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puromycin

CHEBI:17939 EXACT SEEDED antibacterialcytotoxicenzyme inhibitortoxin

What these status tags mean

Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.

Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.

An aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that causes premature chain termination during translation taking place in the ribosome.

Structure

Standard InChIKeyRXWNCPJZOCPEPQ-NVWDDTSBSA-N
SMILES[H][C@](N)(CC1=CC=C(OC)C=C1)C(=O)N[C@@H]1[C@@H](CO)O[C@H]([C@@H]1O)N1C=NC2=C1N=CN=C2N(C)C
Stereochemistryfully defined
Cross-referencesnpatlas:NPA02054, pubchem:439530

Filing pathway

Carbohydrates — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is other, from MIBiG.

Producer organisms 1

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

TaxonBasisClusterEvidence
Streptomyces alboniger
NCBITaxon:132473		 SOURCE_ASSERTION mibig:BGC0000878 PMID:8576156

Occurrences 11

Somebody found the compound in this organism and cited it. That is not a claim that the organism makes it, and nothing here promotes one to the other.

TaxonSourceReference
Streptomyces
NCBITaxon:1883		 LOTUS DOI:10.1021/ACS.JNATPROD.7B00850
Streptomyces lividans
NCBITaxon:1916		 LOTUS DOI:10.1016/S0014-5793(97)01564-0
Acronychia pubescens
NCBITaxon:273126		 LOTUS DOI:10.1021/ACS.JNATPROD.8B00956
Streptomyces alboniger
NCBITaxon:132473		 LOTUS DOI:10.1016/S0014-5793(97)01564-0
Streptomyces alboniger
NCBITaxon:132473		 LOTUS DOI:10.1016/S0014-5793(98)01228-9
Streptomyces alboniger
NCBITaxon:132473		 LOTUS DOI:10.1021/BI00348A036
Streptomyces alboniger
NCBITaxon:132473		 LOTUS DOI:10.1074/JBC.271.3.1579
Streptomyces alboniger
NCBITaxon:132473		 LOTUS DOI:10.1111/J.1432-1033.1993.TB18454.X
Streptomyces alboniger
NCBITaxon:132473		 LOTUS DOI:10.1128/AAC.8.6.721
Streptomyces alboniger
NCBITaxon:132473		 LOTUS DOI:10.1128/JB.175.22.7474-7478.1993
Streptomyces alboniger
NCBITaxon:132473		 LOTUS DOI:10.7164/ANTIBIOTICS.38.1761

Biosynthetic gene clusters 1

AccessionHostLocus evidenceGenome
mibig:BGC0000878 Streptomyces alboniger
NCBITaxon:132473 		CLUSTER_UNSTATED genbank:X92429.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Mechanism & Biosynthesis Overview

PRODUCERS & OCCURRENCE 1 producer (0 causal) 11 cited occurrences BIOSYNTHETIC LOCUS 1 gene cluster 0 curated pathway steps NATURAL PRODUCT puromycin CHEBI:17939 Carbohydrates TARGETS & BIOACTIVITY 0 molecular targets 25 bioactivities · 0 graphs

Bioactivities 25

Entries preserve source qualifiers, targets and evidence. Repeated assay names or values are not independent replications; inspect source detail for differences.

