asperfuranone

naturalproductmech:mibig-3358de9614 MINTED SEEDED

Grounding review: No 3-star ChEBI entry shares this structure, so the record keeps a content-hashed identifier.

What these status tags mean

Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.

Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.

Structure

Standard InChIKey	ZNGSMORVYUMUDS-UHFFFAOYSA-N
SMILES	CCC(C)C=C(C)C=CC(=O)C1=C2CC(O)C(C)(O)C(=O)C2=CO1
Stereochemistry	undefined stereocentres — the InChIKey does not distinguish this compound from its stereoisomers
Cross-references	npatlas:NPA010580

Filing pathway

Polyketides — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is pks, from MIBiG.

Producer organisms 1

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

Taxon	Basis	Cluster	Evidence
Aspergillus nidulans FGSC A4 NCBITaxon:227321	SOURCE_ASSERTION	mibig:BGC0000022	DOI:10.1111/j.1742-7843.2010.00545.x

Occurrences 0

None cited.

Biosynthetic gene clusters 1

Accession	Host	Locus evidence	Genome
mibig:BGC0000022	Aspergillus nidulans FGSC A4 NCBITaxon:227321	CLUSTER_UNSTATED	genbank:AACD01000015.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Mechanism & Biosynthesis Overview

Causal graph — biosynthesis 22 nodes, 20 edges

Partial genetic-perturbation view; mediation and chemical reaction steps are intentionally omitted. The 2009 alcAp-afoA experiments used an nkuA-deletion/stcJ-deletion background; AfoA was induced, not deleted. The 2010 ScpR experiments are abstract-bounded, including afoA deletion during ScpR induction. Shared outcome nodes denote readout categories, not pooled cultures. FGSC A4 accessions are native references, not verified experimental alleles. Claims concern asperfuranone at this owner's stereo-unspecified resolution, not every possible stereoisomer. No direct promoter binding, transport substrate, catalytic order or universal phenotype is asserted. The 2009 Results, Table 1, captions and partial Methods were inspected; figure images, detailed spectra and supplement were not. No primary isomer-level identity sign-off is claimed. The added2010 negative-control condition combines inpB deletion with alcAp-scpR induction and retains asperfuranone detection. It does not establish universal InpB dispensability, a quantitative yield, or direct ScpR promoter binding.

