indolmycin

naturalproductmech:mibig-3f4d7c9236 MINTED SEEDED

Grounding review: No 3-star ChEBI entry shares this structure, so the record keeps a content-hashed identifier.

What these status tags mean

Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.

Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.

Structure

Standard InChIKey	GNTVWGDQPXCYBV-QPUJVOFHSA-N
SMILES	[H][C@](C)(C1=CNC2=CC=CC=C12)[C@@]1([H])OC(NC)=NC1=O
Stereochemistry	fully defined
Cross-references	npatlas:NPA015391, pubchem:3082186

Filing pathway

Alkaloids — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is other, from MIBiG.

Producer organisms 1

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

Taxon	Basis	Cluster	Evidence
Streptomyces griseus subsp. griseus NCBITaxon:67263	BGC_CHARACTERIZED	mibig:BGC0001206	PMID:25730866

Occurrences 2

Somebody found the compound in this organism and cited it. That is not a claim that the organism makes it, and nothing here promotes one to the other.

Taxon	Source	Reference
Streptomyces albus NCBITaxon:1888	LOTUS	DOI:10.1021/JA01483A056
Streptomyces griseus NCBITaxon:1911	LOTUS	DOI:10.1080/00021369.1978.10863158

Biosynthetic gene clusters 1

Accession	Host	Locus evidence	Genome
mibig:BGC0001206	Streptomyces griseus subsp. griseus NCBITaxon:67263	CLUSTER_DEMONSTRATED	genbank:KM596502.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Biosynthesis curated

Written by a curator with claim-level evidence on each step; not derived from a source. This is what the rest of the record exists to support.

Step	Enzyme	Reaction	Substrate → product	Evidence
1	Ind7 N-demethylindolmycin N-methyltransferase UniProtKB:A0A0D4BSP3	RHEA:24726	ungrounded → naturalproductmech:mibig-3f4d7c9236	DOI:10.1073/pnas.1419964112

Step 1: Ind7 catalyzes the terminal SAM-dependent N-methylation of N-demethylindolmycin to indolmycin. The immediate precursor is named in the evidence-backed notes and graph because the neutral N-demethylindolmycin structure is not grounded in the local corpus.

Mechanism & Biosynthesis Overview

Causal graph — biosynthesis 3 nodes, 2 edges

Partial Streptomyces griseus subsp. griseus ATCC 12648 indolmycin biosynthesis graph for the Ind7-catalyzed terminal N-methylation.

