asperthecin

CHEBI:64161 EXACT SEEDED

What these status tags mean

Curation status describes a workflow state. SEEDED means the record entered through the source-seeding workflow; it does not imply that no later curation occurred. PROPOSED marks an addition awaiting review; REVIEWED records curator sign-off on identity, structure, filing class and producer claims; DEPRECATED retains a superseded or out-of-scope record. These are workflow decisions, not independently verified human review. Agent-assisted contributions may appear in the record history.

Grounding describes the identifier mapping: EXACT uses an ontology identity asserted by the source; MINTED keeps a local identifier when no defensible ontology identity is available; REVIEW_NEEDED flags an exact structure whose ontology identity needs adjudication. The grounding note retains any unresolved alternatives. These tags do not establish the strength of producer or activity evidence.

An anthraquinone pigment obtained from the mould Aspergillus nidulans.

Structure

Standard InChIKey	DLOLMYKOOZLTPY-UHFFFAOYSA-N
SMILES	OCC1=CC2=C(C(O)=C1O)C(=O)C1=C(C(O)=C(O)C=C1O)C2=O
Stereochemistry	fully defined
Cross-references	npatlas:NPA05176

Filing pathway

Polyketides — computed by NPClassifier npclassifier.gnps2.org@2026-09-07, which is why it is pinned rather than recomputed. The gene cluster's asserted class is pks, from MIBiG.

Producer organisms 1

A claim that this taxon makes the compound. The basis says what the evidence addressed — a knockout and a database assertion are not the same claim.

Taxon	Basis	Cluster	Evidence
Aspergillus nidulans FGSC A4 NCBITaxon:227321	SOURCE_ASSERTION	mibig:BGC0000684	PMID:18978088

Occurrences 2

Somebody found the compound in this organism and cited it. That is not a claim that the organism makes it, and nothing here promotes one to the other.

Taxon	Source	Reference
Aspergillus nidulans NCBITaxon:162425	LOTUS	DOI:10.1128/AEM.01743-08
Aspergillus quadrilineatus NCBITaxon:41735	LOTUS	DOI:10.1042/BJ0590475

Biosynthetic gene clusters 1

Accession	Host	Locus evidence	Genome
mibig:BGC0000684	Aspergillus nidulans FGSC A4 NCBITaxon:227321	CLUSTER_UNSTATED	genbank:BN001301.1

A locus claim and a taxon claim are different questions. Heterologous expression settles the first and leaves the second where the isolation report left it.

Mechanism & Biosynthesis Overview

Causal graph — biosynthesis 5 nodes, 4 edges

Genetics-supported asperthecin production with AptA/B/C FGSC A4 reference proteins. Gene deletions in the 2021 laboratory strains remove the assigned asperthecin signal during sexual development. The reference proteins are not asserted to be the exact experimental alleles. No complete pathway, reaction order, immediate intermediates, native chain-shortening enzyme, protein complex or direct UV-protection mechanism is assigned. The source-imported producer and BGC grades remain unchanged.

