NaturalProductMech

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A23187

naturalproductmech:mibig-18de581c96 MINTED SEEDED

Grounding review: No 3-star ChEBI entry shares this structure, so the record keeps a content-hashed identifier.
What these status tags mean

Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.

Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.

Structure

Standard InChIKeyHIYAVKIYRIFSCZ-CYEMHPAKSA-N
SMILESC[C@@H]1CC[C@]2([C@@H](C[C@H]([C@H](O2)[C@H](C)C(=O)C3=CC=CN3)C)C)O[C@@H]1CC4=NC5=C(O4)C=CC(=C5C(=O)O)NC
Stereochemistryfully defined
Cross-referencesnpatlas:NPA03386, npatlas:NPA003386

Filing pathway

Polyketides — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is pks, from MIBiG.

Producer organisms 1

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

TaxonBasisClusterEvidence
Streptomyces chartreusis NRRL 3882
NCBITaxon:1079985		 BGC_CHARACTERIZED mibig:BGC0000032 DOI:10.1021/ja00813a047

Occurrences 3

Somebody found the compound in this organism and cited it. That is not a claim that the organism makes it, and nothing here promotes one to the other.

TaxonSourceReference
Streptomyces
NCBITaxon:1883		 LOTUS DOI:10.1021/ACS.JNATPROD.6B00996
Streptomyces
NCBITaxon:1883		 LOTUS DOI:10.1021/JA00813A047
Streptomyces chartreusis
NCBITaxon:1969		 LOTUS DOI:10.1128/AAC.01130-10

Biosynthetic gene clusters 1

AccessionHostLocus evidenceGenome
mibig:BGC0000032 Streptomyces chartreusis NRRL 3882
NCBITaxon:1079985 		CLUSTER_DEMONSTRATED genbank:HM452329.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Mechanism & Biosynthesis Overview

PRODUCERS & OCCURRENCE 1 producer (1 causal) 3 cited occurrences BIOSYNTHETIC LOCUS 1 gene cluster 0 curated pathway steps NATURAL PRODUCT A23187 naturalproductmech:mibig-18de581c96 Polyketides TARGETS & BIOACTIVITY 0 molecular targets 0 bioactivities · 6 graphs

Causal graph — biosynthesis 7 nodes, 6 edges

Two sequence-verified native protein references, SchN1 and SchB2, are linked by explicit alias inferences to the 2011 calN1 and calB2 loci. Separate experimental edges describe internal neo-cassette replacement and loss of detectable calcimycin production in WQL2 and WQL4. These are whole-strain genetic effects, not direct enzyme reactions or proof against polar effects. No physical complex, CalN2 rescue transfer, CalA3 function or CalR3/CalT regulatory mechanism is asserted.