Subject	Predicate	Object	Notes	Evidence
N-demethylindolmycin	is N-methylated by	Ind7 N-demethylindolmycin N-methyltransferase	Purified Ind7 transfers a SAM methyl group onto N-demethylindolmycin in the terminal indolmycin biosynthetic step.	DOI:10.1073/pnas.1419964112 https://mite.lisc.univie.ac.at/repository/MITE0000384 https://www.ncbi.nlm.nih.gov/nuccore/KM596502.1 https://mibig.secondarymetabolites.org/repository/BGC0001206.5/BGC0001206.gbk https://www.uniprot.org/uniprotkb/A0A0D4BSP3 reference DOI:10.1073/pnas.1419964112 evidence type PRIMARY_EXPERIMENT snippet responsible for the last N-methyltransfer reaction notes Du et al. deleted ind7 in Streptomyces griseus subsp. griseus ATCC 12648 and observed N-demethylindolmycin accumulation, then verified in vitro that Ind7 converts N-demethylindolmycin to indolmycin. reference https://mite.lisc.univie.ac.at/repository/MITE0000384 evidence type DATABASE_ASSERTION snippet producing mature indolmycin retrieved on 2026-10-01 notes MITE0000384 annotates Streptomyces griseus Ind7/A0A0D4BSP3 as a SAM-dependent N-methyltransferase, links GenPept AJT38688.1 and MIBiG BGC0001206, and records N-methylation of N-demethylindolmycin to indolmycin as its example reaction. reference https://www.ncbi.nlm.nih.gov/nuccore/KM596502.1 evidence type DATABASE_ASSERTION snippet protein_id="AJT38688.1" retrieved on 2026-10-01 notes GenBank KM596502.1 is the Streptomyces griseus subsp. griseus ATCC 12648 indolmycin cluster sequence and maps ind7 to AJT38688.1. reference https://mibig.secondarymetabolites.org/repository/BGC0001206.5/BGC0001206.gbk evidence type DATABASE_ASSERTION snippet protein_id="AJT38688.1" retrieved on 2026-10-01 notes MIBiG BGC0001206.5 maps the ind7 CDS to GenPept AJT38688.1 in the indolmycin cluster. reference https://www.uniprot.org/uniprotkb/A0A0D4BSP3 evidence type DATABASE_ASSERTION snippet GN=ind7 retrieved on 2026-10-01 notes Current reviewed UniProtKB:A0A0D4BSP3 resolves as the 237 aa Streptomyces griseus Ind7 N-demethylindolmycin N-methyltransferase and cross-references EMBL KM596502 protein AJT38688.1.
Ind7 N-demethylindolmycin N-methyltransferase	forms	indolmycin	The native ind7 knockout accumulates N-demethylindolmycin and cannot produce mature indolmycin.	DOI:10.1073/pnas.1419964112 https://mite.lisc.univie.ac.at/repository/MITE0000384 https://www.ncbi.nlm.nih.gov/nuccore/KM596502.1 https://mibig.secondarymetabolites.org/repository/BGC0001206.5/BGC0001206.gbk https://www.uniprot.org/uniprotkb/A0A0D4BSP3 reference DOI:10.1073/pnas.1419964112 evidence type PRIMARY_EXPERIMENT snippet responsible for the last N-methyltransfer reaction notes Du et al. deleted ind7 in Streptomyces griseus subsp. griseus ATCC 12648 and observed N-demethylindolmycin accumulation, then verified in vitro that Ind7 converts N-demethylindolmycin to indolmycin. reference https://mite.lisc.univie.ac.at/repository/MITE0000384 evidence type DATABASE_ASSERTION snippet producing mature indolmycin retrieved on 2026-10-01 notes MITE0000384 annotates Streptomyces griseus Ind7/A0A0D4BSP3 as a SAM-dependent N-methyltransferase, links GenPept AJT38688.1 and MIBiG BGC0001206, and records N-methylation of N-demethylindolmycin to indolmycin as its example reaction. reference https://www.ncbi.nlm.nih.gov/nuccore/KM596502.1 evidence type DATABASE_ASSERTION snippet protein_id="AJT38688.1" retrieved on 2026-10-01 notes GenBank KM596502.1 is the Streptomyces griseus subsp. griseus ATCC 12648 indolmycin cluster sequence and maps ind7 to AJT38688.1. reference https://mibig.secondarymetabolites.org/repository/BGC0001206.5/BGC0001206.gbk evidence type DATABASE_ASSERTION snippet protein_id="AJT38688.1" retrieved on 2026-10-01 notes MIBiG BGC0001206.5 maps the ind7 CDS to GenPept AJT38688.1 in the indolmycin cluster. reference https://www.uniprot.org/uniprotkb/A0A0D4BSP3 evidence type DATABASE_ASSERTION snippet GN=ind7 retrieved on 2026-10-01 notes Current reviewed UniProtKB:A0A0D4BSP3 resolves as the 237 aa Streptomyces griseus Ind7 N-demethylindolmycin N-methyltransferase and cross-references EMBL KM596502 protein AJT38688.1.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 8 nodes, 7 edges

Native ATCC 12648 Ind1 and Ind2 references support a database-annotated precursor branch. C-methylation and NADH-dependent reduction are separate processes; the downstream connection to indolmycin is multistep and inferred. The primary study was not newly inspected in full, and ring assembly is not represented by these edges.