Subject	Predicate	Object	Notes	Evidence
AfoA (FGSC A4 native reference)	is_native_reference_for_targeted_gene_in	alcAp-afoA induction	Native reference identity only, not a verified experimental allele, measured protein abundance or direct molecular activity.	https://www.uniprot.org/uniprotkb/Q5BEK1/entry https://www.ncbi.nlm.nih.gov/protein/CBF88304.1 reference https://www.uniprot.org/uniprotkb/Q5BEK1/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes Reviewed afoA/ANIA_01029, FGSC A4 taxon227321; entry 127, sequence 1, annotation 2026-09-02. This database identity mapping is separate from the primary outcome evidence. reference https://www.ncbi.nlm.nih.gov/protein/CBF88304.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes CBF88304.1, 666 aa: complete spliced CDS on BN001308.1:1698501..1700854 (4 exon(s), strand 1), GenPept translation and UniProt sequence agree, including start/stop. Native FGSC A4; experimental strain and allele equivalence remain unverified.
alcAp-afoA induction	results_in	Asperfuranone production detected in the indicated experiment		DOI:10.1021/ja8088185 reference DOI:10.1021/ja8088185 evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-12 notes Results/Discussion; experimental details and inspection limits are qualified in the graph description.
AfoB (FGSC A4 native reference)	is_native_reference_for_targeted_gene_in	afoB deletion under alcAp-afoA induction	Native reference identity only, not a verified experimental allele, measured protein abundance or direct molecular activity.	https://www.uniprot.org/uniprotkb/Q5BEJ9/entry https://www.ncbi.nlm.nih.gov/protein/CBF88300.1 reference https://www.uniprot.org/uniprotkb/Q5BEJ9/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes Reviewed afoB/ANIA_01031, FGSC A4 taxon227321; entry 106, sequence 1, annotation 2026-09-02. This database identity mapping is separate from the primary outcome evidence. reference https://www.ncbi.nlm.nih.gov/protein/CBF88300.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes CBF88300.1, 538 aa: complete spliced CDS on BN001308.1:1693289..1695321 (6 exon(s), strand 1), GenPept translation and UniProt sequence agree, including start/stop. Native FGSC A4; experimental strain and allele equivalence remain unverified.
afoB deletion under alcAp-afoA induction	results_in	Asperfuranone production strongly diminished in the indicated experiment		DOI:10.1021/ja8088185 reference DOI:10.1021/ja8088185 evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-12 notes Results/Discussion; experimental details and inspection limits are qualified in the graph description. snippet strongly diminished production
AfoC (FGSC A4 native reference)	is_native_reference_for_targeted_gene_in	afoC deletion under alcAp-afoA induction	Native reference identity only, not a verified experimental allele, measured protein abundance or direct molecular activity.	https://www.uniprot.org/uniprotkb/Q5BEJ8/entry https://www.ncbi.nlm.nih.gov/protein/CBF88298.1 reference https://www.uniprot.org/uniprotkb/Q5BEJ8/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes Reviewed afoC/ANIA_01032, FGSC A4 taxon227321; entry 106, sequence 1, annotation 2026-09-02. This database identity mapping is separate from the primary outcome evidence. reference https://www.ncbi.nlm.nih.gov/protein/CBF88298.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes CBF88298.1, 297 aa: complete spliced CDS on BN001308.1:1692011..1692904 (1 exon(s), strand 1), GenPept translation and UniProt sequence agree, including start/stop. Native FGSC A4; experimental strain and allele equivalence remain unverified.
afoC deletion under alcAp-afoA induction	results_in	Asperfuranone production strongly diminished in the indicated experiment		DOI:10.1021/ja8088185 reference DOI:10.1021/ja8088185 evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-12 notes Results/Discussion; experimental details and inspection limits are qualified in the graph description.
AfoD (FGSC A4 native reference)	is_native_reference_for_targeted_gene_in	afoD deletion under alcAp-afoA induction	Native reference identity only, not a verified experimental allele, measured protein abundance or direct molecular activity.	https://www.uniprot.org/uniprotkb/Q5BEJ7/entry https://www.ncbi.nlm.nih.gov/protein/CBF88297.1 reference https://www.uniprot.org/uniprotkb/Q5BEJ7/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes Reviewed afoD/ANIA_01033, FGSC A4 taxon227321; entry 116, sequence 1, annotation 2026-09-02. This database identity mapping is separate from the primary outcome evidence. reference https://www.ncbi.nlm.nih.gov/protein/CBF88297.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes CBF88297.1, 440 aa: complete spliced CDS on BN001308.1:1689954..1691405 (3 exon(s), strand -1), GenPept translation and UniProt sequence agree, including start/stop. Native FGSC A4; experimental strain and allele equivalence remain unverified.