SubjectPredicateObjectNotesEvidence
SchN1 native reference is the native reference inferred for the product of calN1 (paper locus) The grounded node denotes the native reference protein. The paper locus remains separate; inferred alias correspondence is not an exact assayed-protein sequence claim.
DOI:10.1128/AAC.01130-10
UniProtKB:F8QZR3
https://www.ncbi.nlm.nih.gov/protein/AEH42483.1
https://www.ncbi.nlm.nih.gov/nuccore/HM452329.1
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Table 3 assigns calN1 a 379-aa product. The study deposits HM452329; its ordered gene position, compatible name, length and putative function map to SchN1. This alias mapping does not independently sequence the experimentally disrupted strain.
  • reference
    UniProtKB:F8QZR3
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Directly resolved unreviewed entry version 36, sequence version 1; SchN1, 379 aa. The HM452329 cross-reference names AEH42483.1. UniProt uses species taxon 1969; its nucleotide-sequence citation identifies strain NRRL 3882. No ortholog substituted.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/AEH42483.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    snippet
    acyl-CoA dehydrogenase
    notes
    AEH42483.1 is encoded by HM452329.1:32036..33175; native Streptomyces chartreusis NRRL 3882, taxon 1079985. Native DNA/code-11 translation, deposited CDS, separately retrieved GenPept and UniProt sequences agree exactly. Deposited product text is an annotation, not a direct substrate-specific catalytic experiment.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/HM452329.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Versioned native cluster sequence supporting the reference identity and gene order.
calN1 (paper locus) was internally replaced with neo in WQL2 calN1::neo disruption The mutation concerns the encoding locus and could affect neighboring expression; it is not a selective perturbation of purified protein activity.
DOI:10.1128/AAC.01130-10
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-11
    notes
    Methods and Figure 5 describe WQL2 from native NRRL 3882 using pJTU3175, double-crossover internal replacement and flanking-PCR confirmation. This was a resistance-cassette insertion, not a scarless deletion.
WQL2 calN1::neo disruption abolished detectable production of calcimycin (A23187) This is a whole-strain production phenotype, not purified-enzyme catalysis, sufficiency or proof that polar effects are excluded. The inspected study complements calN2 in WQL5, not this locus. Figure 1 identifies calcimycin as compound 1 (R1=NHCH3), distinct from cezomycin (R1=H). No cezomycin phenotype is adopted here.
DOI:10.1128/AAC.01130-10
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-11
    notes
    Figure 6 compares WQL2 with native NRRL 3882 and a calcimycin standard. Methods describe SFM cultivation at 30 C for 6-10 days, methanol extraction and HPLC/MS; the displayed chromatograms use UV detection at 280 nm. The calcimycin peak is absent in the mutant under these conditions; no detection limit is supplied.
    snippet
    deletion of each of these three genes abolished the production of calcimycin
SchB2 native reference is the native reference inferred for the product of calB2 (paper locus) The grounded node denotes the native reference protein. The paper locus remains separate; inferred alias correspondence is not an exact assayed-protein sequence claim.
DOI:10.1128/AAC.01130-10
UniProtKB:F8QZR0
https://www.ncbi.nlm.nih.gov/protein/AEH42480.1
https://www.ncbi.nlm.nih.gov/nuccore/HM452329.1
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Table 3 assigns calB2 a 226-aa product. The study deposits HM452329; its ordered gene position, compatible name, length and putative function map to SchB2. This alias mapping does not independently sequence the experimentally disrupted strain.
  • reference
    UniProtKB:F8QZR0
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Directly resolved unreviewed entry version 29, sequence version 1; SchB2, 226 aa. The HM452329 cross-reference names AEH42480.1. UniProt uses species taxon 1969; its nucleotide-sequence citation identifies strain NRRL 3882. No ortholog substituted.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/AEH42480.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    snippet
    isochorismatase
    notes
    AEH42480.1 is encoded by complement(HM452329.1:28948..29628); native Streptomyces chartreusis NRRL 3882, taxon 1079985. Native DNA/code-11 translation, deposited CDS, separately retrieved GenPept and UniProt sequences agree exactly. Deposited product text is an annotation, not a direct substrate-specific catalytic experiment.
  • reference
    https://www.ncbi.nlm.nih.gov/nuccore/HM452329.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Versioned native cluster sequence supporting the reference identity and gene order.
calB2 (paper locus) was internally replaced with neo in WQL4 calB2::neo disruption The mutation concerns the encoding locus and could affect neighboring expression; it is not a selective perturbation of purified protein activity.
DOI:10.1128/AAC.01130-10
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-11
    notes
    Methods and Figure 5 describe WQL4 from native NRRL 3882 using pJTU3178, double-crossover internal replacement and flanking-PCR confirmation. This was a resistance-cassette insertion, not a scarless deletion.
WQL4 calB2::neo disruption abolished detectable production of calcimycin (A23187) This is a whole-strain production phenotype, not purified-enzyme catalysis, sufficiency or proof that polar effects are excluded. The inspected study complements calN2 in WQL5, not this locus. Figure 1 identifies calcimycin as compound 1 (R1=NHCH3), distinct from cezomycin (R1=H). No cezomycin phenotype is adopted here.
DOI:10.1128/AAC.01130-10
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-11
    notes
    Figure 6 compares WQL4 with native NRRL 3882 and a calcimycin standard. Methods describe SFM cultivation at 30 C for 6-10 days, methanol extraction and HPLC/MS; the displayed chromatograms use UV detection at 280 nm. The calcimycin peak is absent in the mutant under these conditions; no detection limit is supplied.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 7 nodes, 6 edges

Five sequence-verified native references map by explicit inference to the starter carrier and four PKSs in the 2011 proposed calcimycin assembly model. Module assignments and order are computational predictions, not individual catalytic experiments. Genomic order is not assembly order. This graph neither makes a physical-complex claim nor transfers the separate calN1/calB2 whole-strain phenotypes to these proteins.

