tenellin

naturalproductmech:mibig-34ab171b17 MINTED SEEDED

Grounding review: No 3-star ChEBI entry shares this structure, so the record keeps a content-hashed identifier.

What these status tags mean

Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.

Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.

Structure

Standard InChIKey	YELFBSBOFKWHSL-QDPFKYGGSA-N
SMILES	CCC(C)\C=C(/C)\C=C\C(=O)C1=C(O)C(=CN(O)C1=O)C1=CC=C(O)C=C1
Stereochemistry	undefined stereocentres — the InChIKey does not distinguish this compound from its stereoisomers
Cross-references	npatlas:NPA05743

Filing pathway

Alkaloids — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is nrps, pks, from MIBiG.

Producer organisms 1

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

Taxon	Basis	Cluster	Evidence
Beauveria bassiana NCBITaxon:176275	BGC_CHARACTERIZED	mibig:BGC0001049	PMID:21899331

Occurrences 1

Somebody found the compound in this organism and cited it. That is not a claim that the organism makes it, and nothing here promotes one to the other.

Taxon	Source	Reference
Beauveria bassiana NCBITaxon:176275	LOTUS	DOI:10.1039/C39740000281

Biosynthetic gene clusters 1

Accession	Host	Locus evidence	Genome
mibig:BGC0001049	Beauveria bassiana NCBITaxon:176275	CLUSTER_DEMONSTRATED	genbank:AM409327.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Mechanism & Biosynthesis Overview

Causal graph — biosynthesis 12 nodes, 13 edges

Partial CBS 110.25 reference view of tenellin assembly, oxidative ring expansion, N-hydroxylation and database-reported genetic effects. References are not verified experimental alleles; database annotations aggregate citations across strains and constructs. TenS/TenC collaboration is not a physical-complex claim. Named intermediates remain chemically ungrounded; variable source spellings, detailed precursor accounting and exact stereochemical assignments remain unresolved. Broader 2-pyridone impairment is separated from tenellin loss. No congener-specific glycosylation, siderophore mechanism, bioactivity target, host-infection phenotype or engineered domain-swap product is transferred to tenellin. CBS strain attribution is from the UniProt sequence citation; the nucleotide record lacks a strain qualifier.