Subject	Predicate	Object	Notes	Evidence
Ind1 native C-methyltransferase	is annotated to catalyze	Annotated indolepyruvate C-methylation	Database-supported biosynthetic role, not a newly inspected primary experiment. No exact recombinant construct, kinetics, knockout phenotype or physiological sufficiency is asserted.	https://www.uniprot.org/uniprotkb/A0A0D4BS77/entry https://www.ncbi.nlm.nih.gov/nuccore/KM596502.1 https://www.ncbi.nlm.nih.gov/protein/AJT38682.1 reference https://www.uniprot.org/uniprotkb/A0A0D4BS77/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed entry version 27, sequence version 1. FUNCTION assigns SAM-dependent side-chain C-methylation of indolepyruvate in indolmycin biosynthesis. snippet to yield 3-methylindolepyruvate reference https://www.ncbi.nlm.nih.gov/nuccore/KM596502.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Native indolmycin cluster maps ind1 to AJT38682.1, KM596502.1:2494..3483, 329 aa. CDS-aware genetic-code-11 translation agrees with the deposited CDS, independently fetched GenPept and direct UniProt sequences. The native source is Streptomyces griseus subsp. griseus ATCC 12648, taxon 67263. reference https://www.ncbi.nlm.nih.gov/protein/AJT38682.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Versioned native ind1 protein. UniProt uses species taxon 1911, retains the ATCC 12648 nucleotide-reference attribution, and cross-references KM596502/AJT38682.1. This is native sequence identity, not independent verification of a recombinant assay construct.
Indolepyruvate (database substrate)	is the annotated substrate of	Annotated indolepyruvate C-methylation	The database-named intermediate remains ungrounded: no exact chemical identifier, protonation, tautomer or stereochemical mapping is adopted.	https://www.uniprot.org/uniprotkb/A0A0D4BS77/entry reference https://www.uniprot.org/uniprotkb/A0A0D4BS77/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed entry version 27, sequence version 1. FUNCTION names (indol-3-yl)pyruvate as the methyl acceptor; S-adenosyl-L-methionine supplies the methyl group.
Annotated indolepyruvate C-methylation	yields the annotated product	Methylindolepyruvate (database intermediate)	The database-named intermediate remains ungrounded: no exact chemical identifier, protonation, tautomer or stereochemical mapping is adopted.	https://www.uniprot.org/uniprotkb/A0A0D4BS77/entry reference https://www.uniprot.org/uniprotkb/A0A0D4BS77/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed entry version 27, sequence version 1. FUNCTION names 3-methylindolepyruvate as the product. The Ind1 and Ind2 catalytic annotations reference the same methylated substrate/product entity; the graph uses a shared ungrounded name, not an independently audited exact chemical CURIE.