AssayResultTarget enzymeSource detail
Constitutive androstane receptor (CAR) small molecule agonists, cell-based qHTS assay in HepG2 cellsOTHER 2.31 uM; INACTIVENot recorded
pubchem.aid:2202728
assay
Constitutive androstane receptor (CAR) small molecule agonists, cell-based qHTS assay in HepG2 cells
evidence
  • reference
    pubchem.aid:2202728
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2202728, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
INACTIVE
measurement type
OTHER
value
2.31
units
uM
Constitutive androstane receptor (CAR) small molecule agonists, cell-based qHTS assay in HepG2 cellsOTHER 2.31 uM; INACTIVENot recorded
pubchem.aid:2202728
assay
Constitutive androstane receptor (CAR) small molecule agonists, cell-based qHTS assay in HepG2 cells
evidence
  • reference
    pubchem.aid:2202728
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2202728, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. Target accession ADZ17385 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
INACTIVE
measurement type
OTHER
value
2.31
units
uM
Cytochrome P450 family 2 subfamily C member 9 (CYP2C9) small molecule antagonists: luciferase cell-based qHTS assayOTHER 23.9185 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1645842
assay
Cytochrome P450 family 2 subfamily C member 9 (CYP2C9) small molecule antagonists: luciferase cell-based qHTS assay
evidence
  • reference
    pubchem.aid:1645842
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1645842, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency. Target accession QLI62812 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
NOT_DETERMINED
measurement type
OTHER
value
23.9185
units
uM
Cytochrome P450 family 3 subfamily A member 4 (CYP3A4) small molecule antagonists: luciferase cell-based qHTS assayOTHER 9.5221 uM; ACTIVENot recorded
pubchem.aid:1645841
assay
Cytochrome P450 family 3 subfamily A member 4 (CYP3A4) small molecule antagonists: luciferase cell-based qHTS assay
evidence
  • reference
    pubchem.aid:1645841
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1645841, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. Target accession ABI96208 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
ACTIVE
measurement type
OTHER
value
9.5221
units
uM
Cytochrome P450 family 3 subfamily A member 7 (CYP3A7) small molecule antagonists: luciferase cell-based qHTS assayOTHER 23.9185 uM; ACTIVENot recorded
pubchem.aid:1963596
assay
Cytochrome P450 family 3 subfamily A member 7 (CYP3A7) small molecule antagonists: luciferase cell-based qHTS assay
evidence
  • reference
    pubchem.aid:1963596
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1963596, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
23.9185
units
uM
Cytochrome P450 family 3 subfamily A member 7 (CYP3A7) small molecule antagonists: luciferase cell-based qHTS assayOTHER 23.9185 uM; ACTIVENot recorded
pubchem.aid:1963596
assay
Cytochrome P450 family 3 subfamily A member 7 (CYP3A7) small molecule antagonists: luciferase cell-based qHTS assay
evidence
  • reference
    pubchem.aid:1963596
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1963596, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. Target accession NP_000756 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
ACTIVE
measurement type
OTHER
value
23.9185
units
uM
GF-AFC viability counterscreen for inhibitors of alpha-synuclein gene (SNCA) expressionOTHER 21.1923 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1671193
assay
GF-AFC viability counterscreen for inhibitors of alpha-synuclein gene (SNCA) expression
evidence
  • reference
    pubchem.aid:1671193
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1671193, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
21.1923
units
uM
P-glycoprotein substrates identified in KB-8-5-11 adenocarcinoma cell line, qHTS therapeutic library screenOTHER 29.0929 uM; NOT_DETERMINEDNot recorded
PMID:31515284
assay
P-glycoprotein substrates identified in KB-8-5-11 adenocarcinoma cell line, qHTS therapeutic library screen
evidence
  • reference
    PMID:31515284
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1346987, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
29.0929
units
uM
Primary fluorescence-based thiol-reactive (MSTI) qHTS assay for identification of artifact compounds.OTHER 13.3714 uM; INACTIVENot recorded