Subject	Predicate	Object	Notes	Evidence
TenS (CBS 110.25 reference)	has_database_annotated_catalytic_role_in	TenS/TenC-dependent scaffold assembly	Selected CBS 110.25 reference, not a verified experimental allele.	https://www.uniprot.org/uniprotkb/A0JJU1/entry https://www.ncbi.nlm.nih.gov/protein/CAL69597.1 reference https://www.uniprot.org/uniprotkb/A0JJU1/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB FUNCTION annotation, release 2026_03, entry 104, sequence 1. Scaffold assembly role attributed to PMID:18266306 and PMID:34903054. No substrate carbon count, domain-absence assertion or complete balanced reaction is adopted. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct. snippet TenS catalyzes the assembly of the polyketide-amino acid backbone reference https://www.ncbi.nlm.nih.gov/protein/CAL69597.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes Reference identity: tenS/tens, NCBITaxon:176275; coded_by join(AM409327.1:7941..9214, AM409327.1:9328..12552,AM409327.1:12672..20892). Coordinates, strands, codon start, complete start/stop and source cross-references checked. The complete spliced native CDS, deposited translation, separately retrieved GenPept and current UniProt sequence agree exactly (4239 aa). The CBS 110.25 attribution comes from the UniProt sequence citation PMID:17216664 / DOI:10.1002/cbic.200600398; AM409327.1 itself has no strain qualifier. Protein-existence annotation remains 1: Evidence at protein level. No experimental construct identity or functional experiment is newly established.
TenC (CBS 110.25 reference)	has_database_annotated_catalytic_role_in	TenS/TenC-dependent scaffold assembly	Selected CBS 110.25 reference, not a verified experimental allele.	https://www.uniprot.org/uniprotkb/A0JJU0/entry https://www.ncbi.nlm.nih.gov/protein/CAL69596.1 reference https://www.uniprot.org/uniprotkb/A0JJU0/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB FUNCTION annotation, release 2026_03, entry 58, sequence 1. Collaborating trans-enoyl reductase role attributed to PMID:18266306 and PMID:34903054. Functional collaboration does not establish a stable physical TenS-TenC complex. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct. snippet TenC collaborates with the hybrid PKS-NRPS synthetase tenS reference https://www.ncbi.nlm.nih.gov/protein/CAL69596.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes Reference identity: tenC/tens3, NCBITaxon:176275; coded_by complement(AM409327.1:6093..7259). Coordinates, strands, codon start, complete start/stop and source cross-references checked. The complete spliced native CDS, deposited translation, separately retrieved GenPept and current UniProt sequence agree exactly (388 aa). The CBS 110.25 attribution comes from the UniProt sequence citation PMID:17216664 / DOI:10.1002/cbic.200600398; AM409327.1 itself has no strain qualifier. Protein-existence annotation remains 2: Evidence at transcript level. No experimental construct identity or functional experiment is newly established.
TenA (CBS 110.25 reference)	has_database_annotated_catalytic_role_in	Pretenellin A oxidative ring expansion	Selected CBS 110.25 reference, not a verified experimental allele.	https://www.uniprot.org/uniprotkb/A0JJT8/entry https://www.ncbi.nlm.nih.gov/protein/CAL69594.1 reference https://www.uniprot.org/uniprotkb/A0JJT8/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB FUNCTION annotation, release 2026_03, entry 46, sequence 1. Oxidative ring expansion of pretenellin A to pretenellin B attributed to PMID:19067514 and PMID:34903054. The separate pyridovericin branch is not tenellin and is not imported. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct. snippet TenA catalyzes an oxidative ring expansion of pretenellin A reference https://www.ncbi.nlm.nih.gov/protein/CAL69594.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes Reference identity: tenA/tens1, NCBITaxon:176275; coded_by join(AM409327.1:1717..2301, AM409327.1:2366..2891,AM409327.1:2954..3369). Coordinates, strands, codon start, complete start/stop and source cross-references checked. The complete spliced native CDS, deposited translation, separately retrieved GenPept and current UniProt sequence agree exactly (508 aa). The CBS 110.25 attribution comes from the UniProt sequence citation PMID:17216664 / DOI:10.1002/cbic.200600398; AM409327.1 itself has no strain qualifier. Protein-existence annotation remains 1: Evidence at protein level. No experimental construct identity or functional experiment is newly established.
TenB (CBS 110.25 reference)	has_database_annotated_catalytic_role_in	Pretenellin B N-hydroxylation	Selected CBS 110.25 reference, not a verified experimental allele.	https://www.uniprot.org/uniprotkb/A0JJT9/entry https://www.ncbi.nlm.nih.gov/protein/CAL69595.1 reference https://www.uniprot.org/uniprotkb/A0JJT9/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB FUNCTION annotation, release 2026_03, entry 70, sequence 1. Selective N-hydroxylation attributed to PMID:19067514 and PMID:34903054. The shared FUNCTION paragraph uses tellinin; the DISRUPTION PHENOTYPE names tenellin and accumulation of pretenellin B. Product labels follow those contextual annotations, not a new exact intermediate structure assignment. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct. snippet TenB catalyzes the selective N-hydroxylation of the 2-pyridone nitrogen reference https://www.ncbi.nlm.nih.gov/protein/CAL69595.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes Reference identity: tenB/tens2, NCBITaxon:176275; coded_by complement(join(AM409327.1:3777..5159, AM409327.1:5217..5453)). Coordinates, strands, codon start, complete start/stop and source cross-references checked. The complete spliced native CDS, deposited translation, separately retrieved GenPept and current UniProt sequence agree exactly (539 aa). The CBS 110.25 attribution comes from the UniProt sequence citation PMID:17216664 / DOI:10.1002/cbic.200600398; AM409327.1 itself has no strain qualifier. Protein-existence annotation remains 1: Evidence at protein level. No experimental construct identity or functional experiment is newly established.
TenS/TenC-dependent scaffold assembly	has_named_product	Pretenellin A (source spellings vary)	Partial database-derived pathway view.	https://www.uniprot.org/uniprotkb/A0JJU1/entry reference https://www.uniprot.org/uniprotkb/A0JJU1/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB FUNCTION annotation, release 2026_03, entry 104, sequence 1. Scaffold assembly role attributed to PMID:18266306 and PMID:34903054. No substrate carbon count, domain-absence assertion or complete balanced reaction is adopted. Named-intermediate view only; cofactors, stereochemical outcomes and detailed carbon accounting are omitted. The FUNCTION paragraph spells the early intermediate pretellinin A in places; the TenA-specific sentence names pretenellin A. No exact intermediate chemical identifiers are assigned. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.