Subject	Predicate	Object	Notes	Evidence
AptA (FGSC A4 reference)	has_gene_deletion_support_for	Asperthecin production during sexual development	The node denotes the native FGSC A4 reference for the named gene. The evidence concerns a gene-deletion phenotype in laboratory backgrounds, not a purified-protein reaction or proof of identical alleles in every tested strain.	DOI:10.1093/jimb/kuab055 https://www.uniprot.org/uniprotkb/Q5B0D0/entry https://www.ncbi.nlm.nih.gov/protein/CBF70387.1 reference DOI:10.1093/jimb/kuab055 evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-11 notes Palmer et al. 2021, Table 1, Methods, pp5-7 and Fig.4: the aptA-deletion line RJMP240.8 is listed in a veA+ background. The authors crossed RDIT55.37 with the corresponding earlier deletion line to generate the study strains; they report loss of asperthecin in the aptA deletion samples. Cleistothecial extracts after sexual induction on Champe medium at 37 C in darkness were compared with an asperthecin standard by HPLC-DAD; the article also reports mass-spectrometric identification. This supports involvement in production under those conditions, not sufficiency of three purified enzymes or a specific substrate/product step. The FGSC A4 canonical accession is an explicitly selected reference, not the sequenced complete experimental allele. reference https://www.uniprot.org/uniprotkb/Q5B0D0/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Fails to produce asperthecin notes Reviewed UniProtKB entry version 129, sequence version 1, disruption-phenotype annotation; the function comment identifies aptA as part of the asperthecin cluster. The shared detailed pathway paragraph is not adopted as a complete native reaction sequence. AptB cross-pathway Claisen chemistry is not assigned as a native asperthecin-forming step. reference https://www.ncbi.nlm.nih.gov/protein/CBF70387.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes Native FGSC A4 (NCBITaxon:227321) reference ANIA_06000, aptA, 1792 aa; join(BN001301.1:1542669..1542985, BN001301.1:1543039..1544140,BN001301.1:1544202..1546844, BN001301.1:1546898..1548214). CDS-aware genomic translation with intron splicing, deposited translation, independent GenPept and reviewed UniProt sequences match exactly. The 2021 paper explicitly used the FGSC A4 reference genome for SNP analysis, but its deletion lines and historical mutant alleles are not equated with the canonical sequence. No engineered tag, purified assay construct, precursor processing or physical protein complex is inferred.
AptB (FGSC A4 reference)	has_gene_deletion_support_for	Asperthecin production during sexual development	The node denotes the native FGSC A4 reference for the named gene. The evidence concerns a gene-deletion phenotype in laboratory backgrounds, not a purified-protein reaction or proof of identical alleles in every tested strain.	DOI:10.1093/jimb/kuab055 https://www.uniprot.org/uniprotkb/Q5B0C9/entry https://www.ncbi.nlm.nih.gov/protein/CBF70385.1 reference DOI:10.1093/jimb/kuab055 evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-11 notes Palmer et al. 2021, Table 1, Methods, pp5-7 and Fig.4: the aptB-deletion line RJMP238.5 is listed in a veA+ background. The authors crossed RDIT55.37 with the corresponding earlier deletion line to generate the study strains; they report loss of asperthecin in the aptB deletion samples. Cleistothecial extracts after sexual induction on Champe medium at 37 C in darkness were compared with an asperthecin standard by HPLC-DAD; the article also reports mass-spectrometric identification. This supports involvement in production under those conditions, not sufficiency of three purified enzymes or a specific substrate/product step. The FGSC A4 canonical accession is an explicitly selected reference, not the sequenced complete experimental allele. reference https://www.uniprot.org/uniprotkb/Q5B0C9/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Fails to produce asperthecin notes Reviewed UniProtKB entry version 108, sequence version 1, disruption-phenotype annotation; the function comment identifies aptB as part of the asperthecin cluster. The shared detailed pathway paragraph is not adopted as a complete native reaction sequence. AptB cross-pathway Claisen chemistry is not assigned as a native asperthecin-forming step. reference https://www.ncbi.nlm.nih.gov/protein/CBF70385.