SubjectPredicateObjectNotesEvidence
SchN3 native reference is the native reference inferred for a proposed participant in 2011 proposed carrier-PKS assembly The native protein node is not a purified, modified or engineered assay construct. This edge records a predicted contribution, not demonstrated catalysis, direct binding, a physical complex or an individual knockout phenotype.
DOI:10.1128/AAC.01130-10
DOI:10.1128/AAC.01130-10
https://www.uniprot.org/uniprotkb/F8QZR5/entry
https://www.ncbi.nlm.nih.gov/protein/AEH42485.1
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    COMPUTATIONAL_PREDICTION
    retrieved on
    2026-10-11
    notes
    Figure7A-B proposes a carrier-bound pyrrole starter supplied to the first PKS module. Neither holo/loading state nor starter transfer is individually demonstrated here.
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Table3 assigns CalN3 a 87-aa product. Its deposited HM452329 sequence position, name and annotation support mapping to SchN3. The cal/Sch alias and predicted role are not an assayed-protein identity claim.
  • reference
    https://www.uniprot.org/uniprotkb/F8QZR5/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Unreviewed entry version28, sequence version1: SchN3, 87aa; explicit HM452329/AEH42485.1 cross-reference. UniProt species taxon1969 retains the NRRL3882 nucleotide-reference strain attribution.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/AEH42485.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    snippet
    proline carrier protein
    notes
    AEH42485.1, HM452329.1:34679..34942; NRRL3882, taxon1079985. Native DNA/code11/CDS, independently retrieved GenPept and direct UniProt sequences agree exactly. Deposited product text is an annotation, not a catalytic measurement.
SchA1 native reference is the native reference inferred for a proposed participant in 2011 proposed carrier-PKS assembly The native protein node is not a purified, modified or engineered assay construct. This edge records a predicted contribution, not demonstrated catalysis, direct binding, a physical complex or an individual knockout phenotype.
DOI:10.1128/AAC.01130-10
DOI:10.1128/AAC.01130-10
https://www.uniprot.org/uniprotkb/F8QZQ3/entry
https://www.ncbi.nlm.nih.gov/protein/AEH42473.1
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    COMPUTATIONAL_PREDICTION
    retrieved on
    2026-10-11
    notes
    Table4 and Figure7B assign modules1a/1b to the first two elongations, both using methylmalonyl units, with ketoreduction in the second module. These are domain-based predictions.
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Table3 assigns CalA1 a 2942-aa product. Its deposited HM452329 sequence position, name and annotation support mapping to SchA1. The cal/Sch alias and predicted role are not an assayed-protein identity claim.
  • reference
    https://www.uniprot.org/uniprotkb/F8QZQ3/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Unreviewed entry version60, sequence version1: SchA1, 2942aa; explicit HM452329/AEH42473.1 cross-reference. UniProt species taxon1969 retains the NRRL3882 nucleotide-reference strain attribution.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/AEH42473.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    snippet
    polyketide synthase
    notes
    AEH42473.1, HM452329.1:5100..13928; NRRL3882, taxon1079985. Native DNA/code11/CDS, independently retrieved GenPept and direct UniProt sequences agree exactly. Deposited product text is an annotation, not a catalytic measurement.
SchA2 native reference is the native reference inferred for a proposed participant in 2011 proposed carrier-PKS assembly The native protein node is not a purified, modified or engineered assay construct. This edge records a predicted contribution, not demonstrated catalysis, direct binding, a physical complex or an individual knockout phenotype.
DOI:10.1128/AAC.01130-10
DOI:10.1128/AAC.01130-10
https://www.uniprot.org/uniprotkb/F8QZQ4/entry
https://www.ncbi.nlm.nih.gov/protein/AEH42474.1
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    COMPUTATIONAL_PREDICTION
    retrieved on
    2026-10-11
    notes
    Table4 proposes module3 with a methylmalonyl extender and full beta-carbon reduction. Its placement relative to CalA5b follows the authors' tentative assembly model, not genomic order.
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Table3 assigns CalA2 a 2063-aa product. Its deposited HM452329 sequence position, name and annotation support mapping to SchA2. The cal/Sch alias and predicted role are not an assayed-protein identity claim.
  • reference
    https://www.uniprot.org/uniprotkb/F8QZQ4/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Unreviewed entry version66, sequence version1: SchA2, 2063aa; explicit HM452329/AEH42474.1 cross-reference. UniProt species taxon1969 retains the NRRL3882 nucleotide-reference strain attribution.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/AEH42474.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    snippet