Pretenellin A (source spellings vary)	is_named_substrate_of	Pretenellin A oxidative ring expansion	Partial database-derived pathway view.	https://www.uniprot.org/uniprotkb/A0JJT8/entry reference https://www.uniprot.org/uniprotkb/A0JJT8/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB FUNCTION annotation, release 2026_03, entry 46, sequence 1. Oxidative ring expansion of pretenellin A to pretenellin B attributed to PMID:19067514 and PMID:34903054. The separate pyridovericin branch is not tenellin and is not imported. Named-intermediate view only; cofactors, stereochemical outcomes and detailed carbon accounting are omitted. The FUNCTION paragraph spells the early intermediate pretellinin A in places; the TenA-specific sentence names pretenellin A. No exact intermediate chemical identifiers are assigned. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.
Pretenellin A oxidative ring expansion	has_named_product	Pretenellin B	Partial database-derived pathway view.	https://www.uniprot.org/uniprotkb/A0JJT8/entry reference https://www.uniprot.org/uniprotkb/A0JJT8/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB FUNCTION annotation, release 2026_03, entry 46, sequence 1. Oxidative ring expansion of pretenellin A to pretenellin B attributed to PMID:19067514 and PMID:34903054. The separate pyridovericin branch is not tenellin and is not imported. Named-intermediate view only; cofactors, stereochemical outcomes and detailed carbon accounting are omitted. The FUNCTION paragraph spells the early intermediate pretellinin A in places; the TenA-specific sentence names pretenellin A. No exact intermediate chemical identifiers are assigned. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.
Pretenellin B	is_named_substrate_of	Pretenellin B N-hydroxylation	Partial database-derived pathway view.	https://www.uniprot.org/uniprotkb/A0JJT9/entry reference https://www.uniprot.org/uniprotkb/A0JJT9/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB FUNCTION annotation, release 2026_03, entry 70, sequence 1. Selective N-hydroxylation attributed to PMID:19067514 and PMID:34903054. The shared FUNCTION paragraph uses tellinin; the DISRUPTION PHENOTYPE names tenellin and accumulation of pretenellin B. Product labels follow those contextual annotations, not a new exact intermediate structure assignment. Named-intermediate view only; cofactors, stereochemical outcomes and detailed carbon accounting are omitted. The FUNCTION paragraph spells the early intermediate pretellinin A in places; the TenA-specific sentence names pretenellin A. No exact intermediate chemical identifiers are assigned. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.
Pretenellin B N-hydroxylation	has_named_product	Tenellin (one stereocenter unspecified)	Partial database-derived pathway view.	https://www.uniprot.org/uniprotkb/A0JJT9/entry reference https://www.uniprot.org/uniprotkb/A0JJT9/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB FUNCTION annotation, release 2026_03, entry 70, sequence 1. Selective N-hydroxylation attributed to PMID:19067514 and PMID:34903054. The shared FUNCTION paragraph uses tellinin; the DISRUPTION PHENOTYPE names tenellin and accumulation of pretenellin B. Product labels follow those contextual annotations, not a new exact intermediate structure assignment. Named-intermediate view only; cofactors, stereochemical outcomes and detailed carbon accounting are omitted. The FUNCTION paragraph spells the early intermediate pretellinin A in places; the TenA-specific sentence names pretenellin A. No exact intermediate chemical identifiers are assigned. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.
TenA (CBS 110.25 reference)	has_database_annotated_disruption_associated_loss_of	Database-reported tenellin production contexts	Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.	https://www.uniprot.org/uniprotkb/A0JJT8/entry reference https://www.uniprot.org/uniprotkb/A0JJT8/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB DISRUPTION PHENOTYPE annotation, release 2026_03, entry 46, sequence 1. The database reports failure to produce tenellin after disruption (PMID:19067514 and PMID:34903054). This is an annotation spanning experimental contexts, not newly established strain-specific essentiality. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.
TenB (CBS 110.25 reference)	has_database_annotated_disruption_associated_loss_of	Database-reported tenellin production contexts	Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.	https://www.uniprot.org/uniprotkb/A0JJT9/entry reference https://www.uniprot.org/uniprotkb/A0JJT9/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB DISRUPTION PHENOTYPE annotation, release 2026_03, entry 70, sequence 1. The database reports failure to produce tenellin after disruption (PMID:19067514 and PMID:34903054). This is an annotation spanning experimental contexts, not newly established strain-specific essentiality. Pretenellin B accumulation is attributed to PMID:19067514; the separate pyridovericin accumulation attributed to PMID:34903054 is not treated as tenellin. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.
TenS (CBS 110.25 reference)	has_database_annotated_disruption_associated_impairment_of	Tenellin-type 2-pyridone and intermediate production (broader phenotype)	The reported phenotype covers a broader compound class and intermediates; it is not relabelled as complete loss of this exact tenellin structure.	https://www.uniprot.org/uniprotkb/A0JJU1/entry reference https://www.uniprot.org/uniprotkb/A0JJU1/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB DISRUPTION PHENOTYPE annotation, release 2026_03, entry 104, sequence 1. Impairment of tenellin-type 2-pyridones and intermediates, attributed to PMID:17216664 and PMID:34903054; no exact tenellin-only essentiality claim. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.
Database-reported tenellin production contexts	has_named_product	Tenellin (one stereocenter unspecified)	Product context, not a separate catalytic step or production in every mutant.	https://www.uniprot.org/uniprotkb/A0JJT9/entry reference https://www.uniprot.org/uniprotkb/A0JJT9/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB DISRUPTION PHENOTYPE annotation, release 2026_03, entry 70, sequence 1. The phenotype annotation explicitly names tenellin. Stored SMILES, InChI and InChIKey agree with PubChem CID54704235 and pinned RDKit but leave one stereocenter unspecified. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 13 nodes, 17 edges