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes Native FGSC A4 (NCBITaxon:227321) reference ANIA_06001, aptB, 309 aa; complement(join(BN001301.1:1541240..1541959, BN001301.1:1542027..1542236)). CDS-aware genomic translation with intron splicing, deposited translation, independent GenPept and reviewed UniProt sequences match exactly. The 2021 paper explicitly used the FGSC A4 reference genome for SNP analysis, but its deletion lines and historical mutant alleles are not equated with the canonical sequence. No engineered tag, purified assay construct, precursor processing or physical protein complex is inferred.
AptC (FGSC A4 reference)	has_gene_deletion_support_for	Asperthecin production during sexual development	The node denotes the native FGSC A4 reference for the named gene. The evidence concerns a gene-deletion phenotype in laboratory backgrounds, not a purified-protein reaction or proof of identical alleles in every tested strain.	DOI:10.1093/jimb/kuab055 https://www.uniprot.org/uniprotkb/Q5B0C8/entry https://www.ncbi.nlm.nih.gov/protein/CBF70383.1 reference DOI:10.1093/jimb/kuab055 evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-11 notes Palmer et al. 2021, Table 1, Methods, pp5-7 and Fig.4: the aptC-deletion line RJMP239.7 is listed in a veA+ background. The authors crossed RDIT55.37 with the corresponding earlier deletion line to generate the study strains; they report loss of asperthecin in the aptC deletion samples. Cleistothecial extracts after sexual induction on Champe medium at 37 C in darkness were compared with an asperthecin standard by HPLC-DAD; the article also reports mass-spectrometric identification. This supports involvement in production under those conditions, not sufficiency of three purified enzymes or a specific substrate/product step. The FGSC A4 canonical accession is an explicitly selected reference, not the sequenced complete experimental allele. reference https://www.uniprot.org/uniprotkb/Q5B0C8/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 snippet Fails to produce asperthecin notes Reviewed UniProtKB entry version 112, sequence version 1, disruption-phenotype annotation; the function comment identifies aptC as part of the asperthecin cluster. The shared detailed pathway paragraph is not adopted as a complete native reaction sequence. AptB cross-pathway Claisen chemistry is not assigned as a native asperthecin-forming step. reference https://www.ncbi.nlm.nih.gov/protein/CBF70383.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes Native FGSC A4 (NCBITaxon:227321) reference ANIA_06002, aptC, 417 aa; BN001301.1:1539841..1541094. CDS-aware genomic translation with intron splicing, deposited translation, independent GenPept and reviewed UniProt sequences match exactly. The 2021 paper explicitly used the FGSC A4 reference genome for SNP analysis, but its deletion lines and historical mutant alleles are not equated with the canonical sequence. No engineered tag, purified assay construct, precursor processing or physical protein complex is inferred.
Asperthecin production during sexual development	produces	Asperthecin		DOI:10.1093/jimb/kuab055 reference DOI:10.1093/jimb/kuab055 evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-11 snippet we were able to consistently identify asperthecin notes Palmer et al. 2021 pp5-7 and Fig.4: the wild-type extract contains the peak assigned by comparison with an asperthecin standard; the discussion reports HPLC and mass-spectrometric identification. This is the compound-level output of the observed process, not an additional AptC-catalyzed final reaction. Unidentified pigments, hypothetical polymers and ascoquinone A are not equated with the exact owner.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 4 nodes, 3 edges