Ind2 native reductase	is annotated to catalyze	Annotated NADH-dependent reduction	Database-supported biosynthetic role, not a newly inspected primary experiment. No exact recombinant construct, kinetics, knockout phenotype or physiological sufficiency is asserted. The by-similarity active-site annotation is not experimental mutagenesis evidence.	https://www.uniprot.org/uniprotkb/A0A0D4BSN8/entry https://www.ncbi.nlm.nih.gov/nuccore/KM596502.1 https://www.ncbi.nlm.nih.gov/protein/AJT38683.1 reference https://www.uniprot.org/uniprotkb/A0A0D4BSN8/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed entry version 25, sequence version 1. FUNCTION assigns NADH-dependent reduction of beta-methylindolepyruvate in indolmycin biosynthesis. snippet Catalyzes the NADH-dependent reduction of beta-methylindolepyruvate to yield indolmycenate reference https://www.ncbi.nlm.nih.gov/nuccore/KM596502.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Native indolmycin cluster maps ind2 to AJT38683.1, KM596502.1:3480..5192, 570 aa. CDS-aware genetic-code-11 translation agrees with the deposited CDS, independently fetched GenPept and direct UniProt sequences. The native source is Streptomyces griseus subsp. griseus ATCC 12648, taxon 67263. reference https://www.ncbi.nlm.nih.gov/protein/AJT38683.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Versioned native ind2 protein. UniProt uses species taxon 1911, retains the ATCC 12648 nucleotide-reference attribution, and cross-references KM596502/AJT38683.1. This is native sequence identity, not independent verification of a recombinant assay construct.
Methylindolepyruvate (database intermediate)	is the annotated substrate of	Annotated NADH-dependent reduction	The database-named intermediate remains ungrounded: no exact chemical identifier, protonation, tautomer or stereochemical mapping is adopted.	https://www.uniprot.org/uniprotkb/A0A0D4BSN8/entry reference https://www.uniprot.org/uniprotkb/A0A0D4BSN8/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed entry version 25, sequence version 1. FUNCTION names beta-methylindolepyruvate as the substrate. The reduction direction follows FUNCTION; the displayed Rhea equation is written in the opposite oxidation direction and is not copied as a biosynthetic direction.
Annotated NADH-dependent reduction	yields the annotated product	Indolmycenate (database product)	The database-named intermediate remains ungrounded: no exact chemical identifier, protonation, tautomer or stereochemical mapping is adopted.	https://www.uniprot.org/uniprotkb/A0A0D4BSN8/entry reference https://www.uniprot.org/uniprotkb/A0A0D4BSN8/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed entry version 25, sequence version 1. FUNCTION names indolmycenate as the reduction product. No exact Rhea reaction or intermediate chemical identifier is adopted.
Indolmycenate (database product)	is inferred to feed downstream biosynthesis of	indolmycin	Indolmycenate is not indolmycin. This edge does not assert ring closure, final methylation, sufficiency of Ind1/Ind2 or a fully reconstructed pathway.	https://www.uniprot.org/uniprotkb/A0A0D4BSN8/entry reference https://www.uniprot.org/uniprotkb/A0A0D4BSN8/entry evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes Reviewed entry version 25, sequence version 1. FUNCTION places the indolmycenate-forming reaction in indolmycin biosynthesis. The curator represents a multistep downstream relationship, not a direct Ind2-to-final-product reaction.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 13 nodes, 13 edges