Partial ARSEF 2860 reference view of tenellin assembly, oxidative ring expansion, N-hydroxylation and database-reported genetic effects. References are not verified experimental alleles; database annotations aggregate citations across strains and constructs. TenS/TenC collaboration is not a physical-complex claim. Named intermediates remain chemically ungrounded; variable source spellings, detailed precursor accounting and exact stereochemical assignments remain unresolved. Broader 2-pyridone impairment is separated from tenellin loss. No congener-specific glycosylation, siderophore mechanism, bioactivity target, host-infection phenotype or engineered domain-swap product is transferred to tenellin. J4UJ10 sequence version 2 is 544 aa; deposited EJP63692.1 and RefSeq XP_008600655.1 are 490 aa. UniProt marks the deposited gene model erroneous. The corrected protein is not sequence-identical to those records. Its exact native splice/experimental sequence remains REVIEW_NEEDED. TenR regulation does not establish direct DNA binding; the TenS induction citation mismatch is not resolved.

Subject	Predicate	Object	Notes	Evidence
TenS (ARSEF 2860 reference)	has_database_annotated_catalytic_role_in	TenS/TenC-dependent scaffold assembly	Selected ARSEF 2860 reference, not a verified experimental allele.	https://www.uniprot.org/uniprotkb/J4KMC1/entry https://www.ncbi.nlm.nih.gov/protein/EJP63694.1 reference https://www.uniprot.org/uniprotkb/J4KMC1/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB FUNCTION annotation, release 2026_03, entry 80, sequence 1. Scaffold assembly role attributed to PMID:18266306 and PMID:34903054. No substrate carbon count, domain-absence assertion or complete balanced reaction is adopted. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct. snippet TenS catalyzes the assembly of the polyketide-amino acid backbone reference https://www.ncbi.nlm.nih.gov/protein/EJP63694.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes Reference identity: tenS/BBA_07338, NCBITaxon:655819; coded_by join(JH725173.1:168696..169972, JH725173.1:170096..173318,JH725173.1:173416..181623). Coordinates, strands, codon start, complete start/stop and source cross-references checked. The complete spliced native CDS, deposited translation, separately retrieved GenPept and current UniProt sequence agree exactly (4235 aa). The scaffold and GenPept source qualifiers explicitly name ARSEF 2860. Protein-existence annotation remains 1: Evidence at protein level. No experimental construct identity or functional experiment is newly established.
TenC (ARSEF 2860 reference)	has_database_annotated_catalytic_role_in	TenS/TenC-dependent scaffold assembly	Selected ARSEF 2860 reference, not a verified experimental allele.	https://www.uniprot.org/uniprotkb/J5JCC9/entry https://www.ncbi.nlm.nih.gov/protein/EJP63693.1 reference https://www.uniprot.org/uniprotkb/J5JCC9/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB FUNCTION annotation, release 2026_03, entry 44, sequence 1. Collaborating trans-enoyl reductase role attributed to PMID:18266306 and PMID:34903054. Functional collaboration does not establish a stable physical TenS-TenC complex. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct. snippet TenC collaborates with the hybrid PKS-NRPS synthetase tenS reference https://www.ncbi.nlm.nih.gov/protein/EJP63693.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes Reference identity: tenC/BBA_07337, NCBITaxon:655819; coded_by complement(JH725173.1:166856..168022). Coordinates, strands, codon start, complete start/stop and source cross-references checked. The complete spliced native CDS, deposited translation, separately retrieved GenPept and current UniProt sequence agree exactly (388 aa). The scaffold and GenPept source qualifiers explicitly name ARSEF 2860. Protein-existence annotation remains 1: Evidence at protein level. No experimental construct identity or functional experiment is newly established.
TenA (ARSEF 2860 reference)	has_database_annotated_catalytic_role_in	Pretenellin A oxidative ring expansion	Selected ARSEF 2860 reference, not a verified experimental allele.	https://www.uniprot.org/uniprotkb/J5JKC1/entry https://www.ncbi.nlm.nih.gov/protein/EJP63691.1 reference https://www.uniprot.org/uniprotkb/J5JKC1/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB FUNCTION annotation, release 2026_03, entry 40, sequence 1. Oxidative ring expansion of pretenellin A to pretenellin B attributed to PMID:19067514 and PMID:34903054. The separate pyridovericin branch is not tenellin and is not imported. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct. snippet TenA catalyzes an oxidative ring expansion of pretenellin A reference https://www.ncbi.nlm.nih.gov/protein/EJP63691.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes Reference identity: tenA/BBA_07335, NCBITaxon:655819; coded_by join(JH725173.1:162502..163086, JH725173.1:163154..164158). Coordinates, strands, codon start, complete start/stop and source cross-references checked. The complete spliced native CDS, deposited translation, separately retrieved GenPept and current UniProt sequence agree exactly (529 aa). The scaffold and GenPept source qualifiers explicitly name ARSEF 2860. Protein-existence annotation remains 1: Evidence at protein level. No experimental construct identity or functional experiment is newly established.
TenB (ARSEF 2860 reference, corrected model)	has_database_annotated_catalytic_role_in	Pretenellin B N-hydroxylation	J4UJ10 sequence version 2 is 544 aa; deposited EJP63692.1 and RefSeq XP_008600655.1 are 490 aa. UniProt marks the deposited gene model erroneous. The corrected protein is not sequence-identical to those records. Its exact native splice/experimental sequence remains REVIEW_NEEDED.	https://www.uniprot.org/uniprotkb/J4UJ10/entry https://www.ncbi.nlm.nih.gov/protein/EJP63692.1 reference https://www.uniprot.org/uniprotkb/J4UJ10/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB FUNCTION annotation, release 2026_03, entry 52, sequence 2. Selective N-hydroxylation attributed to PMID:19067514 and PMID:34903054. The shared FUNCTION paragraph uses tellinin; the DISRUPTION PHENOTYPE names tenellin and accumulation of pretenellin B. Product labels follow those contextual annotations, not a new exact intermediate structure assignment. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct. snippet TenB catalyzes the selective N-hydroxylation of the 2-pyridone nitrogen reference https://www.ncbi.nlm.nih.gov/protein/EJP63692.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes Reference identity: tenB/BBA_07336, NCBITaxon:655819; coded_by complement(join(JH725173.1:164565..165962, JH725173.1:166179..166253)). Coordinates, strands, codon start, complete start/stop and source cross-references checked. J4UJ10 sequence version 2 is 544 aa; deposited EJP63692.1 and RefSeq XP_008600655.1 are 490 aa. UniProt marks the deposited gene model erroneous. The corrected protein is not sequence-identical to those records. Its exact native splice/experimental sequence remains REVIEW_NEEDED. The deposited 490-aa model reproduces from its scaffold CDS. Bounded sequence-guided removal of 54 nt within the annotated 216-nt intron gives two models compatible with the curated 544-aa protein, with noncanonical donor dinucleotides. These are compatibility checks only, not validated exon boundaries or repaired annotations. The scaffold and GenPept source qualifiers explicitly name ARSEF 2860. Protein-existence annotation remains 1: Evidence at protein level. No experimental construct identity or functional experiment is newly established.