Developmental-gene perturbations associated with asperthecin production. NsdD deletion lacks detectable asperthecin and cleistothecia; MAT1/MatB deletion increases asperthecin despite absent ascospores. The nodes are sequence-verified FGSC A4 references, not complete experimental alleles. Genetic effects are not equated with direct apt-promoter regulation, a catalytic reaction, a regulatory protein complex or universal production requirements. The source-imported producer and BGC grades are unchanged.

Subject	Predicate	Object	Notes	Evidence
NsdD (FGSC A4 reference)	has_deletion_associated_loss_of	Asperthecin production under sexual-induction conditions	The protein node is an explicitly selected native reference for the named developmental gene. The edge concerns a laboratory gene-deletion phenotype, not the direct molecular action of the canonical protein.	DOI:10.1093/jimb/kuab055 https://www.uniprot.org/uniprotkb/G5EB07/entry https://www.ncbi.nlm.nih.gov/protein/CBF83303.1 reference DOI:10.1093/jimb/kuab055 evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-11 snippet knockout strains of two genes involved in sexual development notes Palmer et al. 2021, Table 1, Methods and Fig.4/p6: Table 1 lists the nsdD-deletion strain RDIT88.13 in a veA+ background. The nsdD-deletion profile lacks the assigned asperthecin peak, and Fig.4 also labels this genotype as lacking cleistothecia and ascospores. This is a developmental-context-associated loss of detectable metabolite, not proof of a direct effect on an apt promoter or of a missing catalytic step. The extraction/analytical workflow uses sexual-induction conditions, Champe medium at 37 C in darkness for 18 days and HPLC-DAD comparisons to an asperthecin standard; the article also reports mass-spectrometric identification. No fold change, detection limit or universal biosynthetic essentiality is inferred. reference https://www.uniprot.org/uniprotkb/G5EB07/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Unreviewed UniProtKB/TrEMBL native reference, entry version 77, sequence version 1, gene nsdD, ANIA_03152, 461 aa, NCBITaxon:227321. Protein-existence annotation remains 4: Predicted. This entry supplies reference identity, not an independently curated asperthecin mechanism or proof of the complete laboratory allele. reference https://www.ncbi.nlm.nih.gov/protein/CBF83303.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes Native FGSC A4 reference ANIA_03152, nsdD, 461 aa, chromosome VI: complement(join(BN001306.1:1594943..1595152, BN001306.1:1595209..1595538,BN001306.1:1595588..1596401, BN001306.1:1596476..1596507)). Reverse-strand CDS translation with 4 spliced exons exactly matches the deposited translation, independently retrieved GenPept and UniProt sequences. This is reference-sequence consistency, not independent sequencing of the experimental mutant or its parent. Both references lie outside the imported apt-cluster interval on BN001301.1. No engineered construct, direct DNA-binding target or protein complex is inferred.
MAT1/MatB (FGSC A4 reference)	has_deletion_associated_increase_in	Asperthecin production under sexual-induction conditions	The protein node is an explicitly selected native reference for the named developmental gene. The edge concerns a laboratory gene-deletion phenotype, not the direct molecular action of the canonical protein.	DOI:10.1093/jimb/kuab055 https://www.uniprot.org/uniprotkb/G5EAT5/entry https://www.ncbi.nlm.nih.gov/protein/CBF84081.1 https://www.ncbi.nlm.nih.gov/gene/2874476 reference DOI:10.1093/jimb/kuab055 evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-11 snippet an overproduction of asperthecin notes Palmer et al. 2021, Table 1 and Fig.4/p6: the article calls the alpha-box gene MAT-1 (matB), reports increased asperthecin in the matB-deletion profile, and labels that genotype as having cleistothecia but no ascospores. Table 1 lists two single matB-deletion backgrounds, TJMP190.4 and RJMP290.2, plus the matB/aptC double deletion RJMP291.12. Fig.4 labels genotypes rather than an unambiguous single-strain identifier, so the plotted single deletion is not assigned uniquely to either Table 1 line here. The double-deletion profile resembles aptC deletion, not restoration of final asperthecin. HPLC-DAD comparisons use an asperthecin standard under the stated sexual-induction conditions. This is not a quantitative fold-change claim or evidence that MAT1 directly represses apt transcription. reference https://www.uniprot.org/uniprotkb/G5EAT5/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Unreviewed UniProtKB/TrEMBL native reference, entry version 61, sequence version 1, gene MAT1, ANIA_02755, 361 aa, NCBITaxon:227321. Protein-existence annotation remains 3: Inferred from homology. This entry supplies reference identity, not an independently curated asperthecin mechanism or proof of the complete laboratory allele. reference https://www.ncbi.nlm.nih.gov/protein/CBF84081.1 evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes Native FGSC A4 reference ANIA_02755, MAT1, 361 aa, chromosome VI: complement(join(BN001306.1:2857276..2858072, BN001306.1:2858129..2858417)). Reverse-strand CDS translation with 2 spliced exons exactly matches the deposited translation, independently retrieved GenPept and UniProt sequences. This is reference-sequence consistency, not independent sequencing of the experimental mutant or its parent. Both references lie outside the imported apt-cluster interval on BN001301.1. No engineered construct, direct DNA-binding target or protein complex is inferred. reference https://www.ncbi.nlm.nih.gov/gene/2874476 evidence type CURATOR_INFERENCE retrieved on 2026-10-11 notes NCBI Gene identifies MAT1/ANIA_02755. The independently retrieved native GenPept product is named MAT-1 and an alpha-box protein, matching the paper's explicit MAT-1 (matB) terminology. The Gene record itself provides no matB synonym. This supports the selected native reference by gene name and protein class, not a sequenced mapping of the plotted deletion allele or its construction primers.
Asperthecin production under sexual-induction conditions	produces	Asperthecin		DOI:10.1093/jimb/kuab055 reference DOI:10.1093/jimb/kuab055 evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-11 notes Fig.4 and the discussion identify the asperthecin signal in positive extracts by comparison with the standard and report HPLC/mass-spectrometric identification. This identifies the measured compound in the production process; it does not say every mutant produces it or that either regulator catalyzes its formation. Unknown pigments and hypothetical polymers are not equated with CHEBI:64161.

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Causal graph — biosynthesis 12 nodes, 8 edges

Partial view of four distinct regulatory perturbations affecting asperthecin production. Mediation links among these experiments and the AptABC biosynthetic machinery are intentionally omitted because they are not established here. FGSC A4 accessions identify native reference proteins, not verified experimental alleles. AflR deletion is in an OE:rsmA background; NapA is overexpressed, not deleted; AtnN deletion is tested in Czapek-Dox broth. Those three outcomes are bounded to inspected primary abstracts. LaeB disruption is an exact-compound UniProt annotation only. No direct apt-promoter binding, physical complex, reaction step, quantitative titer or universal effect is asserted.