    polyketide synthase
    notes
    AEH42474.1, HM452329.1:14292..20483; NRRL3882, taxon1079985. Native DNA/code11/CDS, independently retrieved GenPept and direct UniProt sequences agree exactly. Deposited product text is an annotation, not a catalytic measurement.
SchA5 native reference is the native reference inferred for a proposed participant in 2011 proposed carrier-PKS assembly The native protein node is not a purified, modified or engineered assay construct. This edge records a predicted contribution, not demonstrated catalysis, direct binding, a physical complex or an individual knockout phenotype.
DOI:10.1128/AAC.01130-10
DOI:10.1128/AAC.01130-10
https://www.uniprot.org/uniprotkb/F8QZS1/entry
https://www.ncbi.nlm.nih.gov/protein/AEH42491.1
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    COMPUTATIONAL_PREDICTION
    retrieved on
    2026-10-11
    notes
    Table4 proposes modules4/5: a malonyl extension with inactive DH/KR, then a methylmalonyl extension with full reduction. Assignment assumes CalA5b follows CalA5a; individual activities and handoffs are not demonstrated.
    snippet
    we tentatively named Cal5a PKS module 4
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Table3 assigns CalA5 a 3956-aa product. Its deposited HM452329 sequence position, name and annotation support mapping to SchA5. The cal/Sch alias and predicted role are not an assayed-protein identity claim.
  • reference
    https://www.uniprot.org/uniprotkb/F8QZS1/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Unreviewed entry version66, sequence version1: SchA5, 3956aa; explicit HM452329/AEH42491.1 cross-reference. UniProt species taxon1969 retains the NRRL3882 nucleotide-reference strain attribution.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/AEH42491.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    snippet
    polyketide synthase
    notes
    AEH42491.1, complement(HM452329.1:48948..60818); NRRL3882, taxon1079985. Native DNA/code11/CDS, independently retrieved GenPept and direct UniProt sequences agree exactly. Deposited product text is an annotation, not a catalytic measurement.
SchA4 native reference is the native reference inferred for a proposed participant in 2011 proposed carrier-PKS assembly The native protein node is not a purified, modified or engineered assay construct. This edge records a predicted contribution, not demonstrated catalysis, direct binding, a physical complex or an individual knockout phenotype.
DOI:10.1128/AAC.01130-10
DOI:10.1128/AAC.01130-10
https://www.uniprot.org/uniprotkb/F8QZS0/entry
https://www.ncbi.nlm.nih.gov/protein/AEH42490.1
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    COMPUTATIONAL_PREDICTION
    retrieved on
    2026-10-11
    notes
    Table4 proposes terminal module6, a malonyl extension with ketoreduction. Absence of a terminal TE domain does not identify the chain-release catalyst; no CalG release or amide-forming role is adopted.
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Table3 assigns CalA4 a 1638-aa product. Its deposited HM452329 sequence position, name and annotation support mapping to SchA4. The cal/Sch alias and predicted role are not an assayed-protein identity claim.
  • reference
    https://www.uniprot.org/uniprotkb/F8QZS0/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Unreviewed entry version60, sequence version1: SchA4, 1638aa; explicit HM452329/AEH42490.1 cross-reference. UniProt species taxon1969 retains the NRRL3882 nucleotide-reference strain attribution.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/AEH42490.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    snippet
    polyketide synthase
    notes
    AEH42490.1, complement(HM452329.1:44006..48922); NRRL3882, taxon1079985. Native DNA/code11/CDS, independently retrieved GenPept and direct UniProt sequences agree exactly. Deposited product text is an annotation, not a catalytic measurement.
2011 proposed carrier-PKS assembly is proposed to contribute the polyketide backbone of calcimycin (A23187) Backbone contribution is not complete calcimycin production or catalytic sufficiency. No intermediate chemical identifier, cezomycin equivalence, CalA3 inactivity/activity, chain-release, benzoxazole formation, spirocyclization or tailoring mechanism is asserted.
DOI:10.1128/AAC.01130-10
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    COMPUTATIONAL_PREDICTION
    retrieved on
    2026-10-11
    notes
    Table4 and Figure7B propose six elongations using four methylmalonyl and two malonyl units, in CalA1-CalA2-CalA5-CalA4 order after carrier-delivered starter loading. The order is a model, not a reconstituted assembly-line experiment.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 6 nodes, 5 edges