TenS/TenC-dependent scaffold assembly	has_named_product	Pretenellin A (source spellings vary)	Partial database-derived pathway view.	https://www.uniprot.org/uniprotkb/J4KMC1/entry reference https://www.uniprot.org/uniprotkb/J4KMC1/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB FUNCTION annotation, release 2026_03, entry 80, sequence 1. Scaffold assembly role attributed to PMID:18266306 and PMID:34903054. No substrate carbon count, domain-absence assertion or complete balanced reaction is adopted. Named-intermediate view only; cofactors, stereochemical outcomes and detailed carbon accounting are omitted. The FUNCTION paragraph spells the early intermediate pretellinin A in places; the TenA-specific sentence names pretenellin A. No exact intermediate chemical identifiers are assigned. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.
Pretenellin A (source spellings vary)	is_named_substrate_of	Pretenellin A oxidative ring expansion	Partial database-derived pathway view.	https://www.uniprot.org/uniprotkb/J5JKC1/entry reference https://www.uniprot.org/uniprotkb/J5JKC1/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB FUNCTION annotation, release 2026_03, entry 40, sequence 1. Oxidative ring expansion of pretenellin A to pretenellin B attributed to PMID:19067514 and PMID:34903054. The separate pyridovericin branch is not tenellin and is not imported. Named-intermediate view only; cofactors, stereochemical outcomes and detailed carbon accounting are omitted. The FUNCTION paragraph spells the early intermediate pretellinin A in places; the TenA-specific sentence names pretenellin A. No exact intermediate chemical identifiers are assigned. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.
Pretenellin A oxidative ring expansion	has_named_product	Pretenellin B	Partial database-derived pathway view.	https://www.uniprot.org/uniprotkb/J5JKC1/entry reference https://www.uniprot.org/uniprotkb/J5JKC1/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB FUNCTION annotation, release 2026_03, entry 40, sequence 1. Oxidative ring expansion of pretenellin A to pretenellin B attributed to PMID:19067514 and PMID:34903054. The separate pyridovericin branch is not tenellin and is not imported. Named-intermediate view only; cofactors, stereochemical outcomes and detailed carbon accounting are omitted. The FUNCTION paragraph spells the early intermediate pretellinin A in places; the TenA-specific sentence names pretenellin A. No exact intermediate chemical identifiers are assigned. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.
Pretenellin B	is_named_substrate_of	Pretenellin B N-hydroxylation	Partial database-derived pathway view. J4UJ10 sequence version 2 is 544 aa; deposited EJP63692.1 and RefSeq XP_008600655.1 are 490 aa. UniProt marks the deposited gene model erroneous. The corrected protein is not sequence-identical to those records. Its exact native splice/experimental sequence remains REVIEW_NEEDED.	https://www.uniprot.org/uniprotkb/J4UJ10/entry reference https://www.uniprot.org/uniprotkb/J4UJ10/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB FUNCTION annotation, release 2026_03, entry 52, sequence 2. Selective N-hydroxylation attributed to PMID:19067514 and PMID:34903054. The shared FUNCTION paragraph uses tellinin; the DISRUPTION PHENOTYPE names tenellin and accumulation of pretenellin B. Product labels follow those contextual annotations, not a new exact intermediate structure assignment. Named-intermediate view only; cofactors, stereochemical outcomes and detailed carbon accounting are omitted. The FUNCTION paragraph spells the early intermediate pretellinin A in places; the TenA-specific sentence names pretenellin A. No exact intermediate chemical identifiers are assigned. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.
Pretenellin B N-hydroxylation	has_named_product	Tenellin (one stereocenter unspecified)	Partial database-derived pathway view. J4UJ10 sequence version 2 is 544 aa; deposited EJP63692.1 and RefSeq XP_008600655.1 are 490 aa. UniProt marks the deposited gene model erroneous. The corrected protein is not sequence-identical to those records. Its exact native splice/experimental sequence remains REVIEW_NEEDED.	https://www.uniprot.org/uniprotkb/J4UJ10/entry reference https://www.uniprot.org/uniprotkb/J4UJ10/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB FUNCTION annotation, release 2026_03, entry 52, sequence 2. Selective N-hydroxylation attributed to PMID:19067514 and PMID:34903054. The shared FUNCTION paragraph uses tellinin; the DISRUPTION PHENOTYPE names tenellin and accumulation of pretenellin B. Product labels follow those contextual annotations, not a new exact intermediate structure assignment. Named-intermediate view only; cofactors, stereochemical outcomes and detailed carbon accounting are omitted. The FUNCTION paragraph spells the early intermediate pretellinin A in places; the TenA-specific sentence names pretenellin A. No exact intermediate chemical identifiers are assigned. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.
TenA (ARSEF 2860 reference)	has_database_annotated_disruption_associated_loss_of	Database-reported tenellin production contexts	Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.	https://www.uniprot.org/uniprotkb/J5JKC1/entry reference https://www.uniprot.org/uniprotkb/J5JKC1/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB DISRUPTION PHENOTYPE annotation, release 2026_03, entry 40, sequence 1. The database reports failure to produce tenellin after disruption (PMID:19067514 and PMID:34903054). This is an annotation spanning experimental contexts, not newly established strain-specific essentiality. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.
TenB (ARSEF 2860 reference, corrected model)	has_database_annotated_disruption_associated_loss_of	Database-reported tenellin production contexts	Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct. J4UJ10 sequence version 2 is 544 aa; deposited EJP63692.1 and RefSeq XP_008600655.1 are 490 aa. UniProt marks the deposited gene model erroneous. The corrected protein is not sequence-identical to those records. Its exact native splice/experimental sequence remains REVIEW_NEEDED.	https://www.uniprot.org/uniprotkb/J4UJ10/entry reference https://www.uniprot.org/uniprotkb/J4UJ10/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB DISRUPTION PHENOTYPE annotation, release 2026_03, entry 52, sequence 2. The database reports failure to produce tenellin after disruption (PMID:19067514 and PMID:34903054). This is an annotation spanning experimental contexts, not newly established strain-specific essentiality. Pretenellin B accumulation is attributed to PMID:19067514; the separate pyridovericin accumulation attributed to PMID:34903054 is not treated as tenellin. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.