afoD deletion under alcAp-afoA induction	results_in	Asperfuranone production abolished in the indicated experiment		DOI:10.1021/ja8088185 reference DOI:10.1021/ja8088185 evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-12 notes Results/Discussion; experimental details and inspection limits are qualified in the graph description. snippet fully eliminated asperfuranone (1) production
AfoE (FGSC A4 native reference)	is_native_reference_for_targeted_gene_in	afoE deletion under alcAp-afoA induction	Native reference identity only, not a verified experimental allele, measured protein abundance or direct molecular activity.	https://www.uniprot.org/uniprotkb/Q5BEJ6/entry https://www.ncbi.nlm.nih.gov/protein/CBF88295.1 reference https://www.uniprot.org/uniprotkb/Q5BEJ6/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes Reviewed afoE/ANIA_01034, FGSC A4 taxon227321; entry 144, sequence 1, annotation 2026-09-02. This database identity mapping is separate from the primary outcome evidence. reference https://www.ncbi.nlm.nih.gov/protein/CBF88295.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes CBF88295.1, 2793 aa: complete spliced CDS on BN001308.1:1680174..1689104 (3 exon(s), strand -1), GenPept translation and UniProt sequence agree, including start/stop. Native FGSC A4; experimental strain and allele equivalence remain unverified.
afoE deletion under alcAp-afoA induction	results_in	Asperfuranone production abolished in the indicated experiment		DOI:10.1021/ja8088185 reference DOI:10.1021/ja8088185 evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-12 notes Results/Discussion; experimental details and inspection limits are qualified in the graph description.
AfoF (FGSC A4 native reference)	is_native_reference_for_targeted_gene_in	afoF deletion under alcAp-afoA induction	Native reference identity only, not a verified experimental allele, measured protein abundance or direct molecular activity.	https://www.uniprot.org/uniprotkb/Q5BEJ5/entry https://www.ncbi.nlm.nih.gov/protein/CBF88293.1 reference https://www.uniprot.org/uniprotkb/Q5BEJ5/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes Reviewed afoF/ANIA_01035, FGSC A4 taxon227321; entry 105, sequence 1, annotation 2026-09-02. This database identity mapping is separate from the primary outcome evidence. reference https://www.ncbi.nlm.nih.gov/protein/CBF88293.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes CBF88293.1, 481 aa: complete spliced CDS on BN001308.1:1678054..1679646 (3 exon(s), strand -1), GenPept translation and UniProt sequence agree, including start/stop. Native FGSC A4; experimental strain and allele equivalence remain unverified.
afoF deletion under alcAp-afoA induction	results_in	Asperfuranone production abolished in the indicated experiment		DOI:10.1021/ja8088185 reference DOI:10.1021/ja8088185 evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-12 notes Results/Discussion; experimental details and inspection limits are qualified in the graph description.
AfoG (FGSC A4 native reference)	is_native_reference_for_targeted_gene_in	afoG deletion under alcAp-afoA induction	Native reference identity only, not a verified experimental allele, measured protein abundance or direct molecular activity.	https://www.uniprot.org/uniprotkb/Q5BEJ4/entry https://www.ncbi.nlm.nih.gov/protein/CBF88289.1 reference https://www.uniprot.org/uniprotkb/Q5BEJ4/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes Reviewed afoG/ANIA_01036, FGSC A4 taxon227321; entry 152, sequence 1, annotation 2026-09-02. This database identity mapping is separate from the primary outcome evidence. reference https://www.ncbi.nlm.nih.gov/protein/CBF88289.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes CBF88289.1, 2527 aa: complete spliced CDS on BN001308.1:1668237..1676285 (5 exon(s), strand 1), GenPept translation and UniProt sequence agree, including start/stop. Native FGSC A4; experimental strain and allele equivalence remain unverified.
afoG deletion under alcAp-afoA induction	results_in	Asperfuranone production abolished in the indicated experiment		DOI:10.1021/ja8088185 reference DOI:10.1021/ja8088185 evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-12 notes Results/Discussion; experimental details and inspection limits are qualified in the graph description.