Two sequence-verified native references map by explicit inference to CalB3 and CalB4 in the 2011 proposed precursor pathway. The graph separates terminal DHHA aromatization from DAHP entry into a multistep pathway. All functional edges are predictions; no individual activity assay, physical complex or transfer of the separate calB2 knockout phenotype is asserted.

SubjectPredicateObjectNotesEvidence
SchB3 native reference is the native reference inferred for the proposed catalyst of Proposed DHHA aromatization The grounded node denotes the native reference, not a purified or engineered construct. The proposed role is not measured catalysis, a physical complex or an individual knockout phenotype. The separate calB2 disruption result is not transferred to this protein.
DOI:10.1128/AAC.01130-10
DOI:10.1128/AAC.01130-10
https://www.uniprot.org/uniprotkb/F8QZR1/entry
https://www.ncbi.nlm.nih.gov/protein/AEH42481.1
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    COMPUTATIONAL_PREDICTION
    retrieved on
    2026-10-11
    notes
    Figure 7C assigns CalB3 to aromatization of trans-2,3-dihydro-3-hydroxyanthranilic acid (DHHA) to 3-hydroxyanthranilic acid (HA). Table 3 gives a DhbA-like dehydrogenase annotation. This is a homology-based substrate assignment, not a direct substrate-specific assay.
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Table 3 assigns calB3 a 270-aa product. The cited HM452329 deposit's ordered locus, compatible name, length and annotation support mapping to SchB3. The cal/Sch correspondence is an inferred alias, not independently sequenced assay-protein identity.
  • reference
    https://www.uniprot.org/uniprotkb/F8QZR1/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Unreviewed entry version 39, sequence version 1; SchB3, 270 aa; HM452329 cross-reference explicitly names AEH42481.1. Species taxon 1969 is accompanied by the NRRL 3882 nucleotide-reference strain attribution.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/AEH42481.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    snippet
    2,3-dihydroxybenzoate-2,3-dehydrogenase
    notes
    AEH42481.1, complement(HM452329.1:29661..30473); native NRRL 3882, taxon 1079985. Native DNA/code-11/CDS, independently retrieved GenPept and direct UniProt sequences agree. Deposited product text is an annotation, not an experimentally established reaction.
SchB4 native reference is the native reference inferred for the proposed catalyst of Proposed DAHP entry reaction The grounded node denotes the native reference, not a purified or engineered construct. The proposed role is not measured catalysis, a physical complex or an individual knockout phenotype. The separate calB2 disruption result is not transferred to this protein.
DOI:10.1128/AAC.01130-10
DOI:10.1128/AAC.01130-10
https://www.uniprot.org/uniprotkb/F8QZR2/entry
https://www.ncbi.nlm.nih.gov/protein/AEH42482.1
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    COMPUTATIONAL_PREDICTION
    retrieved on
    2026-10-11
    notes
    Figure 7C assigns CalB4 to conversion of phosphoenolpyruvate (PEP) and erythrose-4-phosphate (E4P) to DAHP. Table 3 gives a PhzC-like DAHP-synthase annotation. CalB4 is not assigned all intervening reactions to chorismate or HA.
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Table 3 assigns calB4 a 404-aa product. The cited HM452329 deposit's ordered locus, compatible name, length and annotation support mapping to SchB4. The cal/Sch correspondence is an inferred alias, not independently sequenced assay-protein identity.
  • reference
    https://www.uniprot.org/uniprotkb/F8QZR2/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Unreviewed entry version 29, sequence version 1; SchB4, 404 aa; HM452329 cross-reference explicitly names AEH42482.1. Species taxon 1969 is accompanied by the NRRL 3882 nucleotide-reference strain attribution.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/AEH42482.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    snippet
    3-deoxy-D-arabino-heptulosonate 7-phosphate
    notes
    AEH42482.1, complement(HM452329.1:30511..31725); native NRRL 3882, taxon 1079985. Native DNA/code-11/CDS, independently retrieved GenPept and direct UniProt sequences agree. Deposited product text is an annotation, not an experimentally established reaction.
Proposed DHHA aromatization is proposed as the terminal reaction in Proposed 3-hydroxyanthranilate supply No cofactor specificity, kinetic value, physiological flux or direct enzyme assay is asserted.
DOI:10.1128/AAC.01130-10
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    COMPUTATIONAL_PREDICTION
    retrieved on
    2026-10-11
    notes
    Figure 7C places CalB3 on the DHHA-to-HA arrow. This is the terminal reaction of the drawn precursor-supply model, not the terminal reaction of calcimycin biosynthesis.
Proposed DAHP entry reaction is proposed to feed the multistep pathway for Proposed 3-hydroxyanthranilate supply This is an indirect multistep contribution, not direct CalB4 production of chorismate or HA. No additional enzymes are invented for unlabeled steps; the CalB1 reference-length discrepancy remains unresolved.
DOI:10.1128/AAC.01130-10
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    COMPUTATIONAL_PREDICTION
    retrieved on
    2026-10-11
    notes
    Figure 7C places DAHP upstream of chorismate, followed by the proposed CalB1/CalB2/CalB3 route to HA. Its intervening unlabeled arrows are not assigned to genes in the depicted cluster.
Proposed 3-hydroxyanthranilate supply is proposed to provide the benzoxazole building block for calcimycin (A23187) Precursor supply is not benzoxazole ring closure, attachment to the PKS chain or sufficiency for final-product formation. No exact intermediate chemical identifier, cezomycin equivalence, CalG termination or downstream tailoring step is adopted.
DOI:10.1128/AAC.01130-10
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    COMPUTATIONAL_PREDICTION
    retrieved on
    2026-10-11
    notes
    The 2011 prediction section and Figure 7C place the CalB route upstream of the benzoxazole portion of calcimycin. The older feeding experiments cited in its Discussion are not recast as directly inspected experiments on these two proteins.
    snippet
    The proposed gene assignment for the pathway