pubchem.aid:1845221
assay
Primary fluorescence-based thiol-reactive (MSTI) qHTS assay for identification of artifact compounds.
evidence
  • reference
    pubchem.aid:1845221
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1845221, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
INACTIVE
measurement type
OTHER
value
13.3714
units
uM
Primary qHTS for inhibitors of nuclear receptor binding SET domain protein 2 (NSD2) in an RCH-ACV wild type WT cells (2C)OTHER 0.2582 uM; ACTIVENot recorded
pubchem.aid:1645877
assay
Primary qHTS for inhibitors of nuclear receptor binding SET domain protein 2 (NSD2) in an RCH-ACV wild type WT cells (2C)
evidence
  • reference
    pubchem.aid:1645877
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1645877, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
0.2582
units
uM
activity class
ENZYME_INHIBITOR
Primary qHTS for inhibitors of nuclear receptor binding SET domain protein 2 (NSD2) in an RCH-ACV wild type WT cells (2C)OTHER 0.2582 uM; ACTIVEUniProtKB:O96028
pubchem.aid:1645877
assay
Primary qHTS for inhibitors of nuclear receptor binding SET domain protein 2 (NSD2) in an RCH-ACV wild type WT cells (2C)
evidence
  • reference
    pubchem.aid:1645877
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1645877, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
0.2582
units
uM
target enzyme
UniProtKB:O96028
activity class
ENZYME_INHIBITOR
Primary qHTS for inhibitors of nuclear receptor binding SET domain protein 2 (NSD2) in an isogenic RCH-ACV NSD2 p.E1099K mutant (9B)OTHER 0.4093 uM; ACTIVENot recorded
pubchem.aid:1645876
assay
Primary qHTS for inhibitors of nuclear receptor binding SET domain protein 2 (NSD2) in an isogenic RCH-ACV NSD2 p.E1099K mutant (9B)
evidence
  • reference
    pubchem.aid:1645876
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1645876, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
0.4093
units
uM
activity class
ENZYME_INHIBITOR
Primary qHTS for inhibitors of nuclear receptor binding SET domain protein 2 (NSD2) in an isogenic RCH-ACV NSD2 p.E1099K mutant (9B)OTHER 0.4093 uM; ACTIVEUniProtKB:O96028
pubchem.aid:1645876
assay
Primary qHTS for inhibitors of nuclear receptor binding SET domain protein 2 (NSD2) in an isogenic RCH-ACV NSD2 p.E1099K mutant (9B)
evidence
  • reference
    pubchem.aid:1645876
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1645876, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
0.4093
units
uM
target enzyme
UniProtKB:O96028
activity class
ENZYME_INHIBITOR
Primary qHTS for small molecule compounds that upregulate eGFP-tagged delta133p53alpha (in N-terminal) cells.OTHER 6.7456 uM; ACTIVENot recorded
pubchem.aid:2060322
assay
Primary qHTS for small molecule compounds that upregulate eGFP-tagged delta133p53alpha (in N-terminal) cells.
evidence
  • reference
    pubchem.aid:2060322
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2060322, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
6.7456
units
uM
Primary qHTS for small molecule compounds that upregulate eGFP-tagged delta133p53alpha (in N-terminal) cells.OTHER 6.7456 uM; ACTIVENot recorded
pubchem.aid:2060322
assay
Primary qHTS for small molecule compounds that upregulate eGFP-tagged delta133p53alpha (in N-terminal) cells.
evidence
  • reference
    pubchem.aid:2060322
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2060322, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency. Target accession NP_001119587 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
ACTIVE
measurement type
OTHER
value
6.7456
units
uM
Primary qHTS to identify anti-liver cancer compounds using libraries of approved drugs and bioactive compoundsOTHER 1.2589 uM; ACTIVENot recorded
pubchem.aid:2202548
assay
Primary qHTS to identify anti-liver cancer compounds using libraries of approved drugs and bioactive compounds
evidence
  • reference
    pubchem.aid:2202548
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2202548, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
1.2589
units
uM
activity class
CYTOTOXIC
Viability qHTS for spleen associated tyrosine kinase inhibitor (SYKi) drug resistant MV4-11 cellsOTHER 0.1836 uM; ACTIVENot recorded
pubchem.aid:1963824
assay
Viability qHTS for spleen associated tyrosine kinase inhibitor (SYKi) drug resistant MV4-11 cells