Native sequence references are mapped by inference into the reported engineered expression context. Whole-cell production and subsequent non-enzymatic completion remain separate from individual enzyme activity. This graph does not change the structure's natural-product origin or add a natural E. coli producer claim.

Subject	Predicate	Object	Notes	Evidence
Ind0 native reference	is the native reference inferred for a product encoded in	E. coli I1234670P5 expression platform	This links a sequence reference to a named expression-platform gene. It does not establish the complete plasmid/fusion sequence, protein abundance, individual necessity, purified activity or substrate specificity. Methods, constructs and figure pixels remain uninspected; the native accession is not presented as the experimentally sequenced recombinant protein.	DOI:10.1039/d0sc05843b https://www.uniprot.org/uniprotkb/A0A0D4BSA1/entry https://www.ncbi.nlm.nih.gov/protein/AJT38681.1 reference DOI:10.1039/d0sc05843b evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes The reported platform includes ind0. Correspondence to this native sequence is a curator inference. reference https://www.uniprot.org/uniprotkb/A0A0D4BSA1/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Unreviewed entry version 33, sequence version 1; 321 aa, ind0, KM596502/AJT38681.1. The nucleotide reference names ATCC 12648. Species taxon 1911 on reviewed entries and native subspecies taxon 67263 are retained without conflation. reference https://www.ncbi.nlm.nih.gov/protein/AJT38681.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Native complement(KM596502.1:862..1827), ATCC 12648, taxon 67263. CDS-aware code-11 translation, deposited CDS, GenPept and UniProt sequences agree. The snippet is the deposited product label, not an experimental functional assignment. snippet Ind0
Ind1 native reference	is the native reference inferred for a product encoded in	E. coli I1234670P5 expression platform	This links a sequence reference to a named expression-platform gene. It does not establish the complete plasmid/fusion sequence, protein abundance, individual necessity, purified activity or substrate specificity. Methods, constructs and figure pixels remain uninspected; the native accession is not presented as the experimentally sequenced recombinant protein.	DOI:10.1039/d0sc05843b https://www.uniprot.org/uniprotkb/A0A0D4BS77/entry https://www.ncbi.nlm.nih.gov/protein/AJT38682.1 reference DOI:10.1039/d0sc05843b evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes The reported platform includes ind1. Correspondence to this native sequence is a curator inference. reference https://www.uniprot.org/uniprotkb/A0A0D4BS77/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed entry version 27, sequence version 1; 329 aa, ind1, KM596502/AJT38682.1. The nucleotide reference names ATCC 12648. Species taxon 1911 on reviewed entries and native subspecies taxon 67263 are retained without conflation. reference https://www.ncbi.nlm.nih.gov/protein/AJT38682.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Native KM596502.1:2494..3483, ATCC 12648, taxon 67263. CDS-aware code-11 translation, deposited CDS, GenPept and UniProt sequences agree. The snippet is the deposited product label, not an experimental functional assignment. snippet Ind1
Ind2 native reference	is the native reference inferred for a product encoded in	E. coli I1234670P5 expression platform	This links a sequence reference to a named expression-platform gene. It does not establish the complete plasmid/fusion sequence, protein abundance, individual necessity, purified activity or substrate specificity. Methods, constructs and figure pixels remain uninspected; the native accession is not presented as the experimentally sequenced recombinant protein.	DOI:10.1039/d0sc05843b https://www.uniprot.org/uniprotkb/A0A0D4BSN8/entry https://www.ncbi.nlm.nih.gov/protein/AJT38683.1 reference DOI:10.1039/d0sc05843b evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes The reported platform includes ind2. Correspondence to this native sequence is a curator inference. reference https://www.uniprot.org/uniprotkb/A0A0D4BSN8/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed entry version 25, sequence version 1; 570 aa, ind2, KM596502/AJT38683.1. The nucleotide reference names ATCC 12648. Species taxon 1911 on reviewed entries and native subspecies taxon 67263 are retained without conflation. reference https://www.ncbi.nlm.nih.gov/protein/AJT38683.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Native KM596502.1:3480..5192, ATCC 12648, taxon 67263. CDS-aware code-11 translation, deposited CDS, GenPept and UniProt sequences agree. The snippet is the deposited product label, not an experimental functional assignment. snippet Ind2