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 8 nodes, 10 edges

Native SchM grounding, cassette genetics/complementation and recombinant His6-CalM methylation are separate claims. Main-text experimental results are adopted with explicit construct and figure/SI limits; the N-demethyl substrate remains chemically ungrounded.

SubjectPredicateObjectNotesEvidence
SchM native reference is the native reference inferred for the product of calM (paper locus) Native reference identity is separate from the tagged assay protein. No exact full fusion sequence, physiological complex or untagged-native activity measurement is asserted.
DOI:10.1128/AAC.01130-10
DOI:10.1016/j.biochi.2013.03.014
https://www.uniprot.org/uniprotkb/F8QZQ7/entry
https://www.ncbi.nlm.nih.gov/protein/AEH42477.1
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Table3 assigns calM a 229-aa product. Its ordered HM452329 locus, length and compatible annotation support the inferred alias SchM.
  • reference
    DOI:10.1016/j.biochi.2013.03.014
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    The 2013 study cites HM452329 and a229-aa CalM. Both printed pJTU3182 cloning primers uniquely match native SchM CDS ends. This supports the alias and template, not an independently sequenced complete plasmid or vector-derived fusion.
  • reference
    https://www.uniprot.org/uniprotkb/F8QZQ7/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Directly resolved unreviewed entry version33, sequence version1; SchM,229aa. Species taxon1969 retains the NRRL3882 nucleotide-reference strain attribution and HM452329/AEH42477.1 cross-reference.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/AEH42477.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    AEH42477.1,complement(HM452329.1:22502..23191); native NRRL3882,taxon1079985. Native DNA/code11/CDS, independent GenPept and current direct UniProt sequences agree. Product text is a deposited annotation.
    snippet
    N-methyl-transferase
calM (paper locus) was replaced with neo in WQL3 calM::neo disruption A whole-locus perturbation is not a selective inhibition of purified CalM and does not by itself exclude polar effects.
DOI:10.1016/j.biochi.2013.03.014
  • reference
    DOI:10.1016/j.biochi.2013.03.014
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-11
    notes
    Methods2.2 describes pJTU3179, double-crossover selection and PCR confirmation of WQL3. This was resistance-cassette replacement, not scarless deletion.
WQL3 calM::neo disruption lost detectable production of calcimycin (A23187) Reported main-text phenotype; figure pixels and detection limits were not independently inspected. No numerical zero yield asserted.
DOI:10.1016/j.biochi.2013.03.014
  • reference
    DOI:10.1016/j.biochi.2013.03.014
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-11
    notes
    Methods2.3/Results3.3 report HPLC/MS after solid SFM cultivation at30C for6-10days, with a Sigma calcimycin standard. WQL3 accumulated the N-demethyl product instead of calcimycin.
WQL3 calM::neo disruption accumulated the product assigned as N-demethylcalcimycin (paper substrate) Paper-assigned chemical identity only. Supplementary spectra are uninspected; no exact chemical identifier, atom map or equivalence to cezomycin is adopted.
DOI:10.1016/j.biochi.2013.03.014
  • reference
    DOI:10.1016/j.biochi.2013.03.014
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-11
    notes
    Results3.2-3.3 assign the accumulated compound by LC/MS and report supporting NMR in the supplement.
calM (paper locus) was carried on the complementation fragment supplied in WQL6 calM-fragment complementation Fragment boundaries and complete construct sequence were not independently checked. This is complementation, not scarless repair or proof that no adjacent sequence contributed.
DOI:10.1016/j.biochi.2013.03.014
  • reference
    DOI:10.1016/j.biochi.2013.03.014
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-11
    notes
    Methods2.2 describes a BamHI fragment from pJTU3166 carrying calM in pSET152-derived pJTU3187, introduced into WQL3. Results3.3 describes its natural promoter.
WQL6 calM-fragment complementation restored reported production of calcimycin (A23187) Restored detection is not a measured equality of yield, expression level or pathway flux.
DOI:10.1016/j.biochi.2013.03.014
  • reference
    DOI:10.1016/j.biochi.2013.03.014
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-11
    notes
    Results3.3 reports calcimycin production in WQL6 and LC/MS confirmation of the HPLC peak.
    snippet
    The resulting strain, WQL6, produced calcimycin.
SchM native reference provides the native sequence reference inferred for His6-CalM (paper construct) Native reference identity is separate from the tagged assay protein. No exact full fusion sequence, physiological complex or untagged-native activity measurement is asserted.
DOI:10.1016/j.biochi.2013.03.014
  • reference
    DOI:10.1016/j.biochi.2013.03.014
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Methods2.4 clones the genomic calM insert into pET28a at BamHI/HindIII as pJTU3182, expressed in E.coli BL21(DE3)/pLysE and purified by nickel affinity. Printed primer ends match SchM; vector-derived residues remain unverified.
His6-CalM (paper construct) catalyzed N-methylation in SAM-dependent CalM assay This edge concerns the reported purified tagged construct and assay, not an independently sequenced native enzyme. No kinetics, substrate breadth or physiological sufficiency claimed.
DOI:10.1016/j.biochi.2013.03.014
  • reference
    DOI:10.1016/j.biochi.2013.03.014
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-11
    notes
    Methods2.6/Results3.4:50uL,75mM Tris-HCl pH8.0,1mM SAM,2%DMSO,0.5mM substrate and1.6uM CalM;37C,15min, methanol quench and HPLC/MS. The reported negative control omitted SAM.
    snippet
    CalM is indeed an N-methyltransferase
N-demethylcalcimycin (paper substrate) was the reported substrate in SAM-dependent CalM assay Retain the ungrounded paper substrate; do not substitute cezomycin or silently resolve its distinct stereochemical questions.
DOI:10.1016/j.biochi.2013.03.014
  • reference
    DOI:10.1016/j.biochi.2013.03.014
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-11
    notes
    Methods2.5-2.6 uses the isolated WQL3-derived N-demethyl compound in the recombinant enzyme assay.
SAM-dependent CalM assay formed a product assigned as calcimycin (A23187) Reported main-text assay outcome, not a new inspection of Figure5 or supplementary spectra. No upstream ring-closure, activation or amination step is inferred.
DOI:10.1016/j.biochi.2013.03.014
  • reference
    DOI:10.1016/j.biochi.2013.03.014
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-11
    notes
    Results3.4 reports calcimycin formation confirmed by mass spectrometry, contrasting with the no-SAM control.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 5 nodes, 5 edges

Sequence-verified native SchR3 is mapped by inference to the paper locus. Separate experimental edges describe cassette disruption, increased calcimycin production and complementation. No assay construct, export activity or small-molecule mechanism is inferred from this phenotype alone.