TenS (ARSEF 2860 reference)	has_database_annotated_disruption_associated_impairment_of	Tenellin-type 2-pyridone and intermediate production (broader phenotype)	The reported phenotype covers a broader compound class and intermediates; it is not relabelled as complete loss of this exact tenellin structure.	https://www.uniprot.org/uniprotkb/J4KMC1/entry reference https://www.uniprot.org/uniprotkb/J4KMC1/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB DISRUPTION PHENOTYPE annotation, release 2026_03, entry 80, sequence 1. Impairment of tenellin-type 2-pyridones and intermediates, attributed to PMID:17216664 and PMID:34903054; no exact tenellin-only essentiality claim. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.
Database-reported tenellin production contexts	has_named_product	Tenellin (one stereocenter unspecified)	Product context, not a separate catalytic step or production in every mutant.	https://www.uniprot.org/uniprotkb/J4UJ10/entry reference https://www.uniprot.org/uniprotkb/J4UJ10/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB DISRUPTION PHENOTYPE annotation, release 2026_03, entry 52, sequence 2. The phenotype annotation explicitly names tenellin. Stored SMILES, InChI and InChIKey agree with PubChem CID54704235 and pinned RDKit but leave one stereocenter unspecified. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.
TenR (ARSEF 2860 reference)	has_database_annotated_positive_expression_effect_on	TenS (ARSEF 2860 reference)	Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.	https://www.uniprot.org/uniprotkb/J4VZG1/entry https://www.uniprot.org/uniprotkb/J4KMC1/entry https://www.ncbi.nlm.nih.gov/protein/EJP63690.1 reference https://www.uniprot.org/uniprotkb/J4VZG1/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB FUNCTION annotation, release 2026_03, entry 72, sequence 1. TenR is annotated to positively regulate pathway-gene expression (PMID:34903054). No direct promoter binding, physical complex, gene deletion essentiality or native assay construct is asserted. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct. snippet Transcription factor that positively regulates the expression of the genes reference https://www.uniprot.org/uniprotkb/J4KMC1/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB INDUCTION annotation, release 2026_03, entry 80, sequence 1. The target INDUCTION annotation names tenR. TenS INDUCTION cites PMID:17216664, whereas TenR FUNCTION cites PMID:34903054; this citation mismatch is retained as unresolved, not interpreted as the same experiment. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct. reference https://www.ncbi.nlm.nih.gov/protein/EJP63690.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes Reference identity: tenR/BBA_07334, NCBITaxon:655819; coded_by complement(JH725173.1:158525..161152). Coordinates, strands, codon start, complete start/stop and source cross-references checked. The complete spliced native CDS, deposited translation, separately retrieved GenPept and current UniProt sequence agree exactly (875 aa). The scaffold and GenPept source qualifiers explicitly name ARSEF 2860. Protein-existence annotation remains 3: Inferred from homology. No experimental construct identity or functional experiment is newly established.
TenR (ARSEF 2860 reference)	has_database_annotated_positive_expression_effect_on	TenC (ARSEF 2860 reference)	Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.	https://www.uniprot.org/uniprotkb/J4VZG1/entry https://www.uniprot.org/uniprotkb/J5JCC9/entry reference https://www.uniprot.org/uniprotkb/J4VZG1/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB FUNCTION annotation, release 2026_03, entry 72, sequence 1. TenR is annotated to positively regulate pathway-gene expression (PMID:34903054). No direct promoter binding, physical complex, gene deletion essentiality or native assay construct is asserted. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct. reference https://www.uniprot.org/uniprotkb/J5JCC9/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB INDUCTION annotation, release 2026_03, entry 44, sequence 1. The target INDUCTION annotation names tenR and cites PMID:34903054. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.
TenR (ARSEF 2860 reference)	has_database_annotated_positive_expression_effect_on	TenA (ARSEF 2860 reference)	Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.	https://www.uniprot.org/uniprotkb/J4VZG1/entry https://www.uniprot.org/uniprotkb/J5JKC1/entry reference https://www.uniprot.org/uniprotkb/J4VZG1/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB FUNCTION annotation, release 2026_03, entry 72, sequence 1. TenR is annotated to positively regulate pathway-gene expression (PMID:34903054). No direct promoter binding, physical complex, gene deletion essentiality or native assay construct is asserted. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct. reference https://www.uniprot.org/uniprotkb/J5JKC1/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB INDUCTION annotation, release 2026_03, entry 40, sequence 1. The target INDUCTION annotation names tenR and cites PMID:34903054. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.
TenR (ARSEF 2860 reference)	has_database_annotated_positive_expression_effect_on	TenB (ARSEF 2860 reference, corrected model)	Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct. J4UJ10 sequence version 2 is 544 aa; deposited EJP63692.1 and RefSeq XP_008600655.1 are 490 aa. UniProt marks the deposited gene model erroneous. The corrected protein is not sequence-identical to those records. Its exact native splice/experimental sequence remains REVIEW_NEEDED.	https://www.uniprot.org/uniprotkb/J4VZG1/entry https://www.uniprot.org/uniprotkb/J4UJ10/entry reference https://www.uniprot.org/uniprotkb/J4VZG1/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB FUNCTION annotation, release 2026_03, entry 72, sequence 1. TenR is annotated to positively regulate pathway-gene expression (PMID:34903054). No direct promoter binding, physical complex, gene deletion essentiality or native assay construct is asserted. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct. reference https://www.uniprot.org/uniprotkb/J4UJ10/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB INDUCTION annotation, release 2026_03, entry 52, sequence 2. The target INDUCTION annotation names tenR and cites PMID:34903054. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 6 nodes, 5 edges