Ind3 native reference	is the native reference inferred for a product encoded in	E. coli I1234670P5 expression platform	This links a sequence reference to a named expression-platform gene. It does not establish the complete plasmid/fusion sequence, protein abundance, individual necessity, purified activity or substrate specificity. Methods, constructs and figure pixels remain uninspected; the native accession is not presented as the experimentally sequenced recombinant protein.	DOI:10.1039/d0sc05843b https://www.uniprot.org/uniprotkb/A0A0D4BST1/entry https://www.ncbi.nlm.nih.gov/protein/AJT38684.1 reference DOI:10.1039/d0sc05843b evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes The reported platform includes ind3. Correspondence to this native sequence is a curator inference. reference https://www.uniprot.org/uniprotkb/A0A0D4BST1/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Unreviewed entry version 25, sequence version 1; 452 aa, ind3, KM596502/AJT38684.1. The nucleotide reference names ATCC 12648. Species taxon 1911 on reviewed entries and native subspecies taxon 67263 are retained without conflation. reference https://www.ncbi.nlm.nih.gov/protein/AJT38684.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Native KM596502.1:5189..6547, ATCC 12648, taxon 67263. CDS-aware code-11 translation, deposited CDS, GenPept and UniProt sequences agree. The snippet is the deposited product label, not an experimental functional assignment. snippet Ind3
Ind4 native reference	is the native reference inferred for a product encoded in	E. coli I1234670P5 expression platform	This links a sequence reference to a named expression-platform gene. It does not establish the complete plasmid/fusion sequence, protein abundance, individual necessity, purified activity or substrate specificity. Methods, constructs and figure pixels remain uninspected; the native accession is not presented as the experimentally sequenced recombinant protein.	DOI:10.1039/d0sc05843b https://www.uniprot.org/uniprotkb/A0A0D4BS17/entry https://www.ncbi.nlm.nih.gov/protein/AJT38685.1 reference DOI:10.1039/d0sc05843b evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes The reported platform includes ind4. Correspondence to this native sequence is a curator inference. reference https://www.uniprot.org/uniprotkb/A0A0D4BS17/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Unreviewed entry version 32, sequence version 1; 383 aa, ind4, KM596502/AJT38685.1. The nucleotide reference names ATCC 12648. Species taxon 1911 on reviewed entries and native subspecies taxon 67263 are retained without conflation. reference https://www.ncbi.nlm.nih.gov/protein/AJT38685.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Native KM596502.1:6604..7755, ATCC 12648, taxon 67263. CDS-aware code-11 translation, deposited CDS, GenPept and UniProt sequences agree. The snippet is the deposited product label, not an experimental functional assignment. snippet Ind4
Ind6 native reference	is the native reference inferred for a product encoded in	E. coli I1234670P5 expression platform	This links a sequence reference to a named expression-platform gene. It does not establish the complete plasmid/fusion sequence, protein abundance, individual necessity, purified activity or substrate specificity. Methods, constructs and figure pixels remain uninspected; the native accession is not presented as the experimentally sequenced recombinant protein.	DOI:10.1039/d0sc05843b https://www.uniprot.org/uniprotkb/A0A0D4BS80/entry https://www.ncbi.nlm.nih.gov/protein/AJT38687.1 reference DOI:10.1039/d0sc05843b evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes The reported platform includes ind6. Correspondence to this native sequence is a curator inference. reference https://www.uniprot.org/uniprotkb/A0A0D4BS80/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Unreviewed entry version 14, sequence version 1; 229 aa, ind6, KM596502/AJT38687.1. The nucleotide reference names ATCC 12648. Species taxon 1911 on reviewed entries and native subspecies taxon 67263 are retained without conflation. reference https://www.ncbi.nlm.nih.gov/protein/AJT38687.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Native KM596502.1:8698..9387, ATCC 12648, taxon 67263. CDS-aware code-11 translation, deposited CDS, GenPept and UniProt sequences agree. The snippet is the deposited product label, not an experimental functional assignment. snippet Ind6
Ind7 native reference	is the native reference inferred for a product encoded in	E. coli I1234670P5 expression platform	This links a sequence reference to a named expression-platform gene. It does not establish the complete plasmid/fusion sequence, protein abundance, individual necessity, purified activity or substrate specificity. Methods, constructs and figure pixels remain uninspected; the native accession is not presented as the experimentally sequenced recombinant protein.	DOI:10.1039/d0sc05843b https://www.uniprot.org/uniprotkb/A0A0D4BSP3/entry https://www.ncbi.nlm.nih.gov/protein/AJT38688.1 reference DOI:10.1039/d0sc05843b evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes The reported platform includes ind7. Correspondence to this native sequence is a curator inference. reference https://www.uniprot.org/uniprotkb/A0A0D4BSP3/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed entry version 28, sequence version 1; 237 aa, ind7, KM596502/AJT38688.1. The nucleotide reference names ATCC 12648. Species taxon 1911 on reviewed entries and native subspecies taxon 67263 are retained without conflation. reference https://www.ncbi.nlm.nih.gov/protein/AJT38688.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Native KM596502.1:9353..10066, ATCC 12648, taxon 67263. CDS-aware code-11 translation, deposited CDS, GenPept and UniProt sequences agree. The snippet is the deposited product label, not an experimental functional assignment. snippet Ind7