evidence
  • reference
    pubchem.aid:1963824
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1963824, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
0.1836
units
uM
Viability qHTS for spleen associated tyrosine kinase inhibitor (SYKi) drug resistant MV4-11 cellsOTHER 0.1836 uM; ACTIVEUniProtKB:P43405
pubchem.aid:1963824
assay
Viability qHTS for spleen associated tyrosine kinase inhibitor (SYKi) drug resistant MV4-11 cells
evidence
  • reference
    pubchem.aid:1963824
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1963824, deposited by NCGC; assay type Confirmatory. Source endpoint: Potency.
call
ACTIVE
measurement type
OTHER
value
0.1836
units
uM
target enzyme
UniProtKB:P43405
Viability qHTS for spleen associated tyrosine kinase inhibitor (SYKi) naive MV4-11 cellsOTHER 0.2311 uM; NOT_DETERMINEDNot recorded
pubchem.aid:1963823
assay
Viability qHTS for spleen associated tyrosine kinase inhibitor (SYKi) naive MV4-11 cells
evidence
  • reference
    pubchem.aid:1963823
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1963823, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
0.2311
units
uM
Viability qHTS for spleen associated tyrosine kinase inhibitor (SYKi) naive MV4-11 cellsOTHER 0.2311 uM; NOT_DETERMINEDUniProtKB:P43405
pubchem.aid:1963823
assay
Viability qHTS for spleen associated tyrosine kinase inhibitor (SYKi) naive MV4-11 cells
evidence
  • reference
    pubchem.aid:1963823
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1963823, deposited by NCGC; assay type Confirmatory. Source outcome: Inconclusive. Source endpoint: Potency.
call
NOT_DETERMINED
measurement type
OTHER
value
0.2311
units
uM
target enzyme
UniProtKB:P43405
Cytotoxic Profiling of Annotated Libraries Using Quantitative High-Throughput ScreeningACTIVENot recorded
PMID:31498718
assay
Cytotoxic Profiling of Annotated Libraries Using Quantitative High-Throughput Screening
evidence
  • reference
    PMID:31498718
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1296008, deposited by NCGC; assay type Confirmatory.
call
ACTIVE
activity class
CYTOTOXIC
GENE-TOX mutagenicity studiesACTIVENot recorded
pubchem.aid:1259408
assay
GENE-TOX mutagenicity studies
evidence
  • reference
    pubchem.aid:1259408
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1259408, deposited by 22071; assay type Other.
call
ACTIVE
activity class
TOXIN
High Throughput Screen to Identify Inhibitors of Mycobacterium tuberculosis H37RvACTIVENot recorded
pubchem.aid:1332
assay
High Throughput Screen to Identify Inhibitors of Mycobacterium tuberculosis H37Rv
evidence
  • reference
    pubchem.aid:1332
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 1332, deposited by Southern Research Institute; assay type Confirmatory.
call
ACTIVE
activity class
ENZYME_INHIBITOR
Luminescence Cell-Based Primary HTS to Identify Inhibitors of MITFACTIVENot recorded
pubchem.aid:2240
assay
Luminescence Cell-Based Primary HTS to Identify Inhibitors of MITF
evidence
  • reference
    pubchem.aid:2240
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2240, deposited by Broad Institute; assay type Screening. Target accession NP_937802 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
ACTIVE
activity class
ENZYME_INHIBITOR
Luminescence Cell-Based Primary HTS to Identify Inhibitors of STK33ACTIVENot recorded
pubchem.aid:2330
assay
Luminescence Cell-Based Primary HTS to Identify Inhibitors of STK33
evidence
  • reference
    pubchem.aid:2330
    evidence type
    DATABASE_ASSERTION
    notes
    PubChem BioAssay AID 2330, deposited by Broad Institute; assay type Screening. Target accession CAC29064 was left ungrounded because PubChem did not provide a UniProtKB accession.
call
ACTIVE
activity class
ENZYME_INHIBITOR
notes
12 further PubChem assay results are in data/raw/pubchem_bioassay.tsv but not written here.

Elsewhere in the fleet

Explore across X-Mech

AntibioticMech (puromycin) ↗ MediaIngredientMech (CHEBI:17939) ↗ TaxonMech: Streptomyces alboniger (NCBITaxon:132473) ↗ PathwayMech (mibig:BGC0000878) ↗

Provenance

Source conceptSourceVersion
BGC0000878MIBIG5
CHEBI:17939CHEBI3-star

This page is generated from data/natural_products/carbohydrates/puromycin.yaml, which is generated from the committed inventories. Neither is edited by hand.