Partial database-derived genetic production-effect view, separate from direct tenellin assembly. Native ARSEF 2860 references SIDA, SIDDA/SIDD, SIDF and SIDI have disruption annotations explicitly naming impaired tenellin production. Their usual siderophore catalytic roles are not transferred into tenellin biosynthetic reactions. Neither complete production loss, direct catalysis, ornithine incorporation, a specific regulatory mechanism nor verified assayed alleles is asserted. SIDC/SIDL/SIDH lack an equally specific tenellin production-effect statement in the inspected annotations; they remain follow-up leads, not proven irrelevant. No source-owned producer or cluster grade changes.

Subject	Predicate	Object	Notes	Evidence
SIDA (ARSEF 2860 reference; genetic effect)	has_database_annotated_disruption_associated_impairment_of	Database-reported tenellin production in siderophore-gene disruption contexts	Reference protein, not a verified experimental allele. Genetic effect does not establish a catalytic step in tenellin assembly.	https://www.uniprot.org/uniprotkb/J4W6X9/entry https://www.ncbi.nlm.nih.gov/protein/EJP66050.1 reference https://www.uniprot.org/uniprotkb/J4W6X9/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB DISRUPTION PHENOTYPE annotation, release 2026_03, entry 53, sequence 1. The DISRUPTION PHENOTYPE annotation explicitly includes impaired tenellin production among multiple affected products (PMID:39109629, DOI:10.1021/acs.jafc.4c02957). This is a genetic production-effect claim, not complete loss, direct tenellin catalysis, an ornithine-derived tenellin precursor claim or an established indirect regulatory mechanism. SIDDA/SIDF/SIDI text says six products but lists seven; no product count is adopted. The current UniProt gene symbol SIDDA is retained beside the FUNCTION label SIDD. Primary methods were not inspected: the publisher returned HTTP403 and no alternate retrieval was attempted. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct. snippet rhodotorulic acid (RA) and tenellin reference https://www.ncbi.nlm.nih.gov/protein/EJP66050.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes Reference identity: SIDA/BBA_05021, NCBITaxon:655819; coded_by join(JH725161.1:424117..425081, JH725161.1:425142..425709,JH725161.1:425768..425845). Coordinates, strands, codon start, complete start/stop and source cross-references checked. The complete spliced native CDS, deposited translation, separately retrieved GenPept and current UniProt sequence agree exactly (536 aa). The scaffold and GenPept source qualifiers explicitly name ARSEF 2860. Protein-existence annotation remains 3: Inferred from homology. No experimental construct identity or functional experiment is newly established.
SIDDA / SIDD (ARSEF 2860 reference; genetic effect)	has_database_annotated_disruption_associated_impairment_of	Database-reported tenellin production in siderophore-gene disruption contexts	Reference protein, not a verified experimental allele. Genetic effect does not establish a catalytic step in tenellin assembly.	https://www.uniprot.org/uniprotkb/J4UJF5/entry https://www.ncbi.nlm.nih.gov/protein/EJP63992.1 reference https://www.uniprot.org/uniprotkb/J4UJF5/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB DISRUPTION PHENOTYPE annotation, release 2026_03, entry 66, sequence 1. The DISRUPTION PHENOTYPE annotation explicitly includes impaired tenellin production among multiple affected products (PMID:39109629, DOI:10.1021/acs.jafc.4c02957). This is a genetic production-effect claim, not complete loss, direct tenellin catalysis, an ornithine-derived tenellin precursor claim or an established indirect regulatory mechanism. SIDDA/SIDF/SIDI text says six products but lists seven; no product count is adopted. The current UniProt gene symbol SIDDA is retained beside the FUNCTION label SIDD. Primary methods were not inspected: the publisher returned HTTP403 and no alternate retrieval was attempted. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct. snippet rhodotorulic acid (RA) and tenellin reference https://www.ncbi.nlm.nih.gov/protein/EJP63992.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes Reference identity: SIDDA/BBA_06997, NCBITaxon:655819; coded_by JH725171.1:154330..159861. Coordinates, strands, codon start, complete start/stop and source cross-references checked. The complete spliced native CDS, deposited translation, separately retrieved GenPept and current UniProt sequence agree exactly (1843 aa). The scaffold and GenPept source qualifiers explicitly name ARSEF 2860. Protein-existence annotation remains 3: Inferred from homology. No experimental construct identity or functional experiment is newly established.
