhibition: Dose-response Confirmation	ACTIVE	UniProtKB:Q14694	pubchem.aid:1645863 assay USP10 deubiquitinase inhibition: Dose-response Confirmation evidence reference pubchem.aid:1645863 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1645863, deposited by 24642; assay type Confirmatory. call ACTIVE target enzyme UniProtKB:Q14694
USP10 deubiquitinase inhibition: Primary qHTS	ACTIVE	UniProtKB:Q14694	pubchem.aid:1645856 assay USP10 deubiquitinase inhibition: Primary qHTS evidence reference pubchem.aid:1645856 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1645856, deposited by 24642; assay type Other. call ACTIVE target enzyme UniProtKB:Q14694
USP17 deubiquitinase inhibition: Primary qHTS	ACTIVE	UniProtKB:A8MUK1	pubchem.aid:1645855 assay USP17 deubiquitinase inhibition: Primary qHTS evidence reference pubchem.aid:1645855 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1645855, deposited by 24642; assay type Other. call ACTIVE target enzyme UniProtKB:A8MUK1
USP28 deubiquitinase inhibition: Primary qHTS	ACTIVE	UniProtKB:Q96RU2	pubchem.aid:1645857 assay USP28 deubiquitinase inhibition: Primary qHTS evidence reference pubchem.aid:1645857 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1645857, deposited by 24642; assay type Other. call ACTIVE target enzyme UniProtKB:Q96RU2
USP30 deubiquitinase inhibition: Primary qHTS	ACTIVE	UniProtKB:Q70CQ3	pubchem.aid:1645860 assay USP30 deubiquitinase inhibition: Primary qHTS evidence reference pubchem.aid:1645860 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1645860, deposited by 24642; assay type Other. call ACTIVE target enzyme UniProtKB:Q70CQ3
USP8 deubiquitinase inhibition: Dose-response Confirmation	ACTIVE	UniProtKB:P40818	pubchem.aid:1645862 assay USP8 deubiquitinase inhibition: Dose-response Confirmation evidence reference pubchem.aid:1645862 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1645862, deposited by 24642; assay type Confirmatory. call ACTIVE target enzyme UniProtKB:P40818
USP8 deubiquitinase inhibition: Primary qHTS	ACTIVE	UniProtKB:P40818	pubchem.aid:1645853 assay USP8 deubiquitinase inhibition: Primary qHTS evidence reference pubchem.aid:1645853 evidence type DATABASE_ASSERTION notes PubChem BioAssay AID 1645853, deposited by 24642; assay type Other. call ACTIVE target enzyme UniProtKB:P40818