ScpR (FGSC A4 native reference)	is_native_reference_for_targeted_gene_in	alcAp-scpR induction	Native reference identity only, not a verified experimental allele, measured protein abundance or direct molecular activity.	https://www.uniprot.org/uniprotkb/Q5B7I8/entry https://www.ncbi.nlm.nih.gov/protein/CBF76044.1 reference https://www.uniprot.org/uniprotkb/Q5B7I8/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes Reviewed scpR/ANIA_03492, FGSC A4 taxon227321; entry 94, sequence 1, annotation 2026-09-02. This database identity mapping is separate from the primary outcome evidence. reference https://www.ncbi.nlm.nih.gov/protein/CBF76044.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes CBF76044.1, 277 aa: complete spliced CDS on BN001302.1:3941131..3941964 (1 exon(s), strand -1), GenPept translation and UniProt sequence agree, including start/stop. Native FGSC A4; experimental strain and allele equivalence remain unverified.
alcAp-scpR induction	results_in	Asperfuranone production detected in the indicated experiment		DOI:10.1128/aem.00683-10 reference DOI:10.1128/aem.00683-10 evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-12 notes Author abstract only, indexed at https://europepmc.org/article/MED/20952652. Full Methods and figures were not inspected; inferred promoter contact is not adopted as direct binding. snippet revealed the production of the polyketide asperfuranone
AfoA (FGSC A4 native reference)	is_native_reference_for_targeted_gene_in	afoA deletion under alcAp-scpR induction	Native reference identity only, not a verified experimental allele, measured protein abundance or direct molecular activity.	https://www.uniprot.org/uniprotkb/Q5BEK1/entry https://www.ncbi.nlm.nih.gov/protein/CBF88304.1 reference https://www.uniprot.org/uniprotkb/Q5BEK1/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes Reviewed afoA/ANIA_01029, FGSC A4 taxon227321; entry 127, sequence 1, annotation 2026-09-02. This database identity mapping is separate from the primary outcome evidence. reference https://www.ncbi.nlm.nih.gov/protein/CBF88304.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes CBF88304.1, 666 aa: complete spliced CDS on BN001308.1:1698501..1700854 (4 exon(s), strand 1), GenPept translation and UniProt sequence agree, including start/stop. Native FGSC A4; experimental strain and allele equivalence remain unverified.
afoA deletion under alcAp-scpR induction	results_in	Asperfuranone production abolished in the indicated experiment		DOI:10.1128/aem.00683-10 reference DOI:10.1128/aem.00683-10 evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-12 notes Author abstract only, indexed at https://europepmc.org/article/MED/20952652. Full Methods and figures were not inspected; inferred promoter contact is not adopted as direct binding. snippet was abolished after the deletion of the asperfuranone activator gene afoA
InpB (FGSC A4 native reference)	is_native_reference_for_targeted_gene_in	inpB deletion under alcAp-scpR induction		https://www.uniprot.org/uniprotkb/Q5B7I4/entry https://www.ncbi.nlm.nih.gov/protein/CBF76036.1 reference https://www.uniprot.org/uniprotkb/Q5B7I4/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes Reviewed inpB/ANIA_03496, FGSC A4 taxon227321; entry128, sequence1, annotation2026-09-02. Native reference for the named deletion, not the deletion allele or an asperfuranone catalytic enzyme. reference https://www.ncbi.nlm.nih.gov/protein/CBF76036.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes CBF76036.1,2326aa; complete two-exon reverse-strand CDS on BN001302.1:3924481..3931509 agrees with GenPept and UniProt including start/stop. Previously retrieved native sequences rechecked; experimental strain/allele identity remains unverified.
inpB deletion under alcAp-scpR induction	results_in	Asperfuranone production detected in the indicated experiment		DOI:10.1128/aem.00683-10 reference DOI:10.1128/aem.00683-10 evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-12 notes Previously inspected author abstract at https://europepmc.org/article/MED/20952652 describes inpB deletion plus the alcAp-scpR cassette and detects asperfuranone under inducing conditions. This is a conditional negative-control result, not evidence of equal yield, universal gene dispensability, lack of any biochemical role, or direct promoter contact. Full Methods and exact alleles remain uninspected. snippet the existence of the polyketide product asperfuranone

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Explore across X-Mech

AntibioticMech (asperfuranone) ↗ MediaIngredientMech (naturalproductmech:mibig-3358de9614) ↗ TaxonMech: Aspergillus nidulans FGSC A4 (NCBITaxon:227321) ↗ PathwayMech (mibig:BGC0000022) ↗

Open questions 1

curator-asperfuranone-identity-and-experimental-scope — Can primary stereochemical detail, experimental alleles and molecular mediation be resolved without conflating reference sequences and perturbations?

Provenance

Source concept	Source	Version
BGC0000022	MIBIG	3

This page is generated from data/natural_products/polyketides/asperfuranone.yaml, which is generated from the committed inventories. Neither is edited by hand.