SubjectPredicateObjectNotesEvidence
SchR3 native reference is the native reference inferred for the product of calR3 (paper locus) Native reference identity is separate from the tagged assay protein. No exact full fusion sequence, physiological complex or untagged-native activity measurement is asserted.
DOI:10.1128/AAC.01130-10
DOI:10.3389/fmicb.2017.02371
https://www.uniprot.org/uniprotkb/F8QZR9/entry
https://www.ncbi.nlm.nih.gov/protein/AEH42489.1
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Table3 assigns calR3 a 211-aa product. Its ordered HM452329 locus, length and compatible annotation support the inferred alias SchR3.
  • reference
    DOI:10.3389/fmicb.2017.02371
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    The 2017 study cites HM452329 and NRRL3882, targeting the calR3/calT neighborhood. Ordered loci and the 2011 length assignments support cal/Sch aliases. Held primer/figure details do not establish complete mutant, probe or recombinant construct sequences.
  • reference
    https://www.uniprot.org/uniprotkb/F8QZR9/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Directly resolved unreviewed entry version51, sequence version1; SchR3,211aa. Species taxon1969 retains the NRRL3882 nucleotide-reference strain attribution and HM452329/AEH42489.1 cross-reference.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/AEH42489.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    AEH42489.1,HM452329.1:43244..43879; native NRRL3882,taxon1079985. Native DNA/code11/CDS, independent GenPept and current direct UniProt sequences agree. Product text is a deposited annotation.
    snippet
    TetR family regulator
calR3 (paper locus) was internally replaced by aac(3)IV-oriT in GLX26 calR3 cassette disruption Cassette disruption, not scarless deletion. Exact endpoints remain ungrounded because primer sequences and figure pixels were not inspected.
DOI:10.3389/fmicb.2017.02371
  • reference
    DOI:10.3389/fmicb.2017.02371
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-11
    notes
    Methods/Results describe pJTU3791 and a455bp internal replacement, double-crossover selection and PCR confirmation. The reported PCR products are714bp for WT and1643bp for GLX26.
    snippet
    an internal 455-bp DNA sequence in calR3 was replaced
GLX26 calR3 cassette disruption increased reported production of calcimycin (A23187) Whole-strain production phenotype, not direct CalT transport or a catalytic measurement. Figure2 pixels, error estimates and quantitative normalization are uninspected. Growth measurements in TSBY are not treated as exclusion of every growth confound in solid SFM.
DOI:10.3389/fmicb.2017.02371
  • reference
    DOI:10.3389/fmicb.2017.02371
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-11
    notes
    Main Results report approximately8-fold higher calcimycin yield by HPLC than WT. Methods specify solid SFM,30C,7days.
calR3 (paper locus) was supplied on pJTU3795 in GLX29 calR3 complementation Complementation rather than scarless repair. Primer sequences and the complete insert remain uninspected; native promoter identity, dosage and neighboring effects are not assumed.
DOI:10.3389/fmicb.2017.02371
  • reference
    DOI:10.3389/fmicb.2017.02371
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-11
    notes
    Methods describe an intact genomic calR3 PCR fragment in integrating pJTU2170-derived pJTU3795, introduced into GLX26.
GLX29 calR3 complementation was reported to restore the wild-type production pattern of calcimycin (A23187) Qualitative main-text rescue claim only; no exact equality of yield or independently inspected chromatogram. Cezomycin outcomes are not imported.
DOI:10.3389/fmicb.2017.02371
  • reference
    DOI:10.3389/fmicb.2017.02371
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-11
    notes
    Results and Figure2 caption report restoration of production after calR3 complementation.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 7 nodes, 6 edges

Native references and a tagged DNA-binding assay support an explicitly inferred transcriptional model. CalT export remains the authors' unproven hypothesis, separate from CalR3 perturbation phenotypes. RNA signal, protein abundance, DNA binding and small-molecule transport are not interchangeable readouts.