Subject	Predicate	Object	Notes	Evidence
AflR (FGSC A4 native reference)	is_native_reference_for_targeted_gene_in	aflR deletion in an OE:rsmA background	The accession identifies a native FGSC A4 reference for the named gene, not the disrupted or overexpressed experimental allele. Identity mapping is not a claim that native protein abundance or direct activity was measured.	https://www.uniprot.org/uniprotkb/P52957/entry https://www.ncbi.nlm.nih.gov/protein/CBF80174.1 reference https://www.uniprot.org/uniprotkb/P52957/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed reference ANIA_07820, taxon227321; entry 136, sequence 2, annotation 2026-09-02. Gene-to-claim cross-reference PMID22283524; database citation does not by itself constitute primary evidence. reference https://www.ncbi.nlm.nih.gov/protein/CBF80174.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes Identity only: CBF80174.1, ANIA_07820, 433 aa matches reviewed UniProt and complete spliced CDS on BN001304.1:2718656..2719957 (1 exon(s), strand 1), including start/stop. Native FGSC A4 taxon227321; exact experimental allele and strain equivalence remain unverified.
aflR deletion in an OE:rsmA background	results_in	Reduced asperthecin synthesis in the tested OE:rsmA background		DOI:10.1111/j.1365-2958.2012.07986.x reference DOI:10.1111/j.1365-2958.2012.07986.x evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-12 notes Only the author abstract indexed at https://europepmc.org/article/MED/22283524 was inspected. Full Methods, figures, experimental strain and allele sequence remain unverified. No quantitative titer or universal phenotype is asserted. The abstract specifically reports reduced asperthecin after aflR deletion in an overexpression rsmA (OE:rsmA) background. Not a basal wild-type deletion result. AflR promoter binding and sterigmatocystin regulation do not establish direct binding to asperthecin-cluster promoters. snippet significantly reduces asperthecin synthesis
NapA (FGSC A4 native reference)	is_native_reference_for_targeted_gene_in	napA overexpression	The accession identifies a native FGSC A4 reference for the named gene, not the disrupted or overexpressed experimental allele. Identity mapping is not a claim that native protein abundance or direct activity was measured.	https://www.uniprot.org/uniprotkb/Q5AW17/entry https://www.ncbi.nlm.nih.gov/protein/CBF79538.1 reference https://www.uniprot.org/uniprotkb/Q5AW17/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed reference ANIA_07513, taxon227321; entry 125, sequence 1, annotation 2026-09-02. Gene-to-claim cross-reference PMID23154967; database citation does not by itself constitute primary evidence. reference https://www.ncbi.nlm.nih.gov/protein/CBF79538.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes Identity only: CBF79538.1, ANIA_07513, 577 aa matches reviewed UniProt and complete spliced CDS on BN001304.1:1734467..1736306 (3 exon(s), strand 1), including start/stop. Native FGSC A4 taxon227321; exact experimental allele and strain equivalence remain unverified.
napA overexpression	results_in	Decreased asperthecin production in the tested napA-overexpression culture		DOI:10.1099/mic.0.063370-0 reference DOI:10.1099/mic.0.063370-0 evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-12 notes Only the author abstract indexed at https://europepmc.org/article/MED/23154967 was inspected. Full Methods, figures, experimental strain and allele sequence remain unverified. No quantitative titer or universal phenotype is asserted. The abstract reports decreased asperthecin in OE::napA. This is overexpression, not deletion; no knockout-increase claim is inferred. The 100-fold change reported for OE::rsmA sterigmatocystin is not transferred to NapA or asperthecin. FRET self-association does not establish an asperthecin-regulatory complex. snippet OE : : napA displayed decreased production of sterigmatocystin, emericellin, asperthecin