Pel5 (Paenibacillus elgii B69, paper protein)	was represented by its gene in	E. coli I1234670P5 expression platform	Paper-named protein only; exact accession and construct are unresolved. Do not substitute Streptomyces Ind5, Bsp5, or the unrelated pectate lyases returned by the bounded UniProt gene-name query. Gene inclusion is not a protein-abundance measurement.	DOI:10.1039/d0sc05843b reference DOI:10.1039/d0sc05843b evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-11 notes The platform uses P. elgii B69 pel5 in place of native ind5. snippet from Paenibacillus elgii B69
E. coli I1234670P5 expression platform	was reported to produce a small amount of	indolmycin	Whole-cell result, not individual enzyme activity or sufficiency of the cloned genes alone. Endogenous host functions remain present. No precise yield, detection limit, E. coli substrain, culture protocol or independent spectrum/standard/stereochemical verification is imported.	DOI:10.1039/d0sc05843b reference DOI:10.1039/d0sc05843b evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-11 notes Main Results report indolmycin assigned by LC-MS in the co-expression culture. snippet cloned all necessary genes into four plasmids
E. coli I1234670P5 expression platform	was reported to accumulate	Indolmycenic acid (paper compound 5)	Keep the paper intermediate ungrounded; no exact protonation, stereochemical CURIE or equivalence to a particular database indolmycenate form is asserted. This outcome does not assign the contribution of each expressed protein or identify the endogenous E. coli aminotransferase.	DOI:10.1039/d0sc05843b reference DOI:10.1039/d0sc05843b evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-11 notes Main Results report accumulation of compound 5, named indolmycenic acid. snippet employed the full eight-gene construct
Indolmycenic acid (paper compound 5)	was the reported purified input to	Three-step chemical completion (non-enzymatic)	The synthetic follow-up is distinct from the small amount of indolmycin already detected in the engineered culture. No exact intermediate structure identifier or uninspected preparative details are adopted.	DOI:10.1039/d0sc05843b reference DOI:10.1039/d0sc05843b evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-11 notes Purified compound 5 was used in subsequent chemical conversion.
Three-step chemical completion (non-enzymatic)	was reported to furnish	indolmycin	Chemical completion, not a newly demonstrated Ind3/Ind6/Ind7 enzymatic sequence. Product naming follows the paper; supplementary spectra and stereochemical assignments were not independently inspected. Halogenated congeners and their bioactivities are not transferred.	DOI:10.1039/d0sc05843b reference DOI:10.1039/d0sc05843b evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-11 notes The three-step conversion esterifies compound 5, closes the oxazolinone ring, then methylates to indolmycin.
ind0/ind1/ind2-only system (reported)	was reported to yield less	Indolmycenic acid (paper compound 5)	Comparison is with I1234670P5, not a single-gene knockout. No numerical ratio, expression-matched control, specific causal assignment to a removed protein or claim that all eight genes are individually necessary is made.	DOI:10.1039/d0sc05843b reference DOI:10.1039/d0sc05843b evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-11 notes The ind0/ind1/ind2-only system yielded less compound 5 than the full platform; contributions from the removed genes were unresolved.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Explore across X-Mech

AntibioticMech (indolmycin) ↗ MediaIngredientMech (naturalproductmech:mibig-3f4d7c9236) ↗ TaxonMech: Streptomyces griseus subsp. griseus (NCBITaxon:67263) ↗ PathwayMech (mibig:BGC0001206) ↗

Open questions 2

curator-indolmycin-precursor-primary-and-chemical-scope — Which primary construct and chemical data resolve the Ind1/Ind2 assays, exact intermediates and downstream oxazolinone assembly?

curator-indolmycin-expression-constructs-and-homologs — Can full plasmid sequences, Pel5 identity and matched expression controls resolve the platform protein assignments?

Provenance

Source concept	Source	Version
BGC0001206	MIBIG	5

This page is generated from data/natural_products/alkaloids/indolmycin.yaml, which is generated from the committed inventories. Neither is edited by hand.