SubjectPredicateObjectNotesEvidence
SchR3 native reference provides the native sequence reference inferred for His6-CalR3 (paper construct) Native reference identity is separate from the tagged assay protein. No exact full fusion sequence, physiological complex or untagged-native activity measurement is asserted.
DOI:10.1128/AAC.01130-10
DOI:10.3389/fmicb.2017.02371
https://www.uniprot.org/uniprotkb/F8QZR9/entry
https://www.ncbi.nlm.nih.gov/protein/AEH42489.1
DOI:10.3389/fmicb.2017.02371
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Table3 assigns calR3 a 211-aa product. Its ordered HM452329 locus, length and compatible annotation support the inferred alias SchR3.
  • reference
    DOI:10.3389/fmicb.2017.02371
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    The 2017 study cites HM452329 and NRRL3882, targeting the calR3/calT neighborhood. Ordered loci and the 2011 length assignments support cal/Sch aliases. Held primer/figure details do not establish complete mutant, probe or recombinant construct sequences.
  • reference
    https://www.uniprot.org/uniprotkb/F8QZR9/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Directly resolved unreviewed entry version51, sequence version1; SchR3,211aa. Species taxon1969 retains the NRRL3882 nucleotide-reference strain attribution and HM452329/AEH42489.1 cross-reference.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/AEH42489.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    AEH42489.1,HM452329.1:43244..43879; native NRRL3882,taxon1079985. Native DNA/code11/CDS, independent GenPept and current direct UniProt sequences agree. Product text is a deposited annotation.
    snippet
    TetR family regulator
  • reference
    DOI:10.3389/fmicb.2017.02371
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Methods reports the intact genomic gene cloned at NdeI/EcoRI in pET28a-calR3, sequence-validated by the authors, expressed in BL21(DE3)/pLysE and purified as N-terminal His6-CalR3. The actual insert/tag sequence was not independently inspected.
His6-CalR3 (paper construct) bound in the reported in-vitro assay to T-R3L promoter probe (paper) No exact probe sequence/coordinates, binding-site bases, stoichiometry, dimer or affinity value is adopted. Figure pixels and primer supplement remain uninspected.
DOI:10.3389/fmicb.2017.02371
  • reference
    DOI:10.3389/fmicb.2017.02371
    evidence type
    PRIMARY_EXPERIMENT
    retrieved on
    2026-10-11
    notes
    Main Methods/Results describe EMSA on the calT-calR3 intergenic probes, binding to T-R3L, and DNaseI protection. EMSA:37C,30min,20uL,20mM Tris-Cl,100mM KCl,10mM DTT,pH8.0.
SchR3 native reference is inferred to repress calR3 transcription (regulatory model) Regulatory interpretation, not reconstituted transcription. Primer/amplicon boundaries in the455bp cassette mutant are uninspected; the signal is not proof of intact wild-type calR3 mRNA or increased CalR3 protein in GLX26.
DOI:10.3389/fmicb.2017.02371
  • reference
    DOI:10.3389/fmicb.2017.02371
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Authors combine genomic-locus perturbation,96h TSBY RT-qPCR normalized to16S rDNA, recombinant DNA binding and reported TSP positions to interpret autorepression. The reported calR3 RT-qPCR signal rises about30-fold in GLX26.
SchR3 native reference is inferred to repress calT transcription (regulatory model) Transcript signal is not CalT protein abundance or a transport measurement. No exact promoter boundaries or reconstructed transcription assay is asserted.
DOI:10.3389/fmicb.2017.02371
  • reference
    DOI:10.3389/fmicb.2017.02371
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    The same combined evidence supports the authors' calT repression model. Main Results report about171-fold higher calT RT-qPCR signal in GLX26 at96h; the study uses16S rDNA normalization.
SchT native reference is the native reference inferred for the product encoded at the locus measured by calT transcription (regulatory model) Grounding the gene product does not turn the RNA readout into protein abundance or prove the annotated transporter substrate.
DOI:10.1128/AAC.01130-10
DOI:10.3389/fmicb.2017.02371
https://www.uniprot.org/uniprotkb/F8QZR8/entry
https://www.ncbi.nlm.nih.gov/protein/AEH42488.1
  • reference
    DOI:10.1128/AAC.01130-10
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Table3 assigns calT a 749-aa product. Its ordered HM452329 locus, length and compatible annotation support the inferred alias SchT.
  • reference
    DOI:10.3389/fmicb.2017.02371
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    The 2017 study cites HM452329 and NRRL3882, targeting the calR3/calT neighborhood. Ordered loci and the 2011 length assignments support cal/Sch aliases. Held primer/figure details do not establish complete mutant, probe or recombinant construct sequences.
  • reference
    https://www.uniprot.org/uniprotkb/F8QZR8/entry
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    Directly resolved unreviewed entry version25, sequence version1; SchT,749aa. Species taxon1969 retains the NRRL3882 nucleotide-reference strain attribution and HM452329/AEH42488.1 cross-reference.
  • reference
    https://www.ncbi.nlm.nih.gov/protein/AEH42488.1
    evidence type
    DATABASE_ASSERTION
    retrieved on
    2026-10-11
    notes
    AEH42488.1,complement(HM452329.1:40840..43089); native NRRL3882,taxon1079985. Native DNA/code11/CDS, independent GenPept and current direct UniProt sequences agree. Product text is a deposited annotation.
    snippet
    transmembrane transport protein
SchT native reference was proposed to export calcimycin (A23187) Explicit hypothesis, not measured flux, directionality, substrate specificity, energetic coupling or self-resistance. Whole-strain extracellular/intracellular yields do not isolate CalT activity; the Methods/Results medium descriptions differ, so no quantitative partition ratio is imported. No ligand-affinity, allostery or cezomycin chemistry claim is adopted.
DOI:10.3389/fmicb.2017.02371
  • reference
    DOI:10.3389/fmicb.2017.02371
    evidence type
    CURATOR_INFERENCE
    retrieved on
    2026-10-11
    notes
    Discussion proposes CalT-mediated export from transporter-family similarity and indirect calR3-disruption/transcript/partitioning observations. No direct CalT transport experiment is described.
    snippet
    CalT may be involved in expulsion of calcimycin

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Explore across X-Mech

AntibioticMech (A23187) ↗ MediaIngredientMech (naturalproductmech:mibig-18de581c96) ↗ TaxonMech: Streptomyces chartreusis NRRL 3882 (NCBITaxon:1079985) ↗ PathwayMech (mibig:BGC0000032) ↗

Open questions 5

curator-calcimycin-reference-and-experiment-boundaries — Can exact experimental sequences and individual protein functions resolve the remaining calcimycin reference, locus and construct boundaries?

curator-calcimycin-pks-prediction-boundaries — Which direct experiments establish starter transfer, module specificity and ordered handoffs for the proposed calcimycin carrier-PKS assembly?

curator-calcimycin-precursor-prediction-boundaries — Do sequence-verified CalB3 and CalB4 catalyze the proposed precursor reactions in vivo and in vitro?

curator-calcimycin-calm-construct-and-chemical-scope — Can the complete CalM construct and substrate/product spectra independently resolve the remaining assay boundaries?

curator-calcimycin-regulatory-assay-and-export-boundaries — Which construct, probe, RT-qPCR amplicon and direct transport data test the CalR3/CalT regulatory model?

Provenance

Source conceptSourceVersion
BGC0000032MIBIG5

This page is generated from data/natural_products/polyketides/a23187.yaml, which is generated from the committed inventories. Neither is edited by hand.