AtnN (FGSC A4 native reference)	is_native_reference_for_targeted_gene_in	atnN deletion in Czapek-Dox broth	The accession identifies a native FGSC A4 reference for the named gene, not the disrupted or overexpressed experimental allele. Identity mapping is not a claim that native protein abundance or direct activity was measured.	https://www.uniprot.org/uniprotkb/C8V3N0/entry https://www.ncbi.nlm.nih.gov/protein/CBF73428.1 reference https://www.uniprot.org/uniprotkb/C8V3N0/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed reference ANIA_07872, taxon227321; entry 69, sequence 1, annotation 2026-09-02. Gene-to-claim cross-reference PMID39643398; database citation does not by itself constitute primary evidence. Reject old EAA58917.1: UniProt warns of an erroneous gene model. reference https://www.ncbi.nlm.nih.gov/protein/CBF73428.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes Identity only: CBF73428.1, ANIA_07872, 610 aa matches reviewed UniProt and complete spliced CDS on BN001302.1:74783..76663 (2 exon(s), strand -1), including start/stop. Native FGSC A4 taxon227321; exact experimental allele and strain equivalence remain unverified.
atnN deletion in Czapek-Dox broth	results_in	Increased asperthecin production in Czapek-Dox broth		DOI:10.1016/j.funbio.2024.11.006 reference DOI:10.1016/j.funbio.2024.11.006 evidence type PRIMARY_EXPERIMENT retrieved on 2026-10-12 notes Only the author abstract indexed at https://europepmc.org/article/MED/39643398 was inspected. Full Methods, figures, experimental strain and allele sequence remain unverified. No quantitative titer or universal phenotype is asserted. The abstract reports increased asperthecin in the atnN deletion mutant in Czapek-Dox Broth. Do not transfer the complex-medium atnN-overexpression siderophore results or minimal-medium sterigmatocystin results to this asperthecin observation. snippet increased asperthecin production was observed
LaeB (FGSC A4 native reference)	is_native_reference_for_targeted_gene_in	laeB disruption (database-annotated experiment)	The accession identifies a native FGSC A4 reference for the named gene, not the disrupted or overexpressed experimental allele. Identity mapping is not a claim that native protein abundance or direct activity was measured.	https://www.uniprot.org/uniprotkb/Q5B431/entry https://www.ncbi.nlm.nih.gov/protein/CBF76971.1 reference https://www.uniprot.org/uniprotkb/Q5B431/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes Reviewed reference ANIA_04699, taxon227321; entry 74, sequence 1, annotation 2026-09-02. Gene-to-claim cross-reference PMID29947411; database citation does not by itself constitute primary evidence. reference https://www.ncbi.nlm.nih.gov/protein/CBF76971.1 evidence type DATABASE_ASSERTION retrieved on 2026-10-12 notes Identity only: CBF76971.1, ANIA_04699, 650 aa matches reviewed UniProt and complete spliced CDS on BN001303.1:1252763..1255155 (3 exon(s), strand -1), including start/stop. Native FGSC A4 taxon227321; exact experimental allele and strain equivalence remain unverified.
laeB disruption (database-annotated experiment)	annotated_to_result_in	Activation of asperthecin biosynthesis (database annotation)		https://www.uniprot.org/uniprotkb/Q5B431/entry reference https://www.uniprot.org/uniprotkb/Q5B431/entry evidence type DATABASE_ASSERTION retrieved on 2026-10-11 notes UniProt DISRUPTION PHENOTYPE annotates activation of asperthecin biosynthesis after laeB disruption, citing PMID29947411 / DOI10.1039/c8ob01326h. The inspected paper abstract does not name asperthecin; full text was not obtained. The exact-compound effect is DATABASE_ASSERTION, not independently inspected primary experimental evidence. No CsdA physical complex, splicing mechanism or direct apt-promoter contact is adopted. snippet leads to the activation of several secondary metabolism pathways

Every edge carries its own citation; an uncited edge is refused by the corpus tests.

Explore across X-Mech

AntibioticMech (asperthecin) ↗ MediaIngredientMech (CHEBI:64161) ↗ TaxonMech: Aspergillus nidulans FGSC A4 (NCBITaxon:227321) ↗ PathwayMech (mibig:BGC0000684) ↗

Open questions 3

curator-asperthecin-apt-reference-and-pathway-limits — Which native reactions and experimentally verified alleles connect AptA/B/C to the final asperthecin structure?

curator-asperthecin-developmental-regulator-limits — Which direct molecular connections, if any, link NsdD and MAT1/MatB to asperthecin production independently of developmental changes?

curator-asperthecin-regulator-perturbation-limits — Which experimental alleles, assay conditions and mediating steps establish the four regulator effects on asperthecin?

Provenance

Source concept	Source	Version
BGC0000684	MIBIG	4
CHEBI:64161	CHEBI	3-star

This page is generated from data/natural_products/polyketides/asperthecin.yaml, which is generated from the committed inventories. Neither is edited by hand.