SIDF (ARSEF 2860 reference; genetic effect)	has_database_annotated_disruption_associated_impairment_of	Database-reported tenellin production in siderophore-gene disruption contexts	Reference protein, not a verified experimental allele. Genetic effect does not establish a catalytic step in tenellin assembly.	https://www.uniprot.org/uniprotkb/J4W0D1/entry https://www.ncbi.nlm.nih.gov/protein/EJP63990.1 reference https://www.uniprot.org/uniprotkb/J4W0D1/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB DISRUPTION PHENOTYPE annotation, release 2026_03, entry 36, sequence 1. The DISRUPTION PHENOTYPE annotation explicitly includes impaired tenellin production among multiple affected products (PMID:39109629, DOI:10.1021/acs.jafc.4c02957). This is a genetic production-effect claim, not complete loss, direct tenellin catalysis, an ornithine-derived tenellin precursor claim or an established indirect regulatory mechanism. SIDDA/SIDF/SIDI text says six products but lists seven; no product count is adopted. The current UniProt gene symbol SIDDA is retained beside the FUNCTION label SIDD. Primary methods were not inspected: the publisher returned HTTP403 and no alternate retrieval was attempted. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct. snippet rhodotorulic acid (RA) and tenellin reference https://www.ncbi.nlm.nih.gov/protein/EJP63990.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes Reference identity: SIDF/BBA_06995, NCBITaxon:655819; coded_by join(JH725171.1:148284..149288, JH725171.1:149368..149730). Coordinates, strands, codon start, complete start/stop and source cross-references checked. The complete spliced native CDS, deposited translation, separately retrieved GenPept and current UniProt sequence agree exactly (455 aa). The scaffold and GenPept source qualifiers explicitly name ARSEF 2860. Protein-existence annotation remains 3: Inferred from homology. No experimental construct identity or functional experiment is newly established.
SIDI (ARSEF 2860 reference; genetic effect)	has_database_annotated_disruption_associated_impairment_of	Database-reported tenellin production in siderophore-gene disruption contexts	Reference protein, not a verified experimental allele. Genetic effect does not establish a catalytic step in tenellin assembly.	https://www.uniprot.org/uniprotkb/J5JLF2/entry https://www.ncbi.nlm.nih.gov/protein/EJP63991.1 reference https://www.uniprot.org/uniprotkb/J5JLF2/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB DISRUPTION PHENOTYPE annotation, release 2026_03, entry 45, sequence 1. The DISRUPTION PHENOTYPE annotation explicitly includes impaired tenellin production among multiple affected products (PMID:39109629, DOI:10.1021/acs.jafc.4c02957). This is a genetic production-effect claim, not complete loss, direct tenellin catalysis, an ornithine-derived tenellin precursor claim or an established indirect regulatory mechanism. SIDDA/SIDF/SIDI text says six products but lists seven; no product count is adopted. The current UniProt gene symbol SIDDA is retained beside the FUNCTION label SIDD. Primary methods were not inspected: the publisher returned HTTP403 and no alternate retrieval was attempted. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct. snippet rhodotorulic acid (RA) and tenellin reference https://www.ncbi.nlm.nih.gov/protein/EJP63991.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes Reference identity: SIDI/BBA_06996, NCBITaxon:655819; coded_by complement(join(JH725171.1:150011..151067, JH725171.1:151145..151818)). Coordinates, strands, codon start, complete start/stop and source cross-references checked. The complete spliced native CDS, deposited translation, separately retrieved GenPept and current UniProt sequence agree exactly (576 aa). The scaffold and GenPept source qualifiers explicitly name ARSEF 2860. Protein-existence annotation remains 3: Inferred from homology. No experimental construct identity or functional experiment is newly established.
Database-reported tenellin production in siderophore-gene disruption contexts	has_named_product	Tenellin (one stereocenter unspecified)	The source explicitly names tenellin, but its complete experimental structure/assay context was not independently inspected.	https://www.uniprot.org/uniprotkb/J4W6X9/entry reference https://www.uniprot.org/uniprotkb/J4W6X9/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed UniProtKB DISRUPTION PHENOTYPE annotation, release 2026_03, entry 53, sequence 1. The DISRUPTION PHENOTYPE annotation explicitly includes impaired tenellin production among multiple affected products (PMID:39109629, DOI:10.1021/acs.jafc.4c02957). This is a genetic production-effect claim, not complete loss, direct tenellin catalysis, an ornithine-derived tenellin precursor claim or an established indirect regulatory mechanism. SIDDA/SIDF/SIDI text says six products but lists seven; no product count is adopted. The current UniProt gene symbol SIDDA is retained beside the FUNCTION label SIDD. Primary methods were not inspected: the publisher returned HTTP403 and no alternate retrieval was attempted. Database annotation, not independently inspected primary experiments or a verified assayed allele. No generalization of a deletion phenotype to every native strain or construct.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Explore across X-Mech

AntibioticMech (tenellin) ↗ MediaIngredientMech (naturalproductmech:mibig-34ab171b17) ↗ TaxonMech: Beauveria bassiana (NCBITaxon:176275) ↗ PathwayMech (mibig:BGC0001049) ↗

Open questions 1

curator-tenellin-reference-model-and-evidence-limits — Which experimental sequences resolve ARSEF TenB and the TenS induction citation, and what primary evidence establishes exact intermediates and product stereochemistry?

Provenance

Source concept	Source	Version
BGC0001049	MIBIG	4

This page is generated from data/natural_products/alkaloids/tenellin.yaml, which is generated from the committed inventories. Neither